Salmonella phage STP03
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U9HYR6|A0A1U9HYR6_9CAUD Tail protein OS=Salmonella phage STP03 OX=1914788 GN=STP03_014 PE=4 SV=1
MM1 pKa = 7.22 VVGPQGAAEE10 pKa = 4.86 CIRR13 pKa = 11.84 FVDD16 pKa = 3.75 EE17 pKa = 4.18 ATIDD21 pKa = 4.23 FYY23 pKa = 11.52 DD24 pKa = 3.72 STGNWPYY31 pKa = 11.2 DD32 pKa = 3.4 CQEE35 pKa = 3.91 IYY37 pKa = 11.03 GGIASAIVRR46 pKa = 11.84 KK47 pKa = 9.48 PSVPLDD53 pKa = 3.72 SEE55 pKa = 4.37 VVYY58 pKa = 11.37 YY59 pKa = 10.62 EE60 pKa = 4.52 DD61 pKa = 4.82 YY62 pKa = 11.5 KK63 pKa = 11.47 NALNKK68 pKa = 9.98 QEE70 pKa = 4.15 NKK72 pKa = 10.61
Molecular weight: 8.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.996
IPC2_protein 4.291
IPC_protein 4.151
Toseland 3.973
ProMoST 4.291
Dawson 4.113
Bjellqvist 4.279
Wikipedia 4.024
Rodwell 3.986
Grimsley 3.884
Solomon 4.101
Lehninger 4.062
Nozaki 4.24
DTASelect 4.406
Thurlkill 4.012
EMBOSS 4.037
Sillero 4.266
Patrickios 0.846
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.163
Protein with the highest isoelectric point:
>tr|A0A1U9HZB3|A0A1U9HZB3_9CAUD Uncharacterized protein OS=Salmonella phage STP03 OX=1914788 GN=STP03_060 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.34 PNDD5 pKa = 3.45 LVTWTGRR12 pKa = 11.84 NGEE15 pKa = 4.19 TRR17 pKa = 11.84 HH18 pKa = 5.75 GKK20 pKa = 10.02 VISLQGIYY28 pKa = 10.73 ARR30 pKa = 11.84 VEE32 pKa = 3.4 WWRR35 pKa = 11.84 THH37 pKa = 4.93 AKK39 pKa = 8.99 KK40 pKa = 10.01 PRR42 pKa = 11.84 YY43 pKa = 8.3 FTMRR47 pKa = 11.84 LDD49 pKa = 3.62 KK50 pKa = 11.17 LIVKK54 pKa = 9.65
Molecular weight: 6.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.297
IPC2_protein 9.926
IPC_protein 10.672
Toseland 10.965
ProMoST 10.701
Dawson 11.023
Bjellqvist 10.716
Wikipedia 11.228
Rodwell 11.374
Grimsley 11.067
Solomon 11.155
Lehninger 11.125
Nozaki 10.921
DTASelect 10.716
Thurlkill 10.95
EMBOSS 11.359
Sillero 10.965
Patrickios 11.14
IPC_peptide 11.169
IPC2_peptide 9.428
IPC2.peptide.svr19 8.499
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
70
0
70
13263
41
852
189.5
21.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.772 ± 0.607
1.018 ± 0.129
6.039 ± 0.219
6.778 ± 0.373
3.732 ± 0.188
7.593 ± 0.283
1.734 ± 0.177
5.029 ± 0.197
5.987 ± 0.365
7.736 ± 0.291
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.586 ± 0.177
4.366 ± 0.271
3.921 ± 0.214
3.777 ± 0.317
5.67 ± 0.268
5.738 ± 0.285
6.266 ± 0.326
7.291 ± 0.31
1.47 ± 0.14
3.498 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here