cyanobacterium G8-9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; unclassified Cyanobacteria

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 461 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4CZV6|A0A2T4CZV6_9CYAN Ubiquinol-cytochrome c reductase iron-sulfur subunit (Fragment) OS=cyanobacterium G8-9 OX=2137193 GN=C9925_01935 PE=4 SV=1
NN1 pKa = 7.62LYY3 pKa = 9.84YY4 pKa = 10.57HH5 pKa = 6.53QGDD8 pKa = 3.42AVEE11 pKa = 3.85YY12 pKa = 9.58GIRR15 pKa = 11.84FGAQSEE21 pKa = 4.34EE22 pKa = 3.77WRR24 pKa = 11.84ATFAFDD30 pKa = 4.32YY31 pKa = 10.31FDD33 pKa = 5.49SSSDD37 pKa = 3.54DD38 pKa = 3.6QNVEE42 pKa = 3.68KK43 pKa = 10.47AYY45 pKa = 11.21LLVDD49 pKa = 3.42YY50 pKa = 10.11FLFSTDD56 pKa = 2.74SEE58 pKa = 4.63LNIRR62 pKa = 11.84PFVGANVGLINYY74 pKa = 7.65EE75 pKa = 3.95STLIDD80 pKa = 3.26ATDD83 pKa = 3.82FVYY86 pKa = 10.53GGQAGVVVGLDD97 pKa = 3.48DD98 pKa = 6.81SIDD101 pKa = 3.87LDD103 pKa = 3.69LSYY106 pKa = 10.62RR107 pKa = 11.84YY108 pKa = 9.77SLSGSEE114 pKa = 4.51RR115 pKa = 11.84VNDD118 pKa = 3.44MGSIIFGLNYY128 pKa = 10.49LYY130 pKa = 11.15

Molecular weight: 14.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4D2I5|A0A2T4D2I5_9CYAN 30S ribosomal protein S3 OS=cyanobacterium G8-9 OX=2137193 GN=rpsC PE=3 SV=1
MM1 pKa = 7.59AKK3 pKa = 10.28KK4 pKa = 10.89SMIAKK9 pKa = 9.87QKK11 pKa = 8.37RR12 pKa = 11.84TPKK15 pKa = 10.15FSSQAYY21 pKa = 5.28TRR23 pKa = 11.84CNICGRR29 pKa = 11.84PHH31 pKa = 5.89SVYY34 pKa = 10.48RR35 pKa = 11.84DD36 pKa = 3.6FGLCRR41 pKa = 11.84VCLRR45 pKa = 11.84KK46 pKa = 8.66MANEE50 pKa = 3.73GLIPGMRR57 pKa = 11.84KK58 pKa = 9.7ASWW61 pKa = 2.91

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

461

0

461

101100

36

1478

219.3

24.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.967 ± 0.125

0.923 ± 0.049

5.455 ± 0.095

7.115 ± 0.141

4.673 ± 0.108

6.42 ± 0.131

1.947 ± 0.054

7.86 ± 0.121

8.276 ± 0.152

9.521 ± 0.146

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.952 ± 0.062

4.5 ± 0.091

3.419 ± 0.082

2.939 ± 0.061

3.707 ± 0.081

6.247 ± 0.106

5.418 ± 0.104

6.686 ± 0.115

1.008 ± 0.058

3.966 ± 0.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski