Amino acid dipepetide frequency for Roseburia sp. CAG:197

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.509AlaAla: 6.509 ± 0.114	1.146AlaCys: 1.146 ± 0.044	4.813AlaAsp: 4.813 ± 0.08	5.048AlaGlu: 5.048 ± 0.096	3.069AlaPhe: 3.069 ± 0.067	5.607AlaGly: 5.607 ± 0.1	1.157AlaHis: 1.157 ± 0.041	5.518AlaIle: 5.518 ± 0.098	5.545AlaLys: 5.545 ± 0.108	6.461AlaLeu: 6.461 ± 0.101	2.448AlaMet: 2.448 ± 0.061	2.953AlaAsn: 2.953 ± 0.062	1.792AlaPro: 1.792 ± 0.052	2.656AlaGln: 2.656 ± 0.065	2.595AlaArg: 2.595 ± 0.058	4.087AlaSer: 4.087 ± 0.08	3.752AlaThr: 3.752 ± 0.079	5.688AlaVal: 5.688 ± 0.097	0.626AlaTrp: 0.626 ± 0.031	3.05AlaTyr: 3.05 ± 0.066	0.007AlaXaa: 0.007 ± 0.003
Cys	1.026CysAla: 1.026 ± 0.039	0.24CysCys: 0.24 ± 0.02	0.872CysAsp: 0.872 ± 0.038	0.914CysGlu: 0.914 ± 0.036	0.589CysPhe: 0.589 ± 0.03	1.405CysGly: 1.405 ± 0.051	0.289CysHis: 0.289 ± 0.021	1.155CysIle: 1.155 ± 0.045	0.902CysLys: 0.902 ± 0.034	1.122CysLeu: 1.122 ± 0.043	0.487CysMet: 0.487 ± 0.026	0.623CysAsn: 0.623 ± 0.025	0.542CysPro: 0.542 ± 0.033	0.426CysGln: 0.426 ± 0.024	0.631CysArg: 0.631 ± 0.029	0.86CysSer: 0.86 ± 0.038	0.752CysThr: 0.752 ± 0.031	1.107CysVal: 1.107 ± 0.044	0.124CysTrp: 0.124 ± 0.013	0.653CysTyr: 0.653 ± 0.033	0.0CysXaa: 0.0 ± 0.0
Asp	4.921AspAla: 4.921 ± 0.094	0.805AspCys: 0.805 ± 0.033	3.461AspAsp: 3.461 ± 0.081	4.879AspGlu: 4.879 ± 0.085	2.675AspPhe: 2.675 ± 0.063	4.432AspGly: 4.432 ± 0.093	1.049AspHis: 1.049 ± 0.037	4.715AspIle: 4.715 ± 0.085	3.813AspLys: 3.813 ± 0.077	4.614AspLeu: 4.614 ± 0.084	1.937AspMet: 1.937 ± 0.056	2.618AspAsn: 2.618 ± 0.079	1.555AspPro: 1.555 ± 0.052	1.534AspGln: 1.534 ± 0.05	2.188AspArg: 2.188 ± 0.059	3.187AspSer: 3.187 ± 0.075	3.353AspThr: 3.353 ± 0.073	4.153AspVal: 4.153 ± 0.083	0.605AspTrp: 0.605 ± 0.034	2.916AspTyr: 2.916 ± 0.071	0.003AspXaa: 0.003 ± 0.002
Glu	5.194GluAla: 5.194 ± 0.092	0.837GluCys: 0.837 ± 0.041	4.428GluAsp: 4.428 ± 0.099	6.716GluGlu: 6.716 ± 0.123	2.439GluPhe: 2.439 ± 0.063	3.923GluGly: 3.923 ± 0.066	1.484GluHis: 1.484 ± 0.052	5.555GluIle: 5.555 ± 0.098	7.003GluLys: 7.003 ± 0.098	6.36GluLeu: 6.36 ± 0.102	2.289GluMet: 2.289 ± 0.05	4.329GluAsn: 4.329 ± 0.082	1.754GluPro: 1.754 ± 0.051	3.186GluGln: 3.186 ± 0.07	3.016GluArg: 3.016 ± 0.075	3.48GluSer: 3.48 ± 0.071	3.787GluThr: 3.787 ± 0.084	4.527GluVal: 4.527 ± 0.085	0.607GluTrp: 0.607 ± 0.027	2.896GluTyr: 2.896 ± 0.067	0.0GluXaa: 0.0 ± 0.0
Phe	2.997PheAla: 2.997 ± 0.066	0.778PheCys: 0.778 ± 0.037	2.578PheAsp: 2.578 ± 0.067	2.555PheGlu: 2.555 ± 0.069	1.75PhePhe: 1.75 ± 0.052	2.883PheGly: 2.883 ± 0.062	0.87PheHis: 0.87 ± 0.034	2.723PheIle: 2.723 ± 0.076	1.9PheLys: 1.9 ± 0.053	3.667PheLeu: 3.667 ± 0.083	1.199PheMet: 1.199 ± 0.041	1.467PheAsn: 1.467 ± 0.044	1.243PhePro: 1.243 ± 0.041	1.247PheGln: 1.247 ± 0.045	1.505PheArg: 1.505 ± 0.054	2.833PheSer: 2.833 ± 0.063	2.266PheThr: 2.266 ± 0.064	3.081PheVal: 3.081 ± 0.064	0.407PheTrp: 0.407 ± 0.026	1.753PheTyr: 1.753 ± 0.053	0.0PheXaa: 0.0 ± 0.0
Gly	4.711GlyAla: 4.711 ± 0.097	1.184GlyCys: 1.184 ± 0.041	3.477GlyAsp: 3.477 ± 0.077	4.395GlyGlu: 4.395 ± 0.074	2.88GlyPhe: 2.88 ± 0.064	4.255GlyGly: 4.255 ± 0.094	1.205GlyHis: 1.205 ± 0.046	5.926GlyIle: 5.926 ± 0.1	5.649GlyLys: 5.649 ± 0.101	5.268GlyLeu: 5.268 ± 0.098	2.488GlyMet: 2.488 ± 0.063	3.415GlyAsn: 3.415 ± 0.085	1.155GlyPro: 1.155 ± 0.043	2.071GlyGln: 2.071 ± 0.054	2.562GlyArg: 2.562 ± 0.064	3.732GlySer: 3.732 ± 0.074	4.189GlyThr: 4.189 ± 0.08	4.74GlyVal: 4.74 ± 0.092	0.589GlyTrp: 0.589 ± 0.03	3.195GlyTyr: 3.195 ± 0.075	0.001GlyXaa: 0.001 ± 0.001
His	1.262HisAla: 1.262 ± 0.049	0.305HisCys: 0.305 ± 0.019	0.902HisAsp: 0.902 ± 0.041	1.111HisGlu: 1.111 ± 0.041	0.88HisPhe: 0.88 ± 0.039	1.236HisGly: 1.236 ± 0.046	0.425HisHis: 0.425 ± 0.033	1.401HisIle: 1.401 ± 0.045	1.13HisLys: 1.13 ± 0.041	1.525HisLeu: 1.525 ± 0.047	0.627HisMet: 0.627 ± 0.03	0.767HisAsn: 0.767 ± 0.036	0.79HisPro: 0.79 ± 0.036	0.569HisGln: 0.569 ± 0.031	0.773HisArg: 0.773 ± 0.036	0.907HisSer: 0.907 ± 0.035	0.994HisThr: 0.994 ± 0.037	1.236HisVal: 1.236 ± 0.047	0.159HisTrp: 0.159 ± 0.014	0.813HisTyr: 0.813 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.985IleAla: 5.985 ± 0.092	1.393IleCys: 1.393 ± 0.047	4.407IleAsp: 4.407 ± 0.087	4.959IleGlu: 4.959 ± 0.09	2.734IlePhe: 2.734 ± 0.076	5.214IleGly: 5.214 ± 0.098	1.37IleHis: 1.37 ± 0.048	5.227IleIle: 5.227 ± 0.118	4.201IleLys: 4.201 ± 0.081	6.86IleLeu: 6.86 ± 0.106	2.048IleMet: 2.048 ± 0.057	3.062IleAsn: 3.062 ± 0.065	3.027IlePro: 3.027 ± 0.062	2.315IleGln: 2.315 ± 0.053	3.555IleArg: 3.555 ± 0.072	4.962IleSer: 4.962 ± 0.089	4.42IleThr: 4.42 ± 0.085	5.566IleVal: 5.566 ± 0.103	0.58IleTrp: 0.58 ± 0.027	2.945IleTyr: 2.945 ± 0.068	0.0IleXaa: 0.0 ± 0.0
Lys	5.291LysAla: 5.291 ± 0.092	0.771LysCys: 0.771 ± 0.035	4.527LysAsp: 4.527 ± 0.089	6.728LysGlu: 6.728 ± 0.111	2.15LysPhe: 2.15 ± 0.053	3.985LysGly: 3.985 ± 0.073	1.077LysHis: 1.077 ± 0.039	5.145LysIle: 5.145 ± 0.08	7.278LysLys: 7.278 ± 0.124	5.704LysLeu: 5.704 ± 0.087	2.397LysMet: 2.397 ± 0.058	4.347LysAsn: 4.347 ± 0.089	1.853LysPro: 1.853 ± 0.055	2.661LysGln: 2.661 ± 0.06	2.927LysArg: 2.927 ± 0.066	3.617LysSer: 3.617 ± 0.073	4.122LysThr: 4.122 ± 0.075	4.874LysVal: 4.874 ± 0.095	0.572LysTrp: 0.572 ± 0.029	3.1LysTyr: 3.1 ± 0.082	0.0LysXaa: 0.0 ± 0.0
Leu	6.646LeuAla: 6.646 ± 0.09	1.425LeuCys: 1.425 ± 0.043	5.026LeuAsp: 5.026 ± 0.092	5.823LeuGlu: 5.823 ± 0.106	3.772LeuPhe: 3.772 ± 0.091	5.552LeuGly: 5.552 ± 0.102	1.611LeuHis: 1.611 ± 0.053	5.788LeuIle: 5.788 ± 0.103	5.694LeuLys: 5.694 ± 0.09	7.955LeuLeu: 7.955 ± 0.138	2.671LeuMet: 2.671 ± 0.066	3.608LeuAsn: 3.608 ± 0.068	2.962LeuPro: 2.962 ± 0.065	3.046LeuGln: 3.046 ± 0.068	3.461LeuArg: 3.461 ± 0.078	5.885LeuSer: 5.885 ± 0.098	4.804LeuThr: 4.804 ± 0.079	5.709LeuVal: 5.709 ± 0.093	0.702LeuTrp: 0.702 ± 0.031	3.356LeuTyr: 3.356 ± 0.08	0.001LeuXaa: 0.001 ± 0.001
Met	2.265MetAla: 2.265 ± 0.057	0.392MetCys: 0.392 ± 0.023	2.176MetAsp: 2.176 ± 0.055	2.525MetGlu: 2.525 ± 0.063	1.053MetPhe: 1.053 ± 0.036	2.009MetGly: 2.009 ± 0.062	0.516MetHis: 0.516 ± 0.029	2.297MetIle: 2.297 ± 0.064	2.786MetLys: 2.786 ± 0.065	2.779MetLeu: 2.779 ± 0.065	0.98MetMet: 0.98 ± 0.04	1.679MetAsn: 1.679 ± 0.051	1.1MetPro: 1.1 ± 0.036	1.363MetGln: 1.363 ± 0.044	1.302MetArg: 1.302 ± 0.041	1.784MetSer: 1.784 ± 0.049	1.719MetThr: 1.719 ± 0.048	2.088MetVal: 2.088 ± 0.052	0.235MetTrp: 0.235 ± 0.017	1.11MetTyr: 1.11 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	3.492AsnAla: 3.492 ± 0.076	0.686AsnCys: 0.686 ± 0.036	2.448AsnAsp: 2.448 ± 0.059	3.177AsnGlu: 3.177 ± 0.079	1.685AsnPhe: 1.685 ± 0.054	3.567AsnGly: 3.567 ± 0.088	0.901AsnHis: 0.901 ± 0.036	3.63AsnIle: 3.63 ± 0.077	2.918AsnLys: 2.918 ± 0.069	3.91AsnLeu: 3.91 ± 0.067	1.563AsnMet: 1.563 ± 0.045	2.331AsnAsn: 2.331 ± 0.084	1.879AsnPro: 1.879 ± 0.05	1.728AsnGln: 1.728 ± 0.059	2.126AsnArg: 2.126 ± 0.055	2.342AsnSer: 2.342 ± 0.062	2.618AsnThr: 2.618 ± 0.067	3.334AsnVal: 3.334 ± 0.073	0.41AsnTrp: 0.41 ± 0.025	2.122AsnTyr: 2.122 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	2.103ProAla: 2.103 ± 0.056	0.406ProCys: 0.406 ± 0.023	2.036ProAsp: 2.036 ± 0.052	2.835ProGlu: 2.835 ± 0.062	1.344ProPhe: 1.344 ± 0.044	2.022ProGly: 2.022 ± 0.055	0.488ProHis: 0.488 ± 0.026	2.087ProIle: 2.087 ± 0.053	2.067ProLys: 2.067 ± 0.056	2.354ProLeu: 2.354 ± 0.061	0.866ProMet: 0.866 ± 0.036	1.223ProAsn: 1.223 ± 0.042	0.566ProPro: 0.566 ± 0.031	1.195ProGln: 1.195 ± 0.045	0.777ProArg: 0.777 ± 0.033	1.51ProSer: 1.51 ± 0.043	1.507ProThr: 1.507 ± 0.044	2.637ProVal: 2.637 ± 0.063	0.305ProTrp: 0.305 ± 0.02	1.462ProTyr: 1.462 ± 0.047	0.001ProXaa: 0.001 ± 0.001
Gln	2.426GlnAla: 2.426 ± 0.064	0.368GlnCys: 0.368 ± 0.024	1.633GlnAsp: 1.633 ± 0.048	2.865GlnGlu: 2.865 ± 0.071	1.236GlnPhe: 1.236 ± 0.039	1.858GlnGly: 1.858 ± 0.051	0.516GlnHis: 0.516 ± 0.024	2.848GlnIle: 2.848 ± 0.062	3.353GlnLys: 3.353 ± 0.078	2.984GlnLeu: 2.984 ± 0.067	1.549GlnMet: 1.549 ± 0.054	1.805GlnAsn: 1.805 ± 0.051	1.003GlnPro: 1.003 ± 0.045	1.6GlnGln: 1.6 ± 0.054	1.329GlnArg: 1.329 ± 0.039	1.768GlnSer: 1.768 ± 0.055	1.987GlnThr: 1.987 ± 0.053	2.252GlnVal: 2.252 ± 0.055	0.313GlnTrp: 0.313 ± 0.023	1.416GlnTyr: 1.416 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	2.439ArgAla: 2.439 ± 0.063	0.515ArgCys: 0.515 ± 0.027	2.049ArgAsp: 2.049 ± 0.055	3.369ArgGlu: 3.369 ± 0.081	1.654ArgPhe: 1.654 ± 0.049	2.163ArgGly: 2.163 ± 0.061	0.693ArgHis: 0.693 ± 0.033	3.313ArgIle: 3.313 ± 0.07	3.376ArgLys: 3.376 ± 0.067	3.41ArgLeu: 3.41 ± 0.08	1.572ArgMet: 1.572 ± 0.05	2.028ArgAsn: 2.028 ± 0.048	1.162ArgPro: 1.162 ± 0.041	1.653ArgGln: 1.653 ± 0.054	1.851ArgArg: 1.851 ± 0.053	1.909ArgSer: 1.909 ± 0.048	2.083ArgThr: 2.083 ± 0.055	2.544ArgVal: 2.544 ± 0.059	0.294ArgTrp: 0.294 ± 0.02	1.751ArgTyr: 1.751 ± 0.048	0.001ArgXaa: 0.001 ± 0.001
Ser	4.36SerAla: 4.36 ± 0.084	0.775SerCys: 0.775 ± 0.033	3.524SerAsp: 3.524 ± 0.075	3.557SerGlu: 3.557 ± 0.073	2.517SerPhe: 2.517 ± 0.053	4.73SerGly: 4.73 ± 0.087	1.008SerHis: 1.008 ± 0.038	4.131SerIle: 4.131 ± 0.085	3.736SerLys: 3.736 ± 0.088	4.668SerLeu: 4.668 ± 0.078	1.944SerMet: 1.944 ± 0.05	2.589SerAsn: 2.589 ± 0.07	1.412SerPro: 1.412 ± 0.044	1.909SerGln: 1.909 ± 0.055	2.223SerArg: 2.223 ± 0.052	3.705SerSer: 3.705 ± 0.094	2.892SerThr: 2.892 ± 0.064	4.381SerVal: 4.381 ± 0.08	0.473SerTrp: 0.473 ± 0.027	2.707SerTyr: 2.707 ± 0.064	0.0SerXaa: 0.0 ± 0.0
Thr	4.01ThrAla: 4.01 ± 0.083	0.693ThrCys: 0.693 ± 0.036	3.518ThrAsp: 3.518 ± 0.074	3.813ThrGlu: 3.813 ± 0.079	2.134ThrPhe: 2.134 ± 0.054	4.482ThrGly: 4.482 ± 0.072	0.897ThrHis: 0.897 ± 0.037	4.413ThrIle: 4.413 ± 0.081	3.662ThrLys: 3.662 ± 0.075	4.794ThrLeu: 4.794 ± 0.093	1.522ThrMet: 1.522 ± 0.045	2.373ThrAsn: 2.373 ± 0.062	1.909ThrPro: 1.909 ± 0.057	1.805ThrGln: 1.805 ± 0.058	1.878ThrArg: 1.878 ± 0.054	3.21ThrSer: 3.21 ± 0.069	3.283ThrThr: 3.283 ± 0.077	4.463ThrVal: 4.463 ± 0.092	0.422ThrTrp: 0.422 ± 0.025	2.548ThrTyr: 2.548 ± 0.066	0.003ThrXaa: 0.003 ± 0.002
Val	5.444ValAla: 5.444 ± 0.1	1.247ValCys: 1.247 ± 0.044	4.188ValAsp: 4.188 ± 0.085	4.701ValGlu: 4.701 ± 0.083	2.901ValPhe: 2.901 ± 0.067	4.359ValGly: 4.359 ± 0.079	1.108ValHis: 1.108 ± 0.038	5.351ValIle: 5.351 ± 0.097	4.723ValLys: 4.723 ± 0.096	6.54ValLeu: 6.54 ± 0.112	2.076ValMet: 2.076 ± 0.053	3.119ValAsn: 3.119 ± 0.07	2.451ValPro: 2.451 ± 0.056	2.184ValGln: 2.184 ± 0.051	2.792ValArg: 2.792 ± 0.062	4.602ValSer: 4.602 ± 0.08	4.518ValThr: 4.518 ± 0.089	5.185ValVal: 5.185 ± 0.112	0.584ValTrp: 0.584 ± 0.031	2.888ValTyr: 2.888 ± 0.068	0.001ValXaa: 0.001 ± 0.002
Trp	0.471TrpAla: 0.471 ± 0.024	0.12TrpCys: 0.12 ± 0.013	0.492TrpAsp: 0.492 ± 0.027	0.56TrpGlu: 0.56 ± 0.027	0.364TrpPhe: 0.364 ± 0.024	0.593TrpGly: 0.593 ± 0.028	0.183TrpHis: 0.183 ± 0.014	0.685TrpIle: 0.685 ± 0.03	0.817TrpLys: 0.817 ± 0.034	0.75TrpLeu: 0.75 ± 0.032	0.28TrpMet: 0.28 ± 0.019	0.535TrpAsn: 0.535 ± 0.031	0.2TrpPro: 0.2 ± 0.018	0.305TrpGln: 0.305 ± 0.02	0.314TrpArg: 0.314 ± 0.025	0.404TrpSer: 0.404 ± 0.024	0.39TrpThr: 0.39 ± 0.027	0.48TrpVal: 0.48 ± 0.029	0.097TrpTrp: 0.097 ± 0.011	0.383TrpTyr: 0.383 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.977TyrAla: 2.977 ± 0.067	0.632TyrCys: 0.632 ± 0.031	2.856TyrAsp: 2.856 ± 0.064	3.279TyrGlu: 3.279 ± 0.075	1.871TyrPhe: 1.871 ± 0.06	2.81TyrGly: 2.81 ± 0.066	0.963TyrHis: 0.963 ± 0.038	2.92TyrIle: 2.92 ± 0.073	2.577TyrLys: 2.577 ± 0.07	3.771TyrLeu: 3.771 ± 0.078	1.213TyrMet: 1.213 ± 0.048	1.998TyrAsn: 1.998 ± 0.061	1.436TyrPro: 1.436 ± 0.047	1.653TyrGln: 1.653 ± 0.046	2.021TyrArg: 2.021 ± 0.057	2.457TyrSer: 2.457 ± 0.072	2.393TyrThr: 2.393 ± 0.058	2.916TyrVal: 2.916 ± 0.072	0.32TyrTrp: 0.32 ± 0.021	2.152TyrTyr: 2.152 ± 0.066	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.002	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.003XaaGly: 0.003 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.003XaaArg: 0.003 ± 0.002	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.003XaaVal: 0.003 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.024XaaXaa: 0.024 ± 0.007

Statistics based on 2420 proteins (741697 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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