Lepus americanus faeces associated genomovirus SHP11
Average proteome isoelectric point is 7.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CJA9|A0A2Z5CJA9_9VIRU Replication-associated protein OS=Lepus americanus faeces associated genomovirus SHP11 OX=2219117 PE=4 SV=1
MM1 pKa = 7.0 VRR3 pKa = 11.84 RR4 pKa = 11.84 FKK6 pKa = 11.09 LEE8 pKa = 3.71 EE9 pKa = 3.67 VSYY12 pKa = 11.61 VLLTYY17 pKa = 7.57 PTTPDD22 pKa = 3.06 GFDD25 pKa = 3.24 PQGIIDD31 pKa = 4.06 AVVAAGAVYY40 pKa = 10.25 RR41 pKa = 11.84 LGRR44 pKa = 11.84 EE45 pKa = 3.68 LHH47 pKa = 6.88 ADD49 pKa = 4.13 GKK51 pKa = 8.99 PHH53 pKa = 5.26 YY54 pKa = 10.02 HH55 pKa = 7.07 CFVQWADD62 pKa = 3.92 PYY64 pKa = 11.14 SDD66 pKa = 4.16 PDD68 pKa = 3.39 AGGTFTVGGRR78 pKa = 11.84 RR79 pKa = 11.84 PNIKK83 pKa = 10.01 KK84 pKa = 10.52 FSANPGRR91 pKa = 11.84 RR92 pKa = 11.84 WDD94 pKa = 3.78 YY95 pKa = 10.72 VGKK98 pKa = 9.62 HH99 pKa = 5.37 AGKK102 pKa = 10.38 KK103 pKa = 8.76 EE104 pKa = 3.58 GHH106 pKa = 6.6 FIIGDD111 pKa = 3.42 QCEE114 pKa = 4.28 RR115 pKa = 11.84 PSEE118 pKa = 4.14 GDD120 pKa = 2.73 VDD122 pKa = 3.89 TRR124 pKa = 11.84 SQSDD128 pKa = 2.78 IWSEE132 pKa = 3.99 IIGCTTQEE140 pKa = 4.15 EE141 pKa = 5.17 FWSKK145 pKa = 10.51 LAALAPKK152 pKa = 10.22 QLGCNFGSLKK162 pKa = 10.8 LYY164 pKa = 10.76 VDD166 pKa = 4.13 WKK168 pKa = 10.36 YY169 pKa = 11.42 KK170 pKa = 9.0 PAQEE174 pKa = 4.7 IYY176 pKa = 7.47 EE177 pKa = 4.45 TPQGTFDD184 pKa = 3.6 VPGVLQEE191 pKa = 4.18 PKK193 pKa = 10.71 GLVLFGPTRR202 pKa = 11.84 IGKK205 pKa = 6.42 TVWARR210 pKa = 11.84 SLADD214 pKa = 3.15 HH215 pKa = 7.22 AYY217 pKa = 10.45 FGGLFNLDD225 pKa = 3.99 DD226 pKa = 4.71 FSADD230 pKa = 3.03 GAQYY234 pKa = 11.09 AIFDD238 pKa = 5.15 DD239 pKa = 3.83 ISGGFSFFPSYY250 pKa = 9.2 KK251 pKa = 9.26 QWMGGQYY258 pKa = 10.39 QFTVTDD264 pKa = 3.91 KK265 pKa = 10.76 YY266 pKa = 10.28 KK267 pKa = 10.97 HH268 pKa = 5.95 KK269 pKa = 10.37 VTLRR273 pKa = 11.84 WGRR276 pKa = 11.84 PTIWLCNTDD285 pKa = 3.49 PRR287 pKa = 11.84 EE288 pKa = 3.99 DD289 pKa = 3.6 HH290 pKa = 6.54 YY291 pKa = 11.83 KK292 pKa = 10.08 PGAMPDD298 pKa = 3.87 FAWMEE303 pKa = 4.29 EE304 pKa = 3.69 NCVFYY309 pKa = 10.62 EE310 pKa = 4.02 CRR312 pKa = 11.84 DD313 pKa = 4.1 TIFHH317 pKa = 7.24 ASTT320 pKa = 3.35
Molecular weight: 36.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.56
IPC2_protein 5.575
IPC_protein 5.601
Toseland 5.957
ProMoST 5.931
Dawson 5.842
Bjellqvist 5.868
Wikipedia 5.842
Rodwell 5.817
Grimsley 6.084
Solomon 5.842
Lehninger 5.83
Nozaki 6.097
DTASelect 6.287
Thurlkill 6.275
EMBOSS 6.237
Sillero 6.198
Patrickios 3.846
IPC_peptide 5.855
IPC2_peptide 6.186
IPC2.peptide.svr19 6.185
Protein with the highest isoelectric point:
>tr|A0A2Z5CJA9|A0A2Z5CJA9_9VIRU Replication-associated protein OS=Lepus americanus faeces associated genomovirus SHP11 OX=2219117 PE=4 SV=1
MM1 pKa = 6.86 AQRR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 8.39 FTSYY10 pKa = 9.1 RR11 pKa = 11.84 AKK13 pKa = 10.33 RR14 pKa = 11.84 RR15 pKa = 11.84 TRR17 pKa = 11.84 RR18 pKa = 11.84 SARR21 pKa = 11.84 PAKK24 pKa = 9.93 YY25 pKa = 9.96 GGRR28 pKa = 11.84 KK29 pKa = 9.1 RR30 pKa = 11.84 RR31 pKa = 11.84 WTKK34 pKa = 7.47 TRR36 pKa = 11.84 KK37 pKa = 7.67 TSRR40 pKa = 11.84 PMTKK44 pKa = 10.02 KK45 pKa = 10.47 RR46 pKa = 11.84 ILNTTSRR53 pKa = 11.84 KK54 pKa = 9.22 KK55 pKa = 10.55 RR56 pKa = 11.84 NGMLTWSNTNLNGTVRR72 pKa = 11.84 PIASGNAYY80 pKa = 9.56 VAATNVGVFVYY91 pKa = 10.58 CPTALHH97 pKa = 6.86 LDD99 pKa = 3.81 GDD101 pKa = 4.32 SLLRR105 pKa = 11.84 NPAARR110 pKa = 11.84 TSSTIFAKK118 pKa = 10.6 GLSEE122 pKa = 4.2 RR123 pKa = 11.84 MRR125 pKa = 11.84 IQTSSGLPWFHH136 pKa = 6.89 RR137 pKa = 11.84 RR138 pKa = 11.84 VCFTTKK144 pKa = 10.27 GPNAFNVINSTDD156 pKa = 3.46 TPTVPWAPYY165 pKa = 10.13 LDD167 pKa = 3.52 TSNGIEE173 pKa = 3.69 RR174 pKa = 11.84 PMINLDD180 pKa = 3.28 INAMPGTLGSQYY192 pKa = 11.63 GLLFKK197 pKa = 10.71 GRR199 pKa = 11.84 QGVDD203 pKa = 2.71 WNDD206 pKa = 3.71 TIIAPLDD213 pKa = 3.57 TARR216 pKa = 11.84 VTVKK220 pKa = 10.55 YY221 pKa = 10.73 DD222 pKa = 3.46 KK223 pKa = 10.63 TWTLQSGNANGVIRR237 pKa = 11.84 EE238 pKa = 4.08 RR239 pKa = 11.84 KK240 pKa = 8.44 LWHH243 pKa = 6.47 PMNHH247 pKa = 4.99 NVVYY251 pKa = 10.82 DD252 pKa = 3.82 EE253 pKa = 5.76 DD254 pKa = 4.24 EE255 pKa = 4.88 DD256 pKa = 5.73 GEE258 pKa = 4.69 VQGTSYY264 pKa = 11.49 YY265 pKa = 11.06 SVDD268 pKa = 3.46 SKK270 pKa = 11.82 AGMGDD275 pKa = 3.89 YY276 pKa = 10.83 YY277 pKa = 11.6 VVDD280 pKa = 4.56 FFQAGVGGTSTDD292 pKa = 4.19 LISVNASSTMYY303 pKa = 9.27 WHH305 pKa = 7.08 EE306 pKa = 4.08 KK307 pKa = 9.5
Molecular weight: 34.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 9.706
IPC_protein 10.233
Toseland 10.379
ProMoST 10.101
Dawson 10.54
Bjellqvist 10.233
Wikipedia 10.73
Rodwell 10.877
Grimsley 10.613
Solomon 10.584
Lehninger 10.555
Nozaki 10.35
DTASelect 10.233
Thurlkill 10.409
EMBOSS 10.774
Sillero 10.467
Patrickios 10.511
IPC_peptide 10.599
IPC2_peptide 9.033
IPC2.peptide.svr19 8.695
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
627
307
320
313.5
35.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.858 ± 0.013
1.435 ± 0.577
6.539 ± 0.977
3.668 ± 1.021
4.785 ± 1.363
9.091 ± 0.697
2.233 ± 0.445
4.147 ± 0.175
5.901 ± 0.028
5.742 ± 0.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.073 ± 0.631
4.147 ± 1.742
5.423 ± 0.634
3.19 ± 0.669
7.018 ± 1.547
6.061 ± 1.053
8.134 ± 1.684
6.061 ± 0.334
2.871 ± 0.195
4.625 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here