Orchid fleck dichorhavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Dichorhavirus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1MWA2|Q1MWA2_9RHAB Nucleocapsid protein OS=Orchid fleck dichorhavirus OX=152177 GN=N PE=4 SV=1
MM1 pKa = 7.79ANPSEE6 pKa = 4.36IDD8 pKa = 3.35YY9 pKa = 7.46MTPLSAYY16 pKa = 8.51EE17 pKa = 4.13GVPAEE22 pKa = 4.14YY23 pKa = 10.45QEE25 pKa = 4.69ATSSPTPKK33 pKa = 10.37EE34 pKa = 4.06YY35 pKa = 10.43TRR37 pKa = 11.84DD38 pKa = 3.34AAKK41 pKa = 10.53AIPICILPAPPGNEE55 pKa = 3.77VEE57 pKa = 4.22VAEE60 pKa = 4.65AFRR63 pKa = 11.84EE64 pKa = 4.44ATQGTEE70 pKa = 4.01TVLSKK75 pKa = 11.05LQLAQIMSLGFMIQMSGDD93 pKa = 3.79PEE95 pKa = 4.89ALMQGVAEE103 pKa = 4.63GSFLEE108 pKa = 4.7TMTMPDD114 pKa = 2.47IKK116 pKa = 9.92TRR118 pKa = 11.84QDD120 pKa = 3.09SRR122 pKa = 11.84ITAAIAAMALDD133 pKa = 4.13PTEE136 pKa = 4.8EE137 pKa = 4.71GPDD140 pKa = 3.7DD141 pKa = 5.16LEE143 pKa = 5.82DD144 pKa = 4.36RR145 pKa = 11.84SQQADD150 pKa = 3.53AEE152 pKa = 4.59SAMSKK157 pKa = 10.06ARR159 pKa = 11.84AMVYY163 pKa = 9.67ICLSLMRR170 pKa = 11.84LAVKK174 pKa = 9.51PAEE177 pKa = 4.1SFMKK181 pKa = 10.34GVHH184 pKa = 5.75QIKK187 pKa = 9.37QAYY190 pKa = 7.8SVLVGEE196 pKa = 4.56HH197 pKa = 6.54SEE199 pKa = 4.08FLFNYY204 pKa = 9.5SYY206 pKa = 11.55SEE208 pKa = 4.14GMCRR212 pKa = 11.84NIADD216 pKa = 4.46MFNQCDD222 pKa = 3.85DD223 pKa = 4.2LKK225 pKa = 11.07ATLCHH230 pKa = 6.27HH231 pKa = 6.74CAIADD236 pKa = 3.98EE237 pKa = 4.42THH239 pKa = 5.1HH240 pKa = 6.13TNRR243 pKa = 11.84KK244 pKa = 7.81RR245 pKa = 11.84HH246 pKa = 4.7GLLRR250 pKa = 11.84FLILQHH256 pKa = 6.55VDD258 pKa = 3.28LTGMIPYY265 pKa = 10.6GMYY268 pKa = 9.6IDD270 pKa = 3.44MRR272 pKa = 11.84RR273 pKa = 11.84YY274 pKa = 7.85FTLLTPGQLLTWLHH288 pKa = 6.97DD289 pKa = 4.03NQVSRR294 pKa = 11.84PLSVIADD301 pKa = 3.12INTRR305 pKa = 11.84YY306 pKa = 10.11DD307 pKa = 3.19VSNGSDD313 pKa = 3.36RR314 pKa = 11.84FWRR317 pKa = 11.84YY318 pKa = 9.84SRR320 pKa = 11.84GLDD323 pKa = 3.06PGFFIALQQSKK334 pKa = 10.26CVTLIARR341 pKa = 11.84MAHH344 pKa = 6.0ILVKK348 pKa = 10.7GGAVAVNEE356 pKa = 4.04YY357 pKa = 10.9SDD359 pKa = 3.61PRR361 pKa = 11.84KK362 pKa = 10.32AKK364 pKa = 10.46SLEE367 pKa = 4.11NKK369 pKa = 9.62PGLAAEE375 pKa = 4.36ADD377 pKa = 3.53KK378 pKa = 10.78FATEE382 pKa = 4.34FVEE385 pKa = 5.32AYY387 pKa = 9.89NGLSGSSANAGPVSRR402 pKa = 11.84KK403 pKa = 9.75LYY405 pKa = 8.68NQGRR409 pKa = 11.84GIPTRR414 pKa = 11.84RR415 pKa = 11.84GLFTPPSARR424 pKa = 11.84PAPVVNVHH432 pKa = 5.68VPAASSSLTGALDD445 pKa = 4.39AMNSDD450 pKa = 3.92

Molecular weight: 49.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1MWA0|Q1MWA0_9RHAB ORF3 protein OS=Orchid fleck dichorhavirus OX=152177 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 10.18QINMCTVMSFRR14 pKa = 11.84SLITVIGPRR23 pKa = 11.84SVDD26 pKa = 3.07LGAGFKK32 pKa = 10.28EE33 pKa = 4.01AVLRR37 pKa = 11.84AITSLKK43 pKa = 10.6VKK45 pKa = 10.77AKK47 pKa = 9.94DD48 pKa = 3.5PKK50 pKa = 10.41GAEE53 pKa = 3.87IDD55 pKa = 4.4GPWQEE60 pKa = 4.66LLVEE64 pKa = 4.58IATMTKK70 pKa = 10.21SAFTGPTIKK79 pKa = 10.23KK80 pKa = 10.02DD81 pKa = 3.55LEE83 pKa = 3.95QGNVYY88 pKa = 9.87RR89 pKa = 11.84YY90 pKa = 10.27AMTVGGMLSTSHH102 pKa = 5.66NTNGRR107 pKa = 11.84LMTVRR112 pKa = 11.84TGPLYY117 pKa = 10.29DD118 pKa = 3.45TDD120 pKa = 4.25HH121 pKa = 6.27YY122 pKa = 10.69HH123 pKa = 6.69VSGEE127 pKa = 3.9VDD129 pKa = 3.03IDD131 pKa = 3.66KK132 pKa = 10.47MDD134 pKa = 3.72GFKK137 pKa = 10.38ATITLSVATVKK148 pKa = 10.53RR149 pKa = 11.84EE150 pKa = 3.89RR151 pKa = 11.84QQCEE155 pKa = 3.53AGIGEE160 pKa = 4.34YY161 pKa = 10.08HH162 pKa = 6.5VLPIKK167 pKa = 10.56GKK169 pKa = 9.4NPPRR173 pKa = 11.84SASNTAGPSGG183 pKa = 3.38

Molecular weight: 19.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3624

183

1877

604.0

67.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.015 ± 1.206

2.07 ± 0.265

5.464 ± 0.412

5.602 ± 0.276

2.98 ± 0.284

6.485 ± 0.284

2.925 ± 0.41

6.319 ± 0.49

5.85 ± 0.449

8.609 ± 0.486

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.305 ± 0.36

4.222 ± 0.166

4.691 ± 0.59

2.732 ± 0.324

5.16 ± 0.538

8.223 ± 0.278

6.705 ± 0.769

6.843 ± 0.524

1.738 ± 0.329

3.063 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski