Fly associated circular virus 5

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A346BP99|A0A346BP99_9VIRU Putative capsid protein OS=Fly associated circular virus 5 OX=2293285 PE=4 SV=1
MM1 pKa = 7.74PKK3 pKa = 10.35AKK5 pKa = 10.05RR6 pKa = 11.84EE7 pKa = 4.23SPAKK11 pKa = 10.17DD12 pKa = 3.24YY13 pKa = 11.49CFTVNNYY20 pKa = 10.67DD21 pKa = 3.67DD22 pKa = 5.22DD23 pKa = 4.38GVRR26 pKa = 11.84LLSGLVQGGIAGYY39 pKa = 9.58ICFGYY44 pKa = 10.62EE45 pKa = 3.95CGKK48 pKa = 10.8SGTKK52 pKa = 9.89HH53 pKa = 5.17LQGFIQFTRR62 pKa = 11.84KK63 pKa = 8.56VRR65 pKa = 11.84FSTVQASLPKK75 pKa = 9.13GTHH78 pKa = 4.34IEE80 pKa = 4.0KK81 pKa = 10.49RR82 pKa = 11.84RR83 pKa = 11.84GTPEE87 pKa = 3.62EE88 pKa = 3.68ASEE91 pKa = 4.3YY92 pKa = 10.1CKK94 pKa = 10.82KK95 pKa = 10.31EE96 pKa = 3.97GNYY99 pKa = 9.99HH100 pKa = 6.27EE101 pKa = 4.78EE102 pKa = 4.39GKK104 pKa = 10.59LSKK107 pKa = 10.62SGNRR111 pKa = 11.84SDD113 pKa = 4.49LEE115 pKa = 4.11AVAKK119 pKa = 10.03EE120 pKa = 4.24VARR123 pKa = 11.84GASDD127 pKa = 3.29FQIAEE132 pKa = 4.1TFPAVFVRR140 pKa = 11.84YY141 pKa = 9.24YY142 pKa = 10.36RR143 pKa = 11.84GLRR146 pKa = 11.84EE147 pKa = 4.16LRR149 pKa = 11.84CVLNGNVQRR158 pKa = 11.84SWKK161 pKa = 7.76TRR163 pKa = 11.84CHH165 pKa = 6.1VYY167 pKa = 10.2YY168 pKa = 10.45GYY170 pKa = 9.84PGSGKK175 pKa = 10.1SLQASQLGTQLSPGNVYY192 pKa = 10.42YY193 pKa = 10.7KK194 pKa = 10.56PRR196 pKa = 11.84GEE198 pKa = 3.65WWDD201 pKa = 3.81GYY203 pKa = 10.27EE204 pKa = 3.87SHH206 pKa = 6.7EE207 pKa = 4.49VVIIDD212 pKa = 5.49DD213 pKa = 4.43FYY215 pKa = 10.53GWIVFDD221 pKa = 5.06EE222 pKa = 4.29LLKK225 pKa = 10.73ICDD228 pKa = 3.7RR229 pKa = 11.84YY230 pKa = 10.13PYY232 pKa = 9.96RR233 pKa = 11.84VPIKK237 pKa = 9.73GGYY240 pKa = 8.82RR241 pKa = 11.84NFVARR246 pKa = 11.84DD247 pKa = 3.23IFITSNSGPDD257 pKa = 2.76NWYY260 pKa = 8.41PNVFEE265 pKa = 4.7RR266 pKa = 11.84EE267 pKa = 4.15PIKK270 pKa = 10.72RR271 pKa = 11.84EE272 pKa = 3.58AMSRR276 pKa = 11.84RR277 pKa = 11.84FEE279 pKa = 3.95YY280 pKa = 10.84VIEE283 pKa = 4.33FKK285 pKa = 10.98KK286 pKa = 10.02MCDD289 pKa = 3.13HH290 pKa = 6.87FVCEE294 pKa = 4.35IEE296 pKa = 4.89KK297 pKa = 10.28FDD299 pKa = 3.96YY300 pKa = 10.82EE301 pKa = 4.46NNVYY305 pKa = 10.37EE306 pKa = 4.56LAII309 pKa = 4.33

Molecular weight:
35.71 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A346BP99|A0A346BP99_9VIRU Putative capsid protein OS=Fly associated circular virus 5 OX=2293285 PE=4 SV=1
MM1 pKa = 7.66AFNLRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84FNRR11 pKa = 11.84RR12 pKa = 11.84NYY14 pKa = 9.59FGHH17 pKa = 6.53RR18 pKa = 11.84RR19 pKa = 11.84MNFRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84IHH27 pKa = 6.62RR28 pKa = 11.84RR29 pKa = 11.84FAPTRR34 pKa = 11.84SFRR37 pKa = 11.84TNLYY41 pKa = 10.26KK42 pKa = 10.52KK43 pKa = 9.3RR44 pKa = 11.84TWKK47 pKa = 10.34RR48 pKa = 11.84MRR50 pKa = 11.84ANYY53 pKa = 9.58IKK55 pKa = 9.79IYY57 pKa = 9.38STGPPATLIQKK68 pKa = 10.18RR69 pKa = 11.84NGVSGSVHH77 pKa = 6.26FNLAAIFPKK86 pKa = 8.57WANVAEE92 pKa = 5.07HH93 pKa = 6.87IEE95 pKa = 3.97QLRR98 pKa = 11.84VAKK101 pKa = 10.44VKK103 pKa = 10.73LRR105 pKa = 11.84FTPMFSRR112 pKa = 11.84GGAFTEE118 pKa = 4.6SGALLADD125 pKa = 3.21QRR127 pKa = 11.84VYY129 pKa = 10.39FAYY132 pKa = 10.59NKK134 pKa = 10.21LSEE137 pKa = 4.18TSEE140 pKa = 4.03PDD142 pKa = 3.01VRR144 pKa = 11.84EE145 pKa = 4.17DD146 pKa = 3.57VVCFPKK152 pKa = 10.59VFSKK156 pKa = 8.84TVRR159 pKa = 11.84QSFTWTLKK167 pKa = 9.56PHH169 pKa = 5.68MRR171 pKa = 11.84QNTTVTSVADD181 pKa = 3.57NGAEE185 pKa = 3.9NTINVPTSIPFRR197 pKa = 11.84YY198 pKa = 9.68INPNDD203 pKa = 3.94DD204 pKa = 3.71AVSGINPIHH213 pKa = 6.44GAQFYY218 pKa = 10.31MDD220 pKa = 5.11GLPALPEE227 pKa = 4.46AYY229 pKa = 9.88GDD231 pKa = 3.57WGDD234 pKa = 5.41SVPVWNITASAEE246 pKa = 3.91IHH248 pKa = 6.41GKK250 pKa = 8.47GLNYY254 pKa = 9.74IAPPGSIKK262 pKa = 10.66KK263 pKa = 8.0FTSDD267 pKa = 3.35VPLIEE272 pKa = 4.78IKK274 pKa = 11.01YY275 pKa = 10.14KK276 pKa = 10.91DD277 pKa = 3.66VII279 pKa = 3.97

Molecular weight:
32.01 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

588

279

309

294.0

33.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.293 ± 1.051

1.701 ± 0.886

4.422 ± 0.316

6.122 ± 1.675

5.952 ± 0.33

7.483 ± 1.154

2.211 ± 0.197

5.782 ± 0.442

6.633 ± 0.356

5.102 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.531 ± 0.409

5.442 ± 0.903

5.272 ± 0.779

2.551 ± 0.264

8.333 ± 0.651

6.122 ± 0.019

4.762 ± 1.115

6.973 ± 0.107

1.701 ± 0.06

5.612 ± 1.102

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski