Gordonia phage Obliviate

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142KAK8|A0A142KAK8_9CAUD Uncharacterized protein OS=Gordonia phage Obliviate OX=1821559 GN=62 PE=4 SV=1
MM1 pKa = 7.91TDD3 pKa = 2.89TTDD6 pKa = 4.91PIVPTCSLCQRR17 pKa = 11.84EE18 pKa = 4.3RR19 pKa = 11.84TLADD23 pKa = 3.64AYY25 pKa = 10.67DD26 pKa = 4.01YY27 pKa = 11.75NPLQVITGQPVGWYY41 pKa = 9.96SGDD44 pKa = 3.69DD45 pKa = 3.86GEE47 pKa = 5.54VCPQCMAKK55 pKa = 8.99TLNGQVV61 pKa = 3.1

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142KAI3|A0A142KAI3_9CAUD Uncharacterized protein OS=Gordonia phage Obliviate OX=1821559 GN=37 PE=4 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84FGQRR6 pKa = 11.84ALLDD10 pKa = 3.55RR11 pKa = 11.84YY12 pKa = 8.59VTYY15 pKa = 9.67EE16 pKa = 3.88AKK18 pKa = 10.24VVPVANDD25 pKa = 2.91IEE27 pKa = 4.63MTSHH31 pKa = 7.45VIEE34 pKa = 4.69DD35 pKa = 5.01WITHH39 pKa = 6.57LYY41 pKa = 10.98AEE43 pKa = 4.96GKK45 pKa = 9.64SNRR48 pKa = 11.84TVKK51 pKa = 10.42DD52 pKa = 3.07RR53 pKa = 11.84RR54 pKa = 11.84IVLRR58 pKa = 11.84RR59 pKa = 11.84FEE61 pKa = 4.68RR62 pKa = 11.84DD63 pKa = 2.6IEE65 pKa = 4.42TPILAATTGQIAAWLGRR82 pKa = 11.84DD83 pKa = 3.57DD84 pKa = 4.62LATVTRR90 pKa = 11.84SVYY93 pKa = 10.58HH94 pKa = 6.5SILTAFYY101 pKa = 9.37KK102 pKa = 9.9WAIAVKK108 pKa = 10.37LRR110 pKa = 11.84DD111 pKa = 4.56DD112 pKa = 4.22NPVAPIKK119 pKa = 10.04AAKK122 pKa = 9.6RR123 pKa = 11.84PRR125 pKa = 11.84RR126 pKa = 11.84QPRR129 pKa = 11.84PVTPEE134 pKa = 3.31QYY136 pKa = 10.32RR137 pKa = 11.84RR138 pKa = 11.84LLEE141 pKa = 4.26GAADD145 pKa = 4.09DD146 pKa = 4.23PVITAMLLLAGLQGLRR162 pKa = 11.84VHH164 pKa = 6.51EE165 pKa = 4.3VARR168 pKa = 11.84FHH170 pKa = 7.17SRR172 pKa = 11.84HH173 pKa = 6.09LDD175 pKa = 3.17AEE177 pKa = 4.11ARR179 pKa = 11.84TIEE182 pKa = 4.11VTGKK186 pKa = 10.41GGSSYY191 pKa = 11.01VLPAAQPVIDD201 pKa = 4.09HH202 pKa = 6.87ARR204 pKa = 11.84RR205 pKa = 11.84MPQTGFWFPSQRR217 pKa = 11.84AKK219 pKa = 10.86HH220 pKa = 5.51LGGRR224 pKa = 11.84TVSEE228 pKa = 4.67RR229 pKa = 11.84IHH231 pKa = 7.67LHH233 pKa = 5.49MIRR236 pKa = 11.84HH237 pKa = 5.78RR238 pKa = 11.84VPGTPHH244 pKa = 7.29CLRR247 pKa = 11.84HH248 pKa = 5.95HH249 pKa = 7.24FGTQLVSNGADD260 pKa = 2.95LRR262 pKa = 11.84VAQEE266 pKa = 4.07LLRR269 pKa = 11.84HH270 pKa = 5.97SSLQTTAIYY279 pKa = 10.64VATSDD284 pKa = 3.5DD285 pKa = 3.86RR286 pKa = 11.84KK287 pKa = 10.58RR288 pKa = 11.84EE289 pKa = 4.36AIDD292 pKa = 3.28RR293 pKa = 11.84LAAA296 pKa = 4.24

Molecular weight: 33.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

16063

52

1847

200.8

21.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.53 ± 0.519

0.89 ± 0.157

6.997 ± 0.344

5.559 ± 0.317

2.708 ± 0.132

8.653 ± 0.336

2.21 ± 0.19

4.445 ± 0.14

3.237 ± 0.246

7.863 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.166 ± 0.185

2.901 ± 0.167

5.889 ± 0.243

3.405 ± 0.154

7.433 ± 0.357

5.18 ± 0.205

6.96 ± 0.267

7.583 ± 0.211

2.11 ± 0.117

2.279 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski