Fly associated circular virus 5
Average proteome isoelectric point is 8.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BP99|A0A346BP99_9VIRU Putative capsid protein OS=Fly associated circular virus 5 OX=2293285 PE=4 SV=1
MM1 pKa = 7.74 PKK3 pKa = 10.35 AKK5 pKa = 10.05 RR6 pKa = 11.84 EE7 pKa = 4.23 SPAKK11 pKa = 10.17 DD12 pKa = 3.24 YY13 pKa = 11.49 CFTVNNYY20 pKa = 10.67 DD21 pKa = 3.67 DD22 pKa = 5.22 DD23 pKa = 4.38 GVRR26 pKa = 11.84 LLSGLVQGGIAGYY39 pKa = 9.58 ICFGYY44 pKa = 10.62 EE45 pKa = 3.95 CGKK48 pKa = 10.8 SGTKK52 pKa = 9.89 HH53 pKa = 5.17 LQGFIQFTRR62 pKa = 11.84 KK63 pKa = 8.56 VRR65 pKa = 11.84 FSTVQASLPKK75 pKa = 9.13 GTHH78 pKa = 4.34 IEE80 pKa = 4.0 KK81 pKa = 10.49 RR82 pKa = 11.84 RR83 pKa = 11.84 GTPEE87 pKa = 3.62 EE88 pKa = 3.68 ASEE91 pKa = 4.3 YY92 pKa = 10.1 CKK94 pKa = 10.82 KK95 pKa = 10.31 EE96 pKa = 3.97 GNYY99 pKa = 9.99 HH100 pKa = 6.27 EE101 pKa = 4.78 EE102 pKa = 4.39 GKK104 pKa = 10.59 LSKK107 pKa = 10.62 SGNRR111 pKa = 11.84 SDD113 pKa = 4.49 LEE115 pKa = 4.11 AVAKK119 pKa = 10.03 EE120 pKa = 4.24 VARR123 pKa = 11.84 GASDD127 pKa = 3.29 FQIAEE132 pKa = 4.1 TFPAVFVRR140 pKa = 11.84 YY141 pKa = 9.24 YY142 pKa = 10.36 RR143 pKa = 11.84 GLRR146 pKa = 11.84 EE147 pKa = 4.16 LRR149 pKa = 11.84 CVLNGNVQRR158 pKa = 11.84 SWKK161 pKa = 7.76 TRR163 pKa = 11.84 CHH165 pKa = 6.1 VYY167 pKa = 10.2 YY168 pKa = 10.45 GYY170 pKa = 9.84 PGSGKK175 pKa = 10.1 SLQASQLGTQLSPGNVYY192 pKa = 10.42 YY193 pKa = 10.7 KK194 pKa = 10.56 PRR196 pKa = 11.84 GEE198 pKa = 3.65 WWDD201 pKa = 3.81 GYY203 pKa = 10.27 EE204 pKa = 3.87 SHH206 pKa = 6.7 EE207 pKa = 4.49 VVIIDD212 pKa = 5.49 DD213 pKa = 4.43 FYY215 pKa = 10.53 GWIVFDD221 pKa = 5.06 EE222 pKa = 4.29 LLKK225 pKa = 10.73 ICDD228 pKa = 3.7 RR229 pKa = 11.84 YY230 pKa = 10.13 PYY232 pKa = 9.96 RR233 pKa = 11.84 VPIKK237 pKa = 9.73 GGYY240 pKa = 8.82 RR241 pKa = 11.84 NFVARR246 pKa = 11.84 DD247 pKa = 3.23 IFITSNSGPDD257 pKa = 2.76 NWYY260 pKa = 8.41 PNVFEE265 pKa = 4.7 RR266 pKa = 11.84 EE267 pKa = 4.15 PIKK270 pKa = 10.72 RR271 pKa = 11.84 EE272 pKa = 3.58 AMSRR276 pKa = 11.84 RR277 pKa = 11.84 FEE279 pKa = 3.95 YY280 pKa = 10.84 VIEE283 pKa = 4.33 FKK285 pKa = 10.98 KK286 pKa = 10.02 MCDD289 pKa = 3.13 HH290 pKa = 6.87 FVCEE294 pKa = 4.35 IEE296 pKa = 4.89 KK297 pKa = 10.28 FDD299 pKa = 3.96 YY300 pKa = 10.82 EE301 pKa = 4.46 NNVYY305 pKa = 10.37 EE306 pKa = 4.56 LAII309 pKa = 4.33
Molecular weight: 35.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.207
IPC2_protein 7.512
IPC_protein 7.38
Toseland 6.985
ProMoST 7.936
Dawson 8.068
Bjellqvist 8.434
Wikipedia 7.995
Rodwell 8.083
Grimsley 7.0
Solomon 8.141
Lehninger 8.156
Nozaki 8.565
DTASelect 8.17
Thurlkill 8.214
EMBOSS 8.273
Sillero 8.536
Patrickios 3.948
IPC_peptide 8.141
IPC2_peptide 7.483
IPC2.peptide.svr19 7.607
Protein with the highest isoelectric point:
>tr|A0A346BP99|A0A346BP99_9VIRU Putative capsid protein OS=Fly associated circular virus 5 OX=2293285 PE=4 SV=1
MM1 pKa = 7.66 AFNLRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 FNRR11 pKa = 11.84 RR12 pKa = 11.84 NYY14 pKa = 9.59 FGHH17 pKa = 6.53 RR18 pKa = 11.84 RR19 pKa = 11.84 MNFRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 IHH27 pKa = 6.62 RR28 pKa = 11.84 RR29 pKa = 11.84 FAPTRR34 pKa = 11.84 SFRR37 pKa = 11.84 TNLYY41 pKa = 10.26 KK42 pKa = 10.52 KK43 pKa = 9.3 RR44 pKa = 11.84 TWKK47 pKa = 10.34 RR48 pKa = 11.84 MRR50 pKa = 11.84 ANYY53 pKa = 9.58 IKK55 pKa = 9.79 IYY57 pKa = 9.38 STGPPATLIQKK68 pKa = 10.18 RR69 pKa = 11.84 NGVSGSVHH77 pKa = 6.26 FNLAAIFPKK86 pKa = 8.57 WANVAEE92 pKa = 5.07 HH93 pKa = 6.87 IEE95 pKa = 3.97 QLRR98 pKa = 11.84 VAKK101 pKa = 10.44 VKK103 pKa = 10.73 LRR105 pKa = 11.84 FTPMFSRR112 pKa = 11.84 GGAFTEE118 pKa = 4.6 SGALLADD125 pKa = 3.21 QRR127 pKa = 11.84 VYY129 pKa = 10.39 FAYY132 pKa = 10.59 NKK134 pKa = 10.21 LSEE137 pKa = 4.18 TSEE140 pKa = 4.03 PDD142 pKa = 3.01 VRR144 pKa = 11.84 EE145 pKa = 4.17 DD146 pKa = 3.57 VVCFPKK152 pKa = 10.59 VFSKK156 pKa = 8.84 TVRR159 pKa = 11.84 QSFTWTLKK167 pKa = 9.56 PHH169 pKa = 5.68 MRR171 pKa = 11.84 QNTTVTSVADD181 pKa = 3.57 NGAEE185 pKa = 3.9 NTINVPTSIPFRR197 pKa = 11.84 YY198 pKa = 9.68 INPNDD203 pKa = 3.94 DD204 pKa = 3.71 AVSGINPIHH213 pKa = 6.44 GAQFYY218 pKa = 10.31 MDD220 pKa = 5.11 GLPALPEE227 pKa = 4.46 AYY229 pKa = 9.88 GDD231 pKa = 3.57 WGDD234 pKa = 5.41 SVPVWNITASAEE246 pKa = 3.91 IHH248 pKa = 6.41 GKK250 pKa = 8.47 GLNYY254 pKa = 9.74 IAPPGSIKK262 pKa = 10.66 KK263 pKa = 8.0 FTSDD267 pKa = 3.35 VPLIEE272 pKa = 4.78 IKK274 pKa = 11.01 YY275 pKa = 10.14 KK276 pKa = 10.91 DD277 pKa = 3.66 VII279 pKa = 3.97
Molecular weight: 32.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.303
IPC2_protein 9.765
IPC_protein 10.394
Toseland 10.54
ProMoST 10.204
Dawson 10.672
Bjellqvist 10.365
Wikipedia 10.862
Rodwell 10.994
Grimsley 10.73
Solomon 10.73
Lehninger 10.701
Nozaki 10.496
DTASelect 10.35
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.584
Patrickios 10.672
IPC_peptide 10.73
IPC2_peptide 9.121
IPC2.peptide.svr19 8.672
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
588
279
309
294.0
33.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.293 ± 1.051
1.701 ± 0.886
4.422 ± 0.316
6.122 ± 1.675
5.952 ± 0.33
7.483 ± 1.154
2.211 ± 0.197
5.782 ± 0.442
6.633 ± 0.356
5.102 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.531 ± 0.409
5.442 ± 0.903
5.272 ± 0.779
2.551 ± 0.264
8.333 ± 0.651
6.122 ± 0.019
4.762 ± 1.115
6.973 ± 0.107
1.701 ± 0.06
5.612 ± 1.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here