Bacillus sp. DSL-17

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4971 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S4BN23|A0A4S4BN23_9BACI MmgE/PrpD family protein OS=Bacillus sp. DSL-17 OX=2567941 GN=E6W99_23100 PE=3 SV=1
MM1 pKa = 8.17DD2 pKa = 4.5FQTTSGAFDD11 pKa = 3.63TVHH14 pKa = 6.81NGGFYY19 pKa = 10.82DD20 pKa = 4.75AFVTKK25 pKa = 10.6LNPAGSALVYY35 pKa = 9.44STYY38 pKa = 11.0LGGTGFDD45 pKa = 3.27QATGIAVDD53 pKa = 3.59AAGNAYY59 pKa = 8.23VTGFTDD65 pKa = 4.6SMDD68 pKa = 3.54FQTTSGAFDD77 pKa = 3.24TTYY80 pKa = 11.27NGDD83 pKa = 3.63PFDD86 pKa = 5.09AFVTKK91 pKa = 10.79LNPTGSALVYY101 pKa = 9.04STYY104 pKa = 10.99LGGEE108 pKa = 4.26LSDD111 pKa = 3.5QGNGISVDD119 pKa = 3.52AAGNAYY125 pKa = 8.21VTGLTTSRR133 pKa = 11.84DD134 pKa = 3.54FPTTQGAFDD143 pKa = 3.47TTYY146 pKa = 11.2NGGPFDD152 pKa = 5.0AFVTKK157 pKa = 10.79LNPTGSALVYY167 pKa = 9.22STYY170 pKa = 10.97LGGSSDD176 pKa = 4.54DD177 pKa = 3.42QGTDD181 pKa = 2.43IFVDD185 pKa = 3.67AEE187 pKa = 3.99GNAYY191 pKa = 8.08VTGFTQSPDD200 pKa = 3.17FPTTSGAFDD209 pKa = 3.34TTFNGGSDD217 pKa = 3.48AFVTKK222 pKa = 10.74LNSTGSAPLLYY233 pKa = 9.47STYY236 pKa = 10.85LGGNTGEE243 pKa = 4.07QAFAIAVDD251 pKa = 3.68AAGNAYY257 pKa = 8.29VTGFTISEE265 pKa = 4.52DD266 pKa = 3.91YY267 pKa = 7.79PTTSGAFDD275 pKa = 3.23TSYY278 pKa = 11.56NGFSDD283 pKa = 4.21AFVAKK288 pKa = 10.28ISTVPSTTPMPIANVSPTSINFGNQPVNTSSSSQTVTVTNTGTADD333 pKa = 4.61LIINNVMIAGEE344 pKa = 4.45NPTDD348 pKa = 3.89FQVVSNMCTIVNPGNSCTIQVTFNPTTPGTRR379 pKa = 11.84SATLNIFDD387 pKa = 4.81NVSDD391 pKa = 4.14SPQQVLLNGNGLPSADD407 pKa = 5.11LMITKK412 pKa = 10.04NAFVVPAGRR421 pKa = 11.84NRR423 pKa = 11.84KK424 pKa = 9.05QITYY428 pKa = 8.81TITVLNMGPNTASNVVVTDD447 pKa = 4.31RR448 pKa = 11.84LQSNTDD454 pKa = 3.75FVSATTSQGTFTAPRR469 pKa = 11.84RR470 pKa = 11.84GRR472 pKa = 11.84SGTVTFTLGNISAGSSANMTIVVTAKK498 pKa = 10.73ANAFVTNTATVSSSTFDD515 pKa = 3.88PGPHH519 pKa = 6.78PNSASVIIDD528 pKa = 3.36STAGNLNDD536 pKa = 3.91TGSDD540 pKa = 3.43EE541 pKa = 5.08SSGDD545 pKa = 3.26HH546 pKa = 6.56HH547 pKa = 7.2

Molecular weight: 56.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S4BU26|A0A4S4BU26_9BACI GIY-YIG nuclease family protein OS=Bacillus sp. DSL-17 OX=2567941 GN=E6W99_17310 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.48VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.94VLSAA44 pKa = 4.11

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4971

0

4971

1439422

23

2279

289.6

32.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.672 ± 0.033

0.731 ± 0.01

4.949 ± 0.03

7.366 ± 0.043

4.624 ± 0.031

6.799 ± 0.033

2.131 ± 0.019

8.474 ± 0.043

7.085 ± 0.035

9.812 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.729 ± 0.015

4.727 ± 0.026

3.521 ± 0.02

3.71 ± 0.023

3.687 ± 0.027

6.139 ± 0.025

5.485 ± 0.025

6.91 ± 0.03

0.984 ± 0.014

3.466 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski