Amino acid dipepetide frequency for Streptococcus phage IPP62

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.076AlaAla: 3.076 ± 1.097
0.473AlaCys: 0.473 ± 0.258
4.89AlaAsp: 4.89 ± 0.697
5.678AlaGlu: 5.678 ± 1.027
2.603AlaPhe: 2.603 ± 0.488
5.599AlaGly: 5.599 ± 1.117
0.71AlaHis: 0.71 ± 0.208
4.574AlaIle: 4.574 ± 0.703
5.994AlaLys: 5.994 ± 1.203
7.492AlaLeu: 7.492 ± 0.943
2.681AlaMet: 2.681 ± 0.595
4.259AlaAsn: 4.259 ± 0.962
1.498AlaPro: 1.498 ± 0.373
2.208AlaGln: 2.208 ± 0.547
4.022AlaArg: 4.022 ± 0.608
5.047AlaSer: 5.047 ± 1.198
4.89AlaThr: 4.89 ± 0.869
3.312AlaVal: 3.312 ± 0.573
0.946AlaTrp: 0.946 ± 0.256
1.498AlaTyr: 1.498 ± 0.447
0.0AlaXaa: 0.0 ± 0.0
Cys
0.237CysAla: 0.237 ± 0.123
0.079CysCys: 0.079 ± 0.115
0.237CysAsp: 0.237 ± 0.132
0.631CysGlu: 0.631 ± 0.298
0.158CysPhe: 0.158 ± 0.096
0.237CysGly: 0.237 ± 0.213
0.158CysHis: 0.158 ± 0.099
0.394CysIle: 0.394 ± 0.259
0.394CysLys: 0.394 ± 0.259
0.631CysLeu: 0.631 ± 0.314
0.079CysMet: 0.079 ± 0.086
0.079CysAsn: 0.079 ± 0.086
0.237CysPro: 0.237 ± 0.191
0.079CysGln: 0.079 ± 0.106
0.473CysArg: 0.473 ± 0.238
0.394CysSer: 0.394 ± 0.277
0.237CysThr: 0.237 ± 0.161
0.394CysVal: 0.394 ± 0.22
0.315CysTrp: 0.315 ± 0.168
0.315CysTyr: 0.315 ± 0.137
0.0CysXaa: 0.0 ± 0.0
Asp
4.416AspAla: 4.416 ± 0.755
0.315AspCys: 0.315 ± 0.226
3.312AspAsp: 3.312 ± 1.123
4.101AspGlu: 4.101 ± 0.866
2.918AspPhe: 2.918 ± 0.508
4.338AspGly: 4.338 ± 0.814
1.183AspHis: 1.183 ± 0.42
4.259AspIle: 4.259 ± 0.547
5.442AspLys: 5.442 ± 0.811
4.653AspLeu: 4.653 ± 0.679
1.498AspMet: 1.498 ± 0.376
2.681AspAsn: 2.681 ± 0.395
1.183AspPro: 1.183 ± 0.343
1.656AspGln: 1.656 ± 0.474
1.972AspArg: 1.972 ± 0.452
2.918AspSer: 2.918 ± 0.442
2.287AspThr: 2.287 ± 0.49
5.047AspVal: 5.047 ± 0.632
1.183AspTrp: 1.183 ± 0.314
3.155AspTyr: 3.155 ± 0.78
0.0AspXaa: 0.0 ± 0.0
Glu
6.388GluAla: 6.388 ± 1.021
0.552GluCys: 0.552 ± 0.26
4.653GluAsp: 4.653 ± 0.736
6.546GluGlu: 6.546 ± 0.855
4.101GluPhe: 4.101 ± 0.736
3.785GluGly: 3.785 ± 0.611
0.158GluHis: 0.158 ± 0.143
6.073GluIle: 6.073 ± 0.545
7.808GluLys: 7.808 ± 1.44
8.202GluLeu: 8.202 ± 1.067
2.05GluMet: 2.05 ± 0.543
5.442GluAsn: 5.442 ± 0.808
1.577GluPro: 1.577 ± 0.612
3.233GluGln: 3.233 ± 0.925
3.233GluArg: 3.233 ± 0.544
4.574GluSer: 4.574 ± 0.63
3.549GluThr: 3.549 ± 0.585
4.89GluVal: 4.89 ± 0.552
1.262GluTrp: 1.262 ± 0.37
2.287GluTyr: 2.287 ± 0.473
0.0GluXaa: 0.0 ± 0.0
Phe
2.76PheAla: 2.76 ± 0.843
0.394PheCys: 0.394 ± 0.218
3.549PheAsp: 3.549 ± 0.52
3.628PheGlu: 3.628 ± 0.486
1.498PhePhe: 1.498 ± 0.516
2.524PheGly: 2.524 ± 0.522
0.315PheHis: 0.315 ± 0.19
3.155PheIle: 3.155 ± 0.77
3.47PheLys: 3.47 ± 0.664
3.628PheLeu: 3.628 ± 0.834
1.183PheMet: 1.183 ± 0.463
2.445PheAsn: 2.445 ± 0.458
1.104PhePro: 1.104 ± 0.356
1.341PheGln: 1.341 ± 0.297
2.05PheArg: 2.05 ± 0.376
2.366PheSer: 2.366 ± 0.465
2.839PheThr: 2.839 ± 0.36
1.498PheVal: 1.498 ± 0.562
0.473PheTrp: 0.473 ± 0.187
2.839PheTyr: 2.839 ± 0.6
0.0PheXaa: 0.0 ± 0.0
Gly
3.785GlyAla: 3.785 ± 0.923
0.237GlyCys: 0.237 ± 0.115
3.233GlyAsp: 3.233 ± 0.56
3.785GlyGlu: 3.785 ± 0.622
4.338GlyPhe: 4.338 ± 0.809
4.968GlyGly: 4.968 ± 0.548
0.946GlyHis: 0.946 ± 0.323
4.101GlyIle: 4.101 ± 0.469
4.653GlyLys: 4.653 ± 0.545
6.546GlyLeu: 6.546 ± 0.79
1.814GlyMet: 1.814 ± 0.495
3.864GlyAsn: 3.864 ± 0.53
0.868GlyPro: 0.868 ± 0.22
3.312GlyGln: 3.312 ± 0.627
3.943GlyArg: 3.943 ± 0.64
3.943GlySer: 3.943 ± 0.747
3.312GlyThr: 3.312 ± 0.636
4.101GlyVal: 4.101 ± 0.53
1.025GlyTrp: 1.025 ± 0.455
2.603GlyTyr: 2.603 ± 0.408
0.0GlyXaa: 0.0 ± 0.0
His
0.71HisAla: 0.71 ± 0.327
0.158HisCys: 0.158 ± 0.146
0.631HisAsp: 0.631 ± 0.313
1.183HisGlu: 1.183 ± 0.368
0.868HisPhe: 0.868 ± 0.258
1.025HisGly: 1.025 ± 0.214
0.237HisHis: 0.237 ± 0.139
1.025HisIle: 1.025 ± 0.38
0.946HisLys: 0.946 ± 0.257
1.341HisLeu: 1.341 ± 0.398
0.158HisMet: 0.158 ± 0.132
0.789HisAsn: 0.789 ± 0.197
0.552HisPro: 0.552 ± 0.146
0.868HisGln: 0.868 ± 0.291
0.079HisArg: 0.079 ± 0.053
1.262HisSer: 1.262 ± 0.528
0.71HisThr: 0.71 ± 0.269
0.868HisVal: 0.868 ± 0.359
0.0HisTrp: 0.0 ± 0.0
0.552HisTyr: 0.552 ± 0.229
0.0HisXaa: 0.0 ± 0.0
Ile
4.732IleAla: 4.732 ± 0.802
0.71IleCys: 0.71 ± 0.237
3.943IleAsp: 3.943 ± 0.624
6.467IleGlu: 6.467 ± 1.019
2.287IlePhe: 2.287 ± 0.45
4.416IleGly: 4.416 ± 0.762
0.868IleHis: 0.868 ± 0.316
3.943IleIle: 3.943 ± 0.916
5.678IleLys: 5.678 ± 1.042
5.047IleLeu: 5.047 ± 0.735
0.946IleMet: 0.946 ± 0.292
3.076IleAsn: 3.076 ± 0.507
1.735IlePro: 1.735 ± 0.378
1.972IleGln: 1.972 ± 0.421
4.101IleArg: 4.101 ± 0.826
4.89IleSer: 4.89 ± 1.304
2.997IleThr: 2.997 ± 0.421
3.155IleVal: 3.155 ± 0.577
0.946IleTrp: 0.946 ± 0.275
1.814IleTyr: 1.814 ± 0.539
0.0IleXaa: 0.0 ± 0.0
Lys
6.309LysAla: 6.309 ± 0.933
0.394LysCys: 0.394 ± 0.266
5.047LysAsp: 5.047 ± 0.613
7.177LysGlu: 7.177 ± 1.417
2.681LysPhe: 2.681 ± 0.542
4.968LysGly: 4.968 ± 0.766
1.42LysHis: 1.42 ± 0.378
4.811LysIle: 4.811 ± 0.713
5.915LysLys: 5.915 ± 0.92
7.413LysLeu: 7.413 ± 0.622
1.656LysMet: 1.656 ± 0.283
4.89LysAsn: 4.89 ± 0.476
1.972LysPro: 1.972 ± 0.475
4.89LysGln: 4.89 ± 0.613
4.495LysArg: 4.495 ± 0.817
5.599LysSer: 5.599 ± 0.73
4.101LysThr: 4.101 ± 0.559
4.968LysVal: 4.968 ± 0.505
0.946LysTrp: 0.946 ± 0.221
2.366LysTyr: 2.366 ± 0.36
0.0LysXaa: 0.0 ± 0.0
Leu
6.861LeuAla: 6.861 ± 0.968
0.158LeuCys: 0.158 ± 0.099
5.126LeuAsp: 5.126 ± 0.504
7.492LeuGlu: 7.492 ± 0.606
3.391LeuPhe: 3.391 ± 0.749
5.915LeuGly: 5.915 ± 0.493
1.262LeuHis: 1.262 ± 0.376
4.495LeuIle: 4.495 ± 0.704
6.861LeuLys: 6.861 ± 0.792
7.808LeuLeu: 7.808 ± 1.257
1.893LeuMet: 1.893 ± 0.592
6.151LeuAsn: 6.151 ± 0.824
2.129LeuPro: 2.129 ± 0.592
2.366LeuGln: 2.366 ± 0.694
4.732LeuArg: 4.732 ± 0.447
7.177LeuSer: 7.177 ± 1.228
5.521LeuThr: 5.521 ± 0.723
4.732LeuVal: 4.732 ± 0.659
0.237LeuTrp: 0.237 ± 0.122
2.997LeuTyr: 2.997 ± 0.639
0.0LeuXaa: 0.0 ± 0.0
Met
1.656MetAla: 1.656 ± 0.35
0.158MetCys: 0.158 ± 0.094
1.341MetAsp: 1.341 ± 0.399
2.208MetGlu: 2.208 ± 0.487
0.631MetPhe: 0.631 ± 0.309
1.341MetGly: 1.341 ± 0.328
0.315MetHis: 0.315 ± 0.225
1.814MetIle: 1.814 ± 0.474
2.208MetLys: 2.208 ± 0.653
1.656MetLeu: 1.656 ± 0.361
0.789MetMet: 0.789 ± 0.257
1.42MetAsn: 1.42 ± 0.503
0.789MetPro: 0.789 ± 0.198
0.789MetGln: 0.789 ± 0.305
0.946MetArg: 0.946 ± 0.347
1.656MetSer: 1.656 ± 0.43
1.735MetThr: 1.735 ± 0.46
1.656MetVal: 1.656 ± 0.42
0.0MetTrp: 0.0 ± 0.0
0.631MetTyr: 0.631 ± 0.236
0.0MetXaa: 0.0 ± 0.0
Asn
4.811AsnAla: 4.811 ± 1.136
0.315AsnCys: 0.315 ± 0.256
2.603AsnAsp: 2.603 ± 0.485
3.707AsnGlu: 3.707 ± 0.536
2.681AsnPhe: 2.681 ± 0.423
4.101AsnGly: 4.101 ± 0.587
1.42AsnHis: 1.42 ± 0.456
3.864AsnIle: 3.864 ± 0.582
3.785AsnLys: 3.785 ± 0.529
4.495AsnLeu: 4.495 ± 0.759
0.789AsnMet: 0.789 ± 0.306
2.76AsnAsn: 2.76 ± 0.562
2.287AsnPro: 2.287 ± 0.365
3.47AsnGln: 3.47 ± 0.784
2.208AsnArg: 2.208 ± 0.466
4.416AsnSer: 4.416 ± 0.841
3.233AsnThr: 3.233 ± 0.381
4.101AsnVal: 4.101 ± 0.755
0.946AsnTrp: 0.946 ± 0.267
1.341AsnTyr: 1.341 ± 0.362
0.0AsnXaa: 0.0 ± 0.0
Pro
1.577ProAla: 1.577 ± 0.369
0.158ProCys: 0.158 ± 0.152
1.735ProAsp: 1.735 ± 0.365
2.129ProGlu: 2.129 ± 0.5
0.868ProPhe: 0.868 ± 0.313
1.104ProGly: 1.104 ± 0.284
0.394ProHis: 0.394 ± 0.191
1.262ProIle: 1.262 ± 0.459
3.155ProLys: 3.155 ± 0.458
1.893ProLeu: 1.893 ± 0.446
0.552ProMet: 0.552 ± 0.212
1.104ProAsn: 1.104 ± 0.289
0.552ProPro: 0.552 ± 0.249
0.946ProGln: 0.946 ± 0.366
0.946ProArg: 0.946 ± 0.322
1.104ProSer: 1.104 ± 0.335
1.814ProThr: 1.814 ± 0.479
2.445ProVal: 2.445 ± 0.531
0.237ProTrp: 0.237 ± 0.133
0.71ProTyr: 0.71 ± 0.374
0.0ProXaa: 0.0 ± 0.0
Gln
3.391GlnAla: 3.391 ± 0.726
0.158GlnCys: 0.158 ± 0.139
1.656GlnAsp: 1.656 ± 0.486
3.864GlnGlu: 3.864 ± 0.559
1.893GlnPhe: 1.893 ± 0.284
2.208GlnGly: 2.208 ± 0.412
0.473GlnHis: 0.473 ± 0.162
2.366GlnIle: 2.366 ± 0.448
3.076GlnLys: 3.076 ± 0.513
3.233GlnLeu: 3.233 ± 0.455
0.946GlnMet: 0.946 ± 0.272
1.972GlnAsn: 1.972 ± 0.369
1.262GlnPro: 1.262 ± 0.41
0.789GlnGln: 0.789 ± 0.228
2.445GlnArg: 2.445 ± 0.547
2.681GlnSer: 2.681 ± 0.399
3.233GlnThr: 3.233 ± 0.723
2.997GlnVal: 2.997 ± 0.65
0.394GlnTrp: 0.394 ± 0.233
1.025GlnTyr: 1.025 ± 0.359
0.0GlnXaa: 0.0 ± 0.0
Arg
3.864ArgAla: 3.864 ± 0.817
0.315ArgCys: 0.315 ± 0.145
2.76ArgAsp: 2.76 ± 0.569
3.155ArgGlu: 3.155 ± 0.784
2.208ArgPhe: 2.208 ± 0.502
2.287ArgGly: 2.287 ± 0.523
0.946ArgHis: 0.946 ± 0.328
2.524ArgIle: 2.524 ± 0.618
3.707ArgLys: 3.707 ± 0.873
4.968ArgLeu: 4.968 ± 0.761
1.42ArgMet: 1.42 ± 0.332
3.785ArgAsn: 3.785 ± 0.647
1.42ArgPro: 1.42 ± 0.335
2.287ArgGln: 2.287 ± 0.731
2.76ArgArg: 2.76 ± 0.694
2.287ArgSer: 2.287 ± 0.424
3.233ArgThr: 3.233 ± 0.747
2.05ArgVal: 2.05 ± 0.437
0.552ArgTrp: 0.552 ± 0.216
2.524ArgTyr: 2.524 ± 0.766
0.0ArgXaa: 0.0 ± 0.0
Ser
4.101SerAla: 4.101 ± 0.78
0.315SerCys: 0.315 ± 0.151
3.785SerAsp: 3.785 ± 0.705
5.363SerGlu: 5.363 ± 0.618
2.603SerPhe: 2.603 ± 0.444
5.284SerGly: 5.284 ± 0.943
0.868SerHis: 0.868 ± 0.267
3.47SerIle: 3.47 ± 0.448
5.521SerLys: 5.521 ± 0.933
5.442SerLeu: 5.442 ± 1.013
1.735SerMet: 1.735 ± 0.445
3.233SerAsn: 3.233 ± 0.591
1.735SerPro: 1.735 ± 0.402
2.524SerGln: 2.524 ± 0.567
2.839SerArg: 2.839 ± 0.742
4.338SerSer: 4.338 ± 1.246
4.495SerThr: 4.495 ± 0.855
4.18SerVal: 4.18 ± 1.041
0.946SerTrp: 0.946 ± 0.326
2.287SerTyr: 2.287 ± 0.548
0.0SerXaa: 0.0 ± 0.0
Thr
5.757ThrAla: 5.757 ± 1.244
0.079ThrCys: 0.079 ± 0.095
4.495ThrAsp: 4.495 ± 0.682
3.155ThrGlu: 3.155 ± 0.519
3.233ThrPhe: 3.233 ± 0.765
3.549ThrGly: 3.549 ± 0.652
0.394ThrHis: 0.394 ± 0.144
4.653ThrIle: 4.653 ± 0.636
5.126ThrLys: 5.126 ± 0.721
4.495ThrLeu: 4.495 ± 0.622
0.868ThrMet: 0.868 ± 0.251
3.47ThrAsn: 3.47 ± 0.579
1.42ThrPro: 1.42 ± 0.402
2.129ThrGln: 2.129 ± 0.874
2.366ThrArg: 2.366 ± 0.381
3.943ThrSer: 3.943 ± 0.669
4.259ThrThr: 4.259 ± 0.657
3.549ThrVal: 3.549 ± 0.512
0.552ThrTrp: 0.552 ± 0.216
2.208ThrTyr: 2.208 ± 0.448
0.0ThrXaa: 0.0 ± 0.0
Val
3.864ValAla: 3.864 ± 0.552
0.315ValCys: 0.315 ± 0.174
3.233ValAsp: 3.233 ± 0.499
6.467ValGlu: 6.467 ± 0.968
1.814ValPhe: 1.814 ± 0.443
4.89ValGly: 4.89 ± 0.649
0.946ValHis: 0.946 ± 0.285
2.997ValIle: 2.997 ± 0.452
5.284ValLys: 5.284 ± 0.684
4.338ValLeu: 4.338 ± 0.694
1.341ValMet: 1.341 ± 0.391
2.918ValAsn: 2.918 ± 0.374
1.104ValPro: 1.104 ± 0.353
2.05ValGln: 2.05 ± 0.35
3.233ValArg: 3.233 ± 0.592
3.943ValSer: 3.943 ± 0.542
4.416ValThr: 4.416 ± 0.668
4.101ValVal: 4.101 ± 0.784
0.946ValTrp: 0.946 ± 0.317
2.287ValTyr: 2.287 ± 0.558
0.0ValXaa: 0.0 ± 0.0
Trp
0.71TrpAla: 0.71 ± 0.202
0.158TrpCys: 0.158 ± 0.133
0.473TrpAsp: 0.473 ± 0.259
0.789TrpGlu: 0.789 ± 0.235
0.631TrpPhe: 0.631 ± 0.374
0.789TrpGly: 0.789 ± 0.196
0.237TrpHis: 0.237 ± 0.214
1.262TrpIle: 1.262 ± 0.292
0.789TrpLys: 0.789 ± 0.257
0.71TrpLeu: 0.71 ± 0.222
0.473TrpMet: 0.473 ± 0.226
0.868TrpAsn: 0.868 ± 0.337
0.079TrpPro: 0.079 ± 0.088
1.025TrpGln: 1.025 ± 0.247
0.394TrpArg: 0.394 ± 0.232
0.71TrpSer: 0.71 ± 0.275
0.868TrpThr: 0.868 ± 0.289
0.71TrpVal: 0.71 ± 0.24
0.237TrpTrp: 0.237 ± 0.12
0.789TrpTyr: 0.789 ± 0.526
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.445TyrAla: 2.445 ± 0.454
0.315TyrCys: 0.315 ± 0.147
1.814TyrAsp: 1.814 ± 0.385
2.918TyrGlu: 2.918 ± 0.659
1.656TyrPhe: 1.656 ± 0.414
2.05TyrGly: 2.05 ± 0.636
0.71TyrHis: 0.71 ± 0.212
2.681TyrIle: 2.681 ± 0.447
2.208TyrLys: 2.208 ± 0.323
3.312TyrLeu: 3.312 ± 0.58
0.789TyrMet: 0.789 ± 0.33
2.129TyrAsn: 2.129 ± 0.377
1.183TyrPro: 1.183 ± 0.333
2.129TyrGln: 2.129 ± 0.381
1.814TyrArg: 1.814 ± 0.473
1.814TyrSer: 1.814 ± 0.496
1.972TyrThr: 1.972 ± 0.292
1.735TyrVal: 1.735 ± 0.355
0.552TyrTrp: 0.552 ± 0.139
1.42TyrTyr: 1.42 ± 0.712
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (12681 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski