Streptomyces scabichelini

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9567 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G4VF27|A0A6G4VF27_9ACTN Amino acid ABC transporter permease OS=Streptomyces scabichelini OX=2711217 GN=G5C60_35080 PE=3 SV=1
MM1 pKa = 7.15AQKK4 pKa = 10.53SYY6 pKa = 9.67TVTGMSCEE14 pKa = 3.88HH15 pKa = 6.83CAASITEE22 pKa = 4.26EE23 pKa = 4.15VSDD26 pKa = 4.08VPGVTDD32 pKa = 3.4VVVDD36 pKa = 4.18LASNVVTVHH45 pKa = 5.37ATEE48 pKa = 5.16LDD50 pKa = 3.78DD51 pKa = 4.63DD52 pKa = 4.09QVRR55 pKa = 11.84AAIVEE60 pKa = 3.97AGYY63 pKa = 10.87GVDD66 pKa = 4.06EE67 pKa = 4.58PASVV71 pKa = 3.11

Molecular weight: 7.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G4V5B5|A0A6G4V5B5_9ACTN MFS transporter OS=Streptomyces scabichelini OX=2711217 GN=G5C60_15855 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.48GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9567

0

9567

3151775

20

7787

329.4

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.143 ± 0.034

0.759 ± 0.006

6.058 ± 0.02

5.819 ± 0.023

2.782 ± 0.014

9.303 ± 0.026

2.273 ± 0.013

3.351 ± 0.014

2.275 ± 0.024

10.32 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.802 ± 0.009

1.863 ± 0.014

5.952 ± 0.02

2.852 ± 0.015

7.876 ± 0.028

5.281 ± 0.021

6.162 ± 0.019

8.441 ± 0.022

1.534 ± 0.011

2.153 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski