Tortoise microvirus 111

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W848|A0A4P8W848_9VIRU Nonstructural protein OS=Tortoise microvirus 111 OX=2583112 PE=4 SV=1
MM1 pKa = 7.88KK2 pKa = 10.24YY3 pKa = 10.03RR4 pKa = 11.84RR5 pKa = 11.84PDD7 pKa = 3.41YY8 pKa = 10.9LEE10 pKa = 4.16KK11 pKa = 10.77QSFEE15 pKa = 3.91GTLYY19 pKa = 10.8SPYY22 pKa = 10.27EE23 pKa = 3.81PEE25 pKa = 3.9YY26 pKa = 10.87VLNEE30 pKa = 3.9EE31 pKa = 4.35TEE33 pKa = 4.07EE34 pKa = 4.29LEE36 pKa = 4.28IVGQINVQEE45 pKa = 4.51QIQSHH50 pKa = 6.58AGCALDD56 pKa = 5.22KK57 pKa = 10.81IFEE60 pKa = 4.44RR61 pKa = 11.84FLGDD65 pKa = 3.15IPQNNPVSTPVDD77 pKa = 3.34GVYY80 pKa = 9.38DD81 pKa = 3.69TTQRR85 pKa = 11.84ITDD88 pKa = 3.9LAEE91 pKa = 3.98LGKK94 pKa = 10.25ICDD97 pKa = 3.1IAEE100 pKa = 4.66GYY102 pKa = 9.61RR103 pKa = 11.84RR104 pKa = 11.84NNNLSAEE111 pKa = 4.14LSISQIFDD119 pKa = 4.47LISRR123 pKa = 11.84QEE125 pKa = 3.9KK126 pKa = 10.59SIAAYY131 pKa = 9.55IAAQTKK137 pKa = 10.46KK138 pKa = 10.79GDD140 pKa = 3.78NNNEE144 pKa = 3.97NEE146 pKa = 4.36KK147 pKa = 9.47KK148 pKa = 8.81TVAEE152 pKa = 4.87GEE154 pKa = 4.34QEE156 pKa = 4.3TIPTDD161 pKa = 3.77GEE163 pKa = 4.14

Molecular weight: 18.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W790|A0A4P8W790_9VIRU Major capsid protein OS=Tortoise microvirus 111 OX=2583112 PE=3 SV=1
MM1 pKa = 6.85VVCTVVDD8 pKa = 3.7KK9 pKa = 10.57LTGHH13 pKa = 6.19IQRR16 pKa = 11.84HH17 pKa = 4.46TVFNSPMADD26 pKa = 3.53SYY28 pKa = 12.05DD29 pKa = 3.62EE30 pKa = 4.6TPTKK34 pKa = 10.78VYY36 pKa = 10.19TRR38 pKa = 11.84VNTVVACGQCLEE50 pKa = 4.61CLQQRR55 pKa = 11.84SIEE58 pKa = 3.98WSHH61 pKa = 8.0RR62 pKa = 11.84IMDD65 pKa = 3.98EE66 pKa = 4.04VSLHH70 pKa = 5.87NSCCFLTLTYY80 pKa = 10.58KK81 pKa = 10.81DD82 pKa = 3.64NPVNLVKK89 pKa = 10.25RR90 pKa = 11.84DD91 pKa = 3.34LQLFVKK97 pKa = 10.32RR98 pKa = 11.84LRR100 pKa = 11.84KK101 pKa = 9.66ALEE104 pKa = 3.96PQKK107 pKa = 10.49IRR109 pKa = 11.84YY110 pKa = 7.01FACGEE115 pKa = 4.12YY116 pKa = 10.01GAKK119 pKa = 9.7RR120 pKa = 11.84ARR122 pKa = 11.84PHH124 pKa = 4.51YY125 pKa = 10.69HH126 pKa = 5.96MIIFGWRR133 pKa = 11.84PDD135 pKa = 3.39DD136 pKa = 4.04LEE138 pKa = 4.58YY139 pKa = 11.04FFTDD143 pKa = 2.64AHH145 pKa = 6.18GFAVYY150 pKa = 9.6QSPFVAEE157 pKa = 4.26RR158 pKa = 11.84WSLGYY163 pKa = 8.8VTVGDD168 pKa = 4.02VTLEE172 pKa = 4.01TAKK175 pKa = 10.95YY176 pKa = 7.95CAKK179 pKa = 10.31YY180 pKa = 9.81LQKK183 pKa = 10.99LQDD186 pKa = 3.69TDD188 pKa = 3.71GLEE191 pKa = 4.25PPFLLMSTRR200 pKa = 11.84PGIGYY205 pKa = 9.51GVIDD209 pKa = 4.31EE210 pKa = 4.8SMLVDD215 pKa = 3.76DD216 pKa = 6.23KK217 pKa = 11.24IYY219 pKa = 11.1HH220 pKa = 5.9EE221 pKa = 4.72GNYY224 pKa = 9.65IKK226 pKa = 10.74LPRR229 pKa = 11.84YY230 pKa = 7.22YY231 pKa = 10.5CKK233 pKa = 10.58VLASRR238 pKa = 11.84CPDD241 pKa = 2.96KK242 pKa = 11.25LAVLKK247 pKa = 8.46EE248 pKa = 3.79QRR250 pKa = 11.84VRR252 pKa = 11.84KK253 pKa = 9.69AKK255 pKa = 10.4LLEE258 pKa = 4.26RR259 pKa = 11.84THH261 pKa = 6.75TEE263 pKa = 3.42LQARR267 pKa = 11.84RR268 pKa = 11.84EE269 pKa = 4.01EE270 pKa = 4.17LQARR274 pKa = 11.84LGAIRR279 pKa = 11.84CPSRR283 pKa = 11.84RR284 pKa = 11.84KK285 pKa = 9.08KK286 pKa = 11.1SKK288 pKa = 9.33MKK290 pKa = 10.43KK291 pKa = 9.52

Molecular weight: 33.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1240

75

557

248.0

27.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.226 ± 0.927

1.452 ± 0.747

5.726 ± 0.08

5.161 ± 1.717

3.548 ± 0.505

6.855 ± 0.745

1.694 ± 0.381

4.516 ± 0.785

3.871 ± 1.191

8.065 ± 0.621

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.935 ± 0.257

4.435 ± 0.779

4.839 ± 0.573

4.839 ± 0.393

6.29 ± 0.773

8.145 ± 1.551

6.048 ± 0.09

7.419 ± 0.858

1.129 ± 0.287

5.806 ± 0.517
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski