Odonata-associated circular virus 5
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UI30|A0A0B4UI30_9VIRU Putative capsid protein OS=Odonata-associated circular virus 5 OX=1592125 PE=4 SV=1
MM1 pKa = 6.58 TTQFAQASYY10 pKa = 11.25 QEE12 pKa = 4.91 IIDD15 pKa = 4.59 LNTVTDD21 pKa = 3.88 KK22 pKa = 11.47 VSVLGIHH29 pKa = 6.06 TPVTDD34 pKa = 3.52 TPYY37 pKa = 10.8 TFLKK41 pKa = 10.56 PFFDD45 pKa = 4.44 AFQKK49 pKa = 10.26 YY50 pKa = 9.78 HH51 pKa = 6.82 YY52 pKa = 9.01 DD53 pKa = 3.61 GCSLTMVPAARR64 pKa = 11.84 LPADD68 pKa = 4.18 PSQVSYY74 pKa = 11.24 EE75 pKa = 4.24 SGEE78 pKa = 3.99 QPIDD82 pKa = 3.51 PRR84 pKa = 11.84 DD85 pKa = 3.77 LLNPILWHH93 pKa = 5.94 GAHH96 pKa = 6.59 GEE98 pKa = 4.17 SLGAVLNQFYY108 pKa = 10.25 DD109 pKa = 3.64 ASGSTSDD116 pKa = 3.23 IGRR119 pKa = 11.84 EE120 pKa = 3.86 FFDD123 pKa = 3.23 SGEE126 pKa = 4.05 VSRR129 pKa = 11.84 ASLTQVGNDD138 pKa = 3.32 ALYY141 pKa = 10.24 EE142 pKa = 4.1 SLYY145 pKa = 10.78 YY146 pKa = 10.17 RR147 pKa = 11.84 ALTDD151 pKa = 3.47 NSWRR155 pKa = 11.84 KK156 pKa = 8.79 AHH158 pKa = 6.44 PQTGLRR164 pKa = 11.84 VKK166 pKa = 10.28 GLHH169 pKa = 6.07 PLVYY173 pKa = 10.45 SVGTDD178 pKa = 3.12 AQYY181 pKa = 11.78 LNNPTGTTTPQVPRR195 pKa = 11.84 SANSPLPPSDD205 pKa = 4.26 AGPMTSVGAAGTMGKK220 pKa = 8.35 GTSSPYY226 pKa = 10.79 GLQSLNPQGLQDD238 pKa = 3.78 TSDD241 pKa = 3.96 SGDD244 pKa = 3.28 LTYY247 pKa = 10.92 VRR249 pKa = 11.84 SLPTPRR255 pKa = 11.84 TSQFFTEE262 pKa = 4.4 RR263 pKa = 11.84 LHH265 pKa = 6.68 GLGWLDD271 pKa = 3.12 TRR273 pKa = 11.84 VRR275 pKa = 11.84 ISSTSGFQAEE285 pKa = 4.45 VSGNQTLDD293 pKa = 2.9 RR294 pKa = 11.84 MAVEE298 pKa = 4.04 NLYY301 pKa = 10.59 EE302 pKa = 4.01 QVEE305 pKa = 4.39 DD306 pKa = 3.78 SQTINYY312 pKa = 9.09 LPRR315 pKa = 11.84 LFMGVCLLPPSYY327 pKa = 10.12 KK328 pKa = 10.29 AKK330 pKa = 9.93 QYY332 pKa = 9.55 YY333 pKa = 9.81 RR334 pKa = 11.84 LVINHH339 pKa = 6.76 RR340 pKa = 11.84 FSFRR344 pKa = 11.84 KK345 pKa = 9.56 FRR347 pKa = 11.84 GLSMDD352 pKa = 3.11 GGLRR356 pKa = 11.84 GRR358 pKa = 11.84 YY359 pKa = 9.04 GPARR363 pKa = 11.84 APSYY367 pKa = 10.67 RR368 pKa = 11.84 NLMM371 pKa = 3.92
Molecular weight: 40.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.08
IPC2_protein 6.046
IPC_protein 6.173
Toseland 6.634
ProMoST 6.517
Dawson 6.504
Bjellqvist 6.453
Wikipedia 6.532
Rodwell 6.491
Grimsley 6.81
Solomon 6.504
Lehninger 6.504
Nozaki 6.751
DTASelect 6.956
Thurlkill 6.985
EMBOSS 6.956
Sillero 6.883
Patrickios 3.859
IPC_peptide 6.517
IPC2_peptide 6.795
IPC2.peptide.svr19 6.786
Protein with the highest isoelectric point:
>tr|A0A0B4UI30|A0A0B4UI30_9VIRU Putative capsid protein OS=Odonata-associated circular virus 5 OX=1592125 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.57 WSIYY6 pKa = 9.67 IICGPIRR13 pKa = 11.84 IVIRR17 pKa = 11.84 RR18 pKa = 11.84 YY19 pKa = 10.46 RR20 pKa = 11.84 SMTEE24 pKa = 3.96 PKK26 pKa = 9.25 WWDD29 pKa = 3.24 CTLSRR34 pKa = 11.84 EE35 pKa = 4.24 EE36 pKa = 4.31 TFGNGNPNPYY46 pKa = 8.35 YY47 pKa = 9.39 TWHH50 pKa = 6.74 APDD53 pKa = 3.31 VGKK56 pKa = 10.26 QLMEE60 pKa = 4.95 HH61 pKa = 5.79 GAEE64 pKa = 3.68 RR65 pKa = 11.84 CVVGEE70 pKa = 3.99 EE71 pKa = 4.11 VGEE74 pKa = 4.35 DD75 pKa = 4.11 GYY77 pKa = 10.51 QHH79 pKa = 5.77 FQIRR83 pKa = 11.84 VVFKK87 pKa = 10.79 KK88 pKa = 8.84 PTSFEE93 pKa = 3.84 KK94 pKa = 10.23 ATAILPGSHH103 pKa = 6.21 WSEE106 pKa = 3.74 TSQFGRR112 pKa = 11.84 NFDD115 pKa = 3.81 YY116 pKa = 11.28 VEE118 pKa = 4.46 KK119 pKa = 10.5 EE120 pKa = 3.85 GHH122 pKa = 5.91 FWRR125 pKa = 11.84 SWEE128 pKa = 4.07 TALGRR133 pKa = 11.84 FMQLDD138 pKa = 4.03 MYY140 pKa = 9.9 PWQNEE145 pKa = 3.09 IMTRR149 pKa = 11.84 LEE151 pKa = 3.88 KK152 pKa = 11.04 QNDD155 pKa = 3.73 RR156 pKa = 11.84 KK157 pKa = 11.12 VMVIVDD163 pKa = 4.02 RR164 pKa = 11.84 YY165 pKa = 9.53 GNSGKK170 pKa = 8.01 TAIAMRR176 pKa = 11.84 LTAEE180 pKa = 4.48 HH181 pKa = 6.77 KK182 pKa = 10.43 GAYY185 pKa = 9.7 CPEE188 pKa = 4.92 LEE190 pKa = 4.71 DD191 pKa = 5.04 AKK193 pKa = 11.38 DD194 pKa = 3.65 YY195 pKa = 8.56 MAWALAHH202 pKa = 6.89 KK203 pKa = 10.34 NAGCFCLDD211 pKa = 3.14 IPRR214 pKa = 11.84 AGDD217 pKa = 3.34 VRR219 pKa = 11.84 KK220 pKa = 9.03 DD221 pKa = 3.24 TAIWKK226 pKa = 9.82 AVEE229 pKa = 3.91 QMKK232 pKa = 10.71 NGYY235 pKa = 9.2 LWDD238 pKa = 4.31 KK239 pKa = 9.19 RR240 pKa = 11.84 HH241 pKa = 5.79 HH242 pKa = 5.03 WQEE245 pKa = 3.73 AFIMPSKK252 pKa = 10.94 GLVLTNDD259 pKa = 3.35 EE260 pKa = 4.43 PDD262 pKa = 3.49 RR263 pKa = 11.84 NLLSRR268 pKa = 11.84 DD269 pKa = 2.98 RR270 pKa = 11.84 WDD272 pKa = 4.13 IGHH275 pKa = 7.69 LDD277 pKa = 3.58 YY278 pKa = 11.53 GISNWANTIKK288 pKa = 10.11 WDD290 pKa = 3.62 SS291 pKa = 3.32
Molecular weight: 34.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.36
IPC2_protein 6.326
IPC_protein 6.465
Toseland 6.664
ProMoST 6.81
Dawson 6.868
Bjellqvist 6.795
Wikipedia 6.868
Rodwell 6.868
Grimsley 6.781
Solomon 6.883
Lehninger 6.883
Nozaki 7.117
DTASelect 7.263
Thurlkill 7.293
EMBOSS 7.293
Sillero 7.263
Patrickios 4.266
IPC_peptide 6.898
IPC2_peptide 6.971
IPC2.peptide.svr19 6.89
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
662
291
371
331.0
37.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.344 ± 0.119
1.208 ± 0.547
6.193 ± 0.215
4.985 ± 1.432
3.776 ± 0.218
8.006 ± 0.507
2.719 ± 0.46
3.927 ± 1.228
4.23 ± 1.475
8.006 ± 1.609
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.87 ± 0.584
4.079 ± 0.249
5.891 ± 1.134
4.381 ± 0.826
6.495 ± 0.242
7.402 ± 1.883
6.647 ± 1.178
5.287 ± 0.305
2.719 ± 1.563
4.834 ± 0.456
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here