Streptococcus satellite phage Javan50

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZPA6|A0A4D5ZPA6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan50 OX=2558725 GN=JavanS50_0021 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84TFSDD6 pKa = 3.67TPKK9 pKa = 10.21TFTFHH14 pKa = 5.7YY15 pKa = 8.55TFKK18 pKa = 11.1DD19 pKa = 3.09FDD21 pKa = 4.09TAQVACHH28 pKa = 6.88AILGYY33 pKa = 7.75MTGTYY38 pKa = 8.49HH39 pKa = 7.19APVIGVTIKK48 pKa = 11.08GKK50 pKa = 9.59GQLVLEE56 pKa = 4.2YY57 pKa = 11.17VEE59 pKa = 5.17DD60 pKa = 3.83KK61 pKa = 11.02KK62 pKa = 11.15LSKK65 pKa = 10.24VFKK68 pKa = 10.17RR69 pKa = 11.84ICDD72 pKa = 3.69SFKK75 pKa = 10.93DD76 pKa = 4.21YY77 pKa = 11.52YY78 pKa = 10.51NQPEE82 pKa = 4.31DD83 pKa = 3.62MTDD86 pKa = 3.31EE87 pKa = 4.68EE88 pKa = 5.58LDD90 pKa = 4.63DD91 pKa = 3.7MAQEE95 pKa = 4.07NEE97 pKa = 4.3LIKK100 pKa = 10.55EE101 pKa = 4.12VEE103 pKa = 4.12EE104 pKa = 5.42LDD106 pKa = 3.9DD107 pKa = 3.76QRR109 pKa = 11.84VVPLFEE115 pKa = 4.1ITQEE119 pKa = 4.02EE120 pKa = 4.66ANEE123 pKa = 4.0QDD125 pKa = 3.13TLMAFISDD133 pKa = 3.57HH134 pKa = 6.56DD135 pKa = 3.74QLAEE139 pKa = 4.12HH140 pKa = 7.09LSMNYY145 pKa = 9.71QEE147 pKa = 4.96MSQDD151 pKa = 3.43DD152 pKa = 4.03LGAILEE158 pKa = 4.7TISQAFNHH166 pKa = 6.79LYY168 pKa = 11.22DD169 pKa = 3.83MVVEE173 pKa = 4.32GQLVVKK179 pKa = 10.63

Molecular weight: 20.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZP94|A0A4D5ZP94_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan50 OX=2558725 GN=JavanS50_0009 PE=4 SV=1
MM1 pKa = 7.38IMKK4 pKa = 8.9ITEE7 pKa = 4.08VKK9 pKa = 10.56KK10 pKa = 10.72KK11 pKa = 10.15DD12 pKa = 3.14GTVIYY17 pKa = 9.92RR18 pKa = 11.84ASIYY22 pKa = 10.71LGTDD26 pKa = 2.36KK27 pKa = 10.8VTGKK31 pKa = 10.64KK32 pKa = 7.91VTTKK36 pKa = 9.18ITGRR40 pKa = 11.84TKK42 pKa = 10.6KK43 pKa = 9.66EE44 pKa = 3.58VRR46 pKa = 11.84EE47 pKa = 4.02KK48 pKa = 10.86AKK50 pKa = 10.64QEE52 pKa = 3.62AVEE55 pKa = 4.71FIKK58 pKa = 11.15NGSTRR63 pKa = 11.84FKK65 pKa = 9.21ATSVTSYY72 pKa = 11.34QEE74 pKa = 4.91LATLWWDD81 pKa = 3.74SYY83 pKa = 11.1KK84 pKa = 9.85HH85 pKa = 4.21TVKK88 pKa = 10.92YY89 pKa = 8.4NTQLATEE96 pKa = 4.37KK97 pKa = 10.61LLTVHH102 pKa = 6.77VIPIFGAYY110 pKa = 9.53KK111 pKa = 10.15LDD113 pKa = 3.67KK114 pKa = 10.12LTTPLIQSIINKK126 pKa = 9.54LADD129 pKa = 3.45KK130 pKa = 9.75TNKK133 pKa = 10.06GEE135 pKa = 4.09RR136 pKa = 11.84KK137 pKa = 9.79AYY139 pKa = 9.6LHH141 pKa = 6.59YY142 pKa = 11.09DD143 pKa = 4.1RR144 pKa = 11.84IHH146 pKa = 6.67ALNKK150 pKa = 10.16RR151 pKa = 11.84ILQYY155 pKa = 10.96GVIMQAIPFNPARR168 pKa = 11.84EE169 pKa = 4.24VILPRR174 pKa = 11.84NTKK177 pKa = 9.3KK178 pKa = 10.99ANTKK182 pKa = 9.27RR183 pKa = 11.84VKK185 pKa = 10.5HH186 pKa = 5.63FEE188 pKa = 3.77NDD190 pKa = 3.02EE191 pKa = 3.81LRR193 pKa = 11.84TFFNYY198 pKa = 10.41LNNLDD203 pKa = 3.7KK204 pKa = 11.1SKK206 pKa = 10.86YY207 pKa = 8.12RR208 pKa = 11.84YY209 pKa = 9.24FYY211 pKa = 10.89EE212 pKa = 3.82VTLYY216 pKa = 11.0KK217 pKa = 10.48FLLATGCRR225 pKa = 11.84INEE228 pKa = 3.8ALALNWSDD236 pKa = 3.65IDD238 pKa = 3.87LDD240 pKa = 3.8NAVVHH245 pKa = 5.12VTKK248 pKa = 10.15TLNYY252 pKa = 8.35KK253 pKa = 9.4QEE255 pKa = 4.1INSPKK260 pKa = 10.23SKK262 pKa = 10.48SSYY265 pKa = 10.17RR266 pKa = 11.84DD267 pKa = 2.69IDD269 pKa = 3.27IDD271 pKa = 3.87SRR273 pKa = 11.84TVTMLKK279 pKa = 9.73QYY281 pKa = 10.25RR282 pKa = 11.84RR283 pKa = 11.84RR284 pKa = 11.84QIQEE288 pKa = 2.96AWKK291 pKa = 9.76LGRR294 pKa = 11.84SEE296 pKa = 4.29TVVFSDD302 pKa = 6.41FIHH305 pKa = 7.11KK306 pKa = 10.24YY307 pKa = 8.57PNNRR311 pKa = 11.84TLQTRR316 pKa = 11.84LRR318 pKa = 11.84THH320 pKa = 6.83FKK322 pKa = 10.02RR323 pKa = 11.84ANVSNIGFHH332 pKa = 6.27GFRR335 pKa = 11.84HH336 pKa = 4.87THH338 pKa = 6.65ASLLLNTGIPYY349 pKa = 10.09KK350 pKa = 10.14EE351 pKa = 3.64LQYY354 pKa = 11.41RR355 pKa = 11.84LGHH358 pKa = 5.67STLSMTMDD366 pKa = 3.58IYY368 pKa = 11.68SHH370 pKa = 6.98LSKK373 pKa = 11.0EE374 pKa = 4.25NAKK377 pKa = 10.34KK378 pKa = 10.33AVSFFEE384 pKa = 4.08TAINSII390 pKa = 4.07

Molecular weight: 45.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

3645

46

541

158.5

18.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.35 ± 0.227

0.631 ± 0.238

5.816 ± 0.449

7.435 ± 0.468

4.39 ± 0.21

4.856 ± 0.478

1.619 ± 0.372

7.627 ± 0.45

9.328 ± 0.526

9.465 ± 0.437

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.223

5.652 ± 0.363

3.347 ± 0.384

4.664 ± 0.378

4.719 ± 0.415

5.514 ± 0.426

6.365 ± 0.509

5.048 ± 0.388

0.933 ± 0.134

5.048 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski