Duck associated cyclovirus 1
Average proteome isoelectric point is 8.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0SVU5|A0A1Y0SVU5_9CIRC ATP-dependent helicase Rep OS=Duck associated cyclovirus 1 OX=2006585 PE=3 SV=1
MM1 pKa = 7.28 QRR3 pKa = 11.84 KK4 pKa = 9.22 CYY6 pKa = 10.43 SWTLNNYY13 pKa = 7.18 TSEE16 pKa = 4.63 DD17 pKa = 3.23 IEE19 pKa = 4.63 RR20 pKa = 11.84 IRR22 pKa = 11.84 QHH24 pKa = 5.54 EE25 pKa = 4.24 HH26 pKa = 6.15 LFDD29 pKa = 3.56 YY30 pKa = 10.48 AIWGRR35 pKa = 11.84 EE36 pKa = 3.71 IAPEE40 pKa = 3.97 TGTPHH45 pKa = 5.63 LQGYY49 pKa = 8.07 FKK51 pKa = 10.98 FKK53 pKa = 9.87 TKK55 pKa = 10.12 QRR57 pKa = 11.84 FNTVRR62 pKa = 11.84 AILGGRR68 pKa = 11.84 GHH70 pKa = 7.53 LEE72 pKa = 3.94 GSKK75 pKa = 10.81 GSPKK79 pKa = 10.08 QNHH82 pKa = 7.04 DD83 pKa = 3.66 YY84 pKa = 10.37 CAKK87 pKa = 10.09 SGDD90 pKa = 3.92 YY91 pKa = 8.45 EE92 pKa = 4.57 TVGDD96 pKa = 4.74 LVIASQSSLEE106 pKa = 4.6 LVCQMLKK113 pKa = 10.9 DD114 pKa = 4.14 GVRR117 pKa = 11.84 LAQVAADD124 pKa = 3.77 YY125 pKa = 10.21 PSIFVRR131 pKa = 11.84 HH132 pKa = 5.38 HH133 pKa = 6.08 RR134 pKa = 11.84 GLRR137 pKa = 11.84 EE138 pKa = 3.65 LSLILGVVKK147 pKa = 10.38 PRR149 pKa = 11.84 DD150 pKa = 3.57 FKK152 pKa = 9.43 TGKK155 pKa = 8.02 WAAARR160 pKa = 11.84 SIPEE164 pKa = 3.72 SLSRR168 pKa = 11.84 FRR170 pKa = 11.84 ANLLLTVRR178 pKa = 11.84 WMILSLLVLVSEE190 pKa = 4.4 VLVFYY195 pKa = 10.33 GASGTGKK202 pKa = 9.79 SRR204 pKa = 11.84 RR205 pKa = 11.84 AAEE208 pKa = 4.12 LCGSEE213 pKa = 3.79 STYY216 pKa = 10.88 YY217 pKa = 10.48 KK218 pKa = 10.5 PRR220 pKa = 11.84 GKK222 pKa = 9.0 WWDD225 pKa = 3.58 GYY227 pKa = 11.49 ANQDD231 pKa = 3.1 NVIIDD236 pKa = 4.57 DD237 pKa = 4.55 FYY239 pKa = 11.37 GWLSFDD245 pKa = 3.4 EE246 pKa = 4.63 FLRR249 pKa = 11.84 IADD252 pKa = 4.55 RR253 pKa = 11.84 YY254 pKa = 7.36 PCRR257 pKa = 11.84 VEE259 pKa = 4.22 VKK261 pKa = 10.58 GGFAEE266 pKa = 4.7 FTSKK270 pKa = 10.8 RR271 pKa = 11.84 IVITSNQAPSQWWRR285 pKa = 11.84 GEE287 pKa = 4.01 WFKK290 pKa = 11.79 GEE292 pKa = 3.85 QLKK295 pKa = 11.05 ALTRR299 pKa = 11.84 RR300 pKa = 11.84 LDD302 pKa = 3.54 VVCFYY307 pKa = 10.56 SAHH310 pKa = 6.41 SGLPHH315 pKa = 7.56 EE316 pKa = 5.5 INCQGDD322 pKa = 3.83 CDD324 pKa = 4.29 KK325 pKa = 11.23 EE326 pKa = 4.08
Molecular weight: 37.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.109
IPC2_protein 8.185
IPC_protein 8.068
Toseland 8.068
ProMoST 8.565
Dawson 8.814
Bjellqvist 9.004
Wikipedia 8.829
Rodwell 8.873
Grimsley 8.009
Solomon 8.931
Lehninger 8.916
Nozaki 9.136
DTASelect 8.785
Thurlkill 8.843
EMBOSS 8.99
Sillero 9.121
Patrickios 4.431
IPC_peptide 8.916
IPC2_peptide 7.907
IPC2.peptide.svr19 7.929
Protein with the highest isoelectric point:
>tr|A0A1Y0SVU5|A0A1Y0SVU5_9CIRC ATP-dependent helicase Rep OS=Duck associated cyclovirus 1 OX=2006585 PE=3 SV=1
MM1 pKa = 7.59 ANYY4 pKa = 9.47 RR5 pKa = 11.84 RR6 pKa = 11.84 KK7 pKa = 9.8 KK8 pKa = 10.13 KK9 pKa = 9.61 YY10 pKa = 8.71 GRR12 pKa = 11.84 RR13 pKa = 11.84 YY14 pKa = 8.67 RR15 pKa = 11.84 PRR17 pKa = 11.84 PLRR20 pKa = 11.84 VRR22 pKa = 11.84 LYY24 pKa = 8.91 NPKK27 pKa = 10.03 RR28 pKa = 11.84 NRR30 pKa = 11.84 LRR32 pKa = 11.84 RR33 pKa = 11.84 TQRR36 pKa = 11.84 RR37 pKa = 11.84 LKK39 pKa = 9.22 NDD41 pKa = 2.74 KK42 pKa = 10.26 YY43 pKa = 9.93 FTRR46 pKa = 11.84 FMSWTSVDD54 pKa = 3.39 VDD56 pKa = 3.73 PKK58 pKa = 10.62 IGQGWYY64 pKa = 9.78 LAPNYY69 pKa = 9.84 KK70 pKa = 10.41 SNPGFLQLAEE80 pKa = 4.24 QYY82 pKa = 11.09 SEE84 pKa = 4.22 FKK86 pKa = 9.2 IHH88 pKa = 6.58 KK89 pKa = 7.81 VAARR93 pKa = 11.84 ITPGLNEE100 pKa = 4.33 TYY102 pKa = 10.43 LAEE105 pKa = 4.05 QGLRR109 pKa = 11.84 YY110 pKa = 9.65 AYY112 pKa = 10.33 CPWQQDD118 pKa = 3.44 NGGSPSTNDD127 pKa = 2.71 IVSNGTDD134 pKa = 3.28 LTYY137 pKa = 11.02 GKK139 pKa = 10.62 LMNVPWAKK147 pKa = 10.12 SKK149 pKa = 10.61 RR150 pKa = 11.84 LHH152 pKa = 5.58 QSATIVYY159 pKa = 9.61 KK160 pKa = 9.69 PKK162 pKa = 10.36 CNQAIAFAPRR172 pKa = 11.84 TSSGANGNQRR182 pKa = 11.84 VISFPWVTTQAMSMGALPGMSGIMLAFDD210 pKa = 3.5 QQDD213 pKa = 3.73 PLSVIGKK220 pKa = 6.49 PLRR223 pKa = 11.84 VQVEE227 pKa = 4.05 FFLYY231 pKa = 8.66 VTFRR235 pKa = 11.84 KK236 pKa = 9.59 RR237 pKa = 11.84 RR238 pKa = 11.84 NPMLQTPTPFVMDD251 pKa = 3.16 QQ252 pKa = 2.98
Molecular weight: 29.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 10.014
IPC_protein 10.701
Toseland 10.628
ProMoST 10.335
Dawson 10.774
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 11.082
Grimsley 10.847
Solomon 10.847
Lehninger 10.804
Nozaki 10.599
DTASelect 10.482
Thurlkill 10.643
EMBOSS 11.023
Sillero 10.687
Patrickios 10.745
IPC_peptide 10.847
IPC2_peptide 9.385
IPC2.peptide.svr19 8.306
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
578
252
326
289.0
33.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.401 ± 0.033
1.73 ± 0.584
4.498 ± 0.578
4.325 ± 1.46
4.498 ± 0.083
7.439 ± 0.68
1.903 ± 0.692
4.325 ± 0.47
6.228 ± 0.323
8.478 ± 0.585
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.076 ± 0.932
4.152 ± 1.122
5.017 ± 1.573
5.017 ± 0.83
8.651 ± 0.545
6.92 ± 0.604
5.017 ± 0.583
5.882 ± 0.204
2.595 ± 0.381
4.844 ± 0.443
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here