Tenuibacillus multivorans
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3018 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H0BSN0|A0A1H0BSN0_9BACI Uncharacterized protein OS=Tenuibacillus multivorans OX=237069 GN=SAMN05216498_2373 PE=4 SV=1
MM1 pKa = 7.93 PEE3 pKa = 3.85 PVTNKK8 pKa = 9.81 NVSSTDD14 pKa = 3.08 INEE17 pKa = 4.03 EE18 pKa = 3.86 ATGGVQNKK26 pKa = 7.55 ITWTDD31 pKa = 3.58 SISADD36 pKa = 3.16 VKK38 pKa = 10.4 SYY40 pKa = 10.57 QIHH43 pKa = 7.22 RR44 pKa = 11.84 STTSNPLDD52 pKa = 3.81 GKK54 pKa = 10.64 QLDD57 pKa = 4.98 GYY59 pKa = 10.27 IEE61 pKa = 5.11 PNTEE65 pKa = 3.98 TYY67 pKa = 11.05 TDD69 pKa = 3.4 TTANAGTTYY78 pKa = 9.51 YY79 pKa = 10.19 YY80 pKa = 10.63 HH81 pKa = 7.23 VGASDD86 pKa = 3.49 GTNINLGTAIQVDD99 pKa = 4.0 TTKK102 pKa = 10.89 DD103 pKa = 2.87 IDD105 pKa = 3.94 SAPTTISGITANDD118 pKa = 3.75 ADD120 pKa = 4.07 TSGNGTFVNVNFTNSTEE137 pKa = 4.18 SDD139 pKa = 3.5 FASYY143 pKa = 10.06 EE144 pKa = 4.22 VYY146 pKa = 10.27 IVASTFTGTTYY157 pKa = 11.36 NEE159 pKa = 3.94 VTNAAVNSVTSTSIEE174 pKa = 4.29 DD175 pKa = 3.72 GTNGLQVDD183 pKa = 4.13 AGANTGEE190 pKa = 4.08 FKK192 pKa = 11.14 VYY194 pKa = 10.48 VFSKK198 pKa = 9.76 DD199 pKa = 2.87 TAGQVSGMPTAANVTMGQQ217 pKa = 3.2
Molecular weight: 22.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.643
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.681
Grimsley 3.554
Solomon 3.834
Lehninger 3.795
Nozaki 3.973
DTASelect 4.202
Thurlkill 3.706
EMBOSS 3.795
Sillero 3.986
Patrickios 1.036
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|A0A1G9WCI4|A0A1G9WCI4_9BACI Uncharacterized protein OS=Tenuibacillus multivorans OX=237069 GN=SAMN05216498_0692 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.19 RR10 pKa = 11.84 KK11 pKa = 9.74 RR12 pKa = 11.84 NKK14 pKa = 8.96 VHH16 pKa = 6.76 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 8.78 TKK25 pKa = 10.39 NGRR28 pKa = 11.84 KK29 pKa = 8.66 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.2 KK37 pKa = 10.05 GRR39 pKa = 11.84 KK40 pKa = 8.66 VLSAA44 pKa = 4.05
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.416
IPC2_protein 11.111
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.457
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.179
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.995
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3018
0
3018
845311
24
3029
280.1
31.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.26 ± 0.041
0.581 ± 0.012
5.93 ± 0.032
7.853 ± 0.053
4.498 ± 0.039
6.604 ± 0.046
2.262 ± 0.022
7.953 ± 0.05
6.285 ± 0.048
9.552 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.836 ± 0.022
4.684 ± 0.037
3.519 ± 0.026
4.319 ± 0.038
4.027 ± 0.032
5.86 ± 0.034
5.25 ± 0.03
7.03 ± 0.039
1.0 ± 0.015
3.695 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here