Pseudoalteromonas phage PH1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kafunavirus; unclassified Kafunavirus

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2AP81|A0A1B2AP81_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage PH1 OX=1874540 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 7.8EE3 pKa = 4.06TNIVLCKK10 pKa = 10.45CNEE13 pKa = 3.95DD14 pKa = 3.48VFLEE18 pKa = 4.46LVQSEE23 pKa = 4.59QVFGHH28 pKa = 6.6PDD30 pKa = 3.33EE31 pKa = 4.95FVMHH35 pKa = 6.84IADD38 pKa = 4.35FCSLSDD44 pKa = 3.95DD45 pKa = 3.37MKK47 pKa = 11.41EE48 pKa = 4.28EE49 pKa = 4.05IEE51 pKa = 5.07HH52 pKa = 6.31EE53 pKa = 4.24YY54 pKa = 10.68LYY56 pKa = 11.27KK57 pKa = 10.52KK58 pKa = 10.63

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2ANN0|A0A1B2ANN0_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage PH1 OX=1874540 PE=4 SV=1
MM1 pKa = 8.01RR2 pKa = 11.84DD3 pKa = 3.29LVVIGLRR10 pKa = 11.84SASRR14 pKa = 11.84EE15 pKa = 3.99EE16 pKa = 4.11IKK18 pKa = 10.97ALTIEE23 pKa = 4.36WQTKK27 pKa = 9.37GYY29 pKa = 9.17IVKK32 pKa = 10.18HH33 pKa = 4.91KK34 pKa = 10.34RR35 pKa = 11.84FYY37 pKa = 11.08KK38 pKa = 10.52SGLPLPNNGVLQCTTKK54 pKa = 10.92

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13294

54

1299

246.2

27.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.252 ± 0.508

0.978 ± 0.146

6.289 ± 0.161

7.274 ± 0.235

3.799 ± 0.2

6.454 ± 0.284

1.843 ± 0.166

5.363 ± 0.193

6.762 ± 0.304

8.598 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.655 ± 0.168

4.98 ± 0.155

3.423 ± 0.178

4.1 ± 0.321

4.619 ± 0.266

6.386 ± 0.387

6.356 ± 0.254

6.499 ± 0.256

1.309 ± 0.124

4.062 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski