Babjeviella inositovora NRRL Y-12698
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6394 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E3QT99|A0A1E3QT99_9ASCO Uncharacterized protein OS=Babjeviella inositovora NRRL Y-12698 OX=984486 GN=BABINDRAFT_160353 PE=4 SV=1
MM1 pKa = 7.69 LSTSEE6 pKa = 4.92 AYY8 pKa = 9.66 QGYY11 pKa = 10.2 KK12 pKa = 10.11 LANQVKK18 pKa = 9.91 HH19 pKa = 6.22 KK20 pKa = 9.17 LTRR23 pKa = 11.84 TARR26 pKa = 11.84 NPNADD31 pKa = 3.46 LRR33 pKa = 11.84 VLVTQANLLDD43 pKa = 4.19 RR44 pKa = 11.84 LLDD47 pKa = 3.8 SLSSSVCDD55 pKa = 3.77 SSSDD59 pKa = 3.75 DD60 pKa = 3.88 SSDD63 pKa = 3.26 SDD65 pKa = 4.07 VEE67 pKa = 4.07 VDD69 pKa = 3.71 VEE71 pKa = 4.28 VDD73 pKa = 3.3 AGPSVHH79 pKa = 7.09 FEE81 pKa = 3.56 IPAQRR86 pKa = 11.84 EE87 pKa = 3.51 QSTTVTCYY95 pKa = 10.29 EE96 pKa = 3.83 VDD98 pKa = 3.26 SDD100 pKa = 5.04 GEE102 pKa = 4.09 SDD104 pKa = 4.03 YY105 pKa = 11.67 DD106 pKa = 3.85 SQSDD110 pKa = 3.92 YY111 pKa = 11.85 DD112 pKa = 3.92 DD113 pKa = 5.21 DD114 pKa = 4.84 EE115 pKa = 6.45 YY116 pKa = 11.91 YY117 pKa = 11.06 DD118 pKa = 4.44 DD119 pKa = 5.6 DD120 pKa = 4.62 EE121 pKa = 5.43 YY122 pKa = 12.01 YY123 pKa = 10.88 NDD125 pKa = 5.25 SDD127 pKa = 4.43 IEE129 pKa = 5.25 DD130 pKa = 4.08 DD131 pKa = 4.73 AGEE134 pKa = 4.42 DD135 pKa = 3.78 FNKK138 pKa = 10.34 GVASFNPYY146 pKa = 8.95 TFSHH150 pKa = 6.76 HH151 pKa = 6.49 ISRR154 pKa = 11.84 VLTPVVEE161 pKa = 4.47 EE162 pKa = 4.44 EE163 pKa = 4.52 EE164 pKa = 4.21 EE165 pKa = 4.2 QEE167 pKa = 3.9 PHH169 pKa = 5.52 LTYY172 pKa = 11.01 SSGTDD177 pKa = 3.37 SEE179 pKa = 4.75 SDD181 pKa = 3.17 SSGHH185 pKa = 5.93 HH186 pKa = 6.35 NEE188 pKa = 3.36 YY189 pKa = 10.2 TYY191 pKa = 11.58 AKK193 pKa = 10.23 DD194 pKa = 3.98 RR195 pKa = 11.84 STTDD199 pKa = 3.01 TTRR202 pKa = 11.84 SISANNNVEE211 pKa = 4.15 SLMGKK216 pKa = 7.54 QLHH219 pKa = 5.71 MQSSDD224 pKa = 3.03 HH225 pKa = 7.01 QMVLLNSLIIEE236 pKa = 5.34 GYY238 pKa = 9.99
Molecular weight: 26.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.961
IPC_protein 3.973
Toseland 3.757
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.872
Rodwell 3.795
Grimsley 3.668
Solomon 3.948
Lehninger 3.91
Nozaki 4.062
DTASelect 4.304
Thurlkill 3.808
EMBOSS 3.884
Sillero 4.088
Patrickios 1.341
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 3.963
Protein with the highest isoelectric point:
>tr|A0A1E3QP52|A0A1E3QP52_9ASCO Tubulin-folding cofactor C (Fragment) OS=Babjeviella inositovora NRRL Y-12698 OX=984486 GN=BABINDRAFT_26651 PE=3 SV=1
MM1 pKa = 7.46 ARR3 pKa = 11.84 RR4 pKa = 11.84 YY5 pKa = 9.48 SAWKK9 pKa = 10.31 RR10 pKa = 11.84 LTQVNRR16 pKa = 11.84 IVLLCIRR23 pKa = 11.84 VTRR26 pKa = 11.84 SLLSKK31 pKa = 10.49 ASHH34 pKa = 5.76 TASTQEE40 pKa = 4.07 PKK42 pKa = 10.97 GGLNSYY48 pKa = 10.78 LKK50 pKa = 10.45 GKK52 pKa = 8.19 SFRR55 pKa = 11.84 IFSCSSYY62 pKa = 10.28 WSTHH66 pKa = 3.54 LCIRR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 EE73 pKa = 4.25 DD74 pKa = 3.36 IRR76 pKa = 11.84 DD77 pKa = 3.04 IDD79 pKa = 4.08 FKK81 pKa = 11.08 VSQGRR86 pKa = 11.84 APCFFQNEE94 pKa = 4.5 VISAGPTLPQLCCSPRR110 pKa = 11.84 LVQQNKK116 pKa = 8.92
Molecular weight: 13.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.56
IPC_protein 10.101
Toseland 10.54
ProMoST 10.248
Dawson 10.657
Bjellqvist 10.365
Wikipedia 10.833
Rodwell 10.95
Grimsley 10.701
Solomon 10.73
Lehninger 10.716
Nozaki 10.584
DTASelect 10.335
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.599
Patrickios 10.701
IPC_peptide 10.745
IPC2_peptide 9.677
IPC2.peptide.svr19 8.498
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6393
1
6394
2766287
49
5023
432.6
48.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.86 ± 0.039
1.231 ± 0.012
5.469 ± 0.023
6.391 ± 0.029
4.347 ± 0.018
5.785 ± 0.028
2.279 ± 0.012
5.394 ± 0.021
5.835 ± 0.028
10.055 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.168 ± 0.011
4.249 ± 0.019
4.953 ± 0.027
3.798 ± 0.022
4.951 ± 0.024
8.03 ± 0.034
5.963 ± 0.018
6.848 ± 0.026
1.096 ± 0.01
3.298 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here