Mycobacterium haemophilum

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3743 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0I9TV89|A0A0I9TV89_9MYCO Esterase OS=Mycobacterium haemophilum OX=29311 GN=ABH38_02245 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.21YY4 pKa = 11.53KK5 pKa = 10.95LFRR8 pKa = 11.84CVQCGFEE15 pKa = 3.99YY16 pKa = 10.91DD17 pKa = 3.98EE18 pKa = 4.57AQGWPEE24 pKa = 4.38DD25 pKa = 4.2GIEE28 pKa = 4.69PGTRR32 pKa = 11.84WDD34 pKa = 5.75DD35 pKa = 3.57IPEE38 pKa = 4.28DD39 pKa = 4.18WSCPDD44 pKa = 3.79CGAAKK49 pKa = 10.43ADD51 pKa = 3.7FEE53 pKa = 4.55MVEE56 pKa = 4.25VARR59 pKa = 11.84PP60 pKa = 3.22

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0I9UPP1|A0A0I9UPP1_9MYCO UPF0678 fatty acid-binding protein-like protein ABH38_03365 OS=Mycobacterium haemophilum OX=29311 GN=ABH38_03365 PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84GFSQNTNRR10 pKa = 11.84PRR12 pKa = 11.84AIHH15 pKa = 6.53ARR17 pKa = 11.84TISWWVSSAVATTTASTSALFTQSRR42 pKa = 11.84GSWKK46 pKa = 8.39TAAVGTAAAARR57 pKa = 11.84SANGII62 pKa = 3.85

Molecular weight: 6.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3743

0

3743

1208662

29

4163

322.9

34.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.097 ± 0.053

0.873 ± 0.011

5.994 ± 0.036

4.9 ± 0.035

2.922 ± 0.023

8.744 ± 0.051

2.282 ± 0.016

4.405 ± 0.027

2.204 ± 0.029

9.989 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.064 ± 0.016

2.352 ± 0.021

5.73 ± 0.03

3.264 ± 0.022

7.244 ± 0.048

5.601 ± 0.026

6.011 ± 0.026

8.675 ± 0.035

1.495 ± 0.017

2.152 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski