Podoviridae sp. cty5g4
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2WB09|A0A5Q2WB09_9CAUD Uncharacterized protein OS=Podoviridae sp. cty5g4 OX=2656717 PE=4 SV=1
MM1 pKa = 7.58 KK2 pKa = 10.34 YY3 pKa = 10.01 IYY5 pKa = 10.14 TMPDD9 pKa = 3.23 CPRR12 pKa = 11.84 CDD14 pKa = 3.65 VLKK17 pKa = 10.66 KK18 pKa = 10.49 RR19 pKa = 11.84 YY20 pKa = 9.43 IEE22 pKa = 4.35 DD23 pKa = 4.17 GIMFTEE29 pKa = 4.61 RR30 pKa = 11.84 NADD33 pKa = 3.54 RR34 pKa = 11.84 MKK36 pKa = 10.96 SPEE39 pKa = 3.97 DD40 pKa = 3.84 TIDD43 pKa = 3.45 TEE45 pKa = 4.56 ALIQASMQNMEE56 pKa = 4.85 LPVEE60 pKa = 4.13 IEE62 pKa = 4.12 VEE64 pKa = 4.16 KK65 pKa = 10.65 TEE67 pKa = 4.62 SDD69 pKa = 3.37 FDD71 pKa = 3.53 ICQSCEE77 pKa = 3.94 YY78 pKa = 9.3 YY79 pKa = 10.34 PCDD82 pKa = 3.47 SAPNADD88 pKa = 3.64 YY89 pKa = 11.24 CSVRR93 pKa = 11.84 EE94 pKa = 4.11
Molecular weight: 10.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.916
IPC2_protein 4.202
IPC_protein 4.113
Toseland 3.935
ProMoST 4.202
Dawson 4.075
Bjellqvist 4.279
Wikipedia 3.973
Rodwell 3.948
Grimsley 3.846
Solomon 4.062
Lehninger 4.024
Nozaki 4.19
DTASelect 4.368
Thurlkill 3.961
EMBOSS 3.986
Sillero 4.228
Patrickios 1.036
IPC_peptide 4.075
IPC2_peptide 4.215
IPC2.peptide.svr19 4.144
Protein with the highest isoelectric point:
>tr|A0A5Q2W5K2|A0A5Q2W5K2_9CAUD Uncharacterized protein OS=Podoviridae sp. cty5g4 OX=2656717 PE=4 SV=1
MM1 pKa = 7.21 WRR3 pKa = 11.84 VVWAGILLPGSPAHH17 pKa = 5.86 TCNLAFRR24 pKa = 11.84 RR25 pKa = 11.84 HH26 pKa = 4.07 KK27 pKa = 9.6 TGWQISTTTALVFGQATGYY46 pKa = 10.41 LL47 pKa = 3.81
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.999
IPC_protein 11.038
Toseland 10.994
ProMoST 11.067
Dawson 11.067
Bjellqvist 10.921
Wikipedia 11.389
Rodwell 11.082
Grimsley 11.125
Solomon 11.345
Lehninger 11.286
Nozaki 10.994
DTASelect 10.906
Thurlkill 10.994
EMBOSS 11.433
Sillero 11.023
Patrickios 11.023
IPC_peptide 11.345
IPC2_peptide 10.321
IPC2.peptide.svr19 8.352
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
85
0
85
16379
29
1299
192.7
21.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.168 ± 0.327
1.343 ± 0.175
5.11 ± 0.304
6.661 ± 0.389
3.553 ± 0.153
7.247 ± 0.401
1.545 ± 0.133
7.821 ± 0.309
7.638 ± 0.512
7.693 ± 0.337
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.467 ± 0.171
5.312 ± 0.337
4.133 ± 0.307
3.413 ± 0.298
4.414 ± 0.233
6.514 ± 0.298
6.527 ± 0.413
5.995 ± 0.227
1.38 ± 0.167
4.066 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here