Xanthomonas phage Suba
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A679KGF4|A0A679KGF4_9CAUD Phage Major Tail tube protein OS=Xanthomonas phage Suba OX=2674975 PE=4 SV=1
MM1 pKa = 7.56 TDD3 pKa = 3.18 KK4 pKa = 10.71 ILYY7 pKa = 6.6 FTAGRR12 pKa = 11.84 IPTTAEE18 pKa = 3.77 KK19 pKa = 11.16 AEE21 pKa = 4.02 IAAIEE26 pKa = 4.09 ALCVVPYY33 pKa = 10.36 SVGVRR38 pKa = 11.84 NPIDD42 pKa = 3.54 SPNYY46 pKa = 6.8 GTPEE50 pKa = 3.75 EE51 pKa = 4.1 ADD53 pKa = 3.67 YY54 pKa = 11.61 VAGTVPEE61 pKa = 4.16 EE62 pKa = 3.92 EE63 pKa = 5.2 PYY65 pKa = 11.32 ASLPEE70 pKa = 4.45 FDD72 pKa = 5.11 PADD75 pKa = 4.18 PPNPSLPADD84 pKa = 3.61 KK85 pKa = 10.81 AIVGNGDD92 pKa = 3.66 SYY94 pKa = 11.45 PVTGGTVSVAVAGGVPTFTFTPTPP118 pKa = 3.65
Molecular weight: 12.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.923
IPC_protein 3.821
Toseland 3.63
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.656
Grimsley 3.554
Solomon 3.77
Lehninger 3.732
Nozaki 3.923
DTASelect 4.075
Thurlkill 3.681
EMBOSS 3.719
Sillero 3.935
Patrickios 0.769
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.838
Protein with the highest isoelectric point:
>tr|A0A679KD76|A0A679KD76_9CAUD Uncharacterized protein OS=Xanthomonas phage Suba OX=2674975 PE=4 SV=1
MM1 pKa = 7.33 QSFKK5 pKa = 10.89 FRR7 pKa = 11.84 LSKK10 pKa = 10.22 IARR13 pKa = 11.84 TVSTMDD19 pKa = 3.21 LRR21 pKa = 11.84 KK22 pKa = 7.94 TAEE25 pKa = 3.72 QQRR28 pKa = 11.84 AEE30 pKa = 4.4 EE31 pKa = 4.28 IATKK35 pKa = 10.39 ARR37 pKa = 11.84 RR38 pKa = 11.84 FEE40 pKa = 4.35 SRR42 pKa = 11.84 NKK44 pKa = 8.8 TAGKK48 pKa = 9.84 RR49 pKa = 11.84 SSFPVSTEE57 pKa = 3.5 WEE59 pKa = 4.29 LVV61 pKa = 3.33
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.285
IPC2_protein 9.648
IPC_protein 10.423
Toseland 11.155
ProMoST 11.082
Dawson 11.184
Bjellqvist 10.935
Wikipedia 11.447
Rodwell 11.374
Grimsley 11.199
Solomon 11.433
Lehninger 11.389
Nozaki 11.125
DTASelect 10.935
Thurlkill 11.125
EMBOSS 11.579
Sillero 11.125
Patrickios 11.14
IPC_peptide 11.447
IPC2_peptide 9.78
IPC2.peptide.svr19 9.031
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
60
0
60
11841
37
1211
197.3
21.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.464 ± 0.954
1.056 ± 0.158
5.498 ± 0.193
7.195 ± 0.251
4.079 ± 0.314
8.183 ± 0.443
1.529 ± 0.215
5.43 ± 0.239
6.19 ± 0.315
7.17 ± 0.29
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.348 ± 0.185
4.94 ± 0.292
4.341 ± 0.396
3.876 ± 0.494
5.278 ± 0.226
5.912 ± 0.25
5.43 ± 0.308
6.672 ± 0.293
1.41 ± 0.176
2.998 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here