Bacillus sp. B-jedd

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4594 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A098EZG1|A0A098EZG1_9BACI Branched-chain alpha-keto acid dehydrogenase E1 subunit OS=Bacillus sp. B-jedd OX=1476857 GN=bkdAB PE=4 SV=1
MM1 pKa = 7.71QILLNGNDD9 pKa = 3.65EE10 pKa = 4.28FEE12 pKa = 4.73KK13 pKa = 10.95GISGQVYY20 pKa = 10.23LNVEE24 pKa = 4.49SRR26 pKa = 11.84WCLYY30 pKa = 10.53DD31 pKa = 3.46QLPNEE36 pKa = 4.39YY37 pKa = 9.52PVDD40 pKa = 4.16DD41 pKa = 4.25SDD43 pKa = 4.55IEE45 pKa = 4.13EE46 pKa = 4.45LEE48 pKa = 4.0RR49 pKa = 11.84LQEE52 pKa = 4.35LEE54 pKa = 3.88ILNDD58 pKa = 3.77LSYY61 pKa = 11.73VEE63 pKa = 4.45IVDD66 pKa = 3.91VKK68 pKa = 10.99LDD70 pKa = 3.38NSFPHH75 pKa = 7.56LILTFQNGKK84 pKa = 9.16SLFIHH89 pKa = 6.43GHH91 pKa = 5.21DD92 pKa = 3.58EE93 pKa = 4.72QYY95 pKa = 11.16EE96 pKa = 4.23SWQMGVSFQEE106 pKa = 4.48GEE108 pKa = 3.93SWLVVACPGDD118 pKa = 4.67EE119 pKa = 3.81IAIWHH124 pKa = 6.9PEE126 pKa = 3.84HH127 pKa = 7.03FSPP130 pKa = 5.85

Molecular weight: 15.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A098EW69|A0A098EW69_9BACI ATPase AAA-2 domain-containing protein OS=Bacillus sp. B-jedd OX=1476857 GN=BN1002_01469 PE=3 SV=1
MM1 pKa = 7.46IGLRR5 pKa = 11.84LTILILLLIFSVFINLLGLMKK26 pKa = 10.25IFPLLFSAPLLFLSIFFLISTLNQRR51 pKa = 11.84NRR53 pKa = 11.84FRR55 pKa = 11.84GFPPRR60 pKa = 3.71

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4594

0

4594

1315136

29

3048

286.3

31.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.71 ± 0.041

0.715 ± 0.013

4.983 ± 0.03

7.489 ± 0.049

4.698 ± 0.037

7.499 ± 0.043

1.891 ± 0.015

7.474 ± 0.039

7.007 ± 0.037

9.768 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.783 ± 0.02

4.248 ± 0.028

3.784 ± 0.025

3.241 ± 0.024

4.3 ± 0.03

5.932 ± 0.024

5.158 ± 0.032

6.8 ± 0.028

1.053 ± 0.014

3.469 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski