Amino acid dipepetide frequency for Pseudomonas phage KTN4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.468AlaAla: 4.468 ± 0.306
0.586AlaCys: 0.586 ± 0.09
3.788AlaAsp: 3.788 ± 0.219
3.835AlaGlu: 3.835 ± 0.299
2.346AlaPhe: 2.346 ± 0.162
3.46AlaGly: 3.46 ± 0.267
1.173AlaHis: 1.173 ± 0.123
4.632AlaIle: 4.632 ± 0.204
3.964AlaLys: 3.964 ± 0.271
4.797AlaLeu: 4.797 ± 0.243
1.935AlaMet: 1.935 ± 0.138
3.729AlaAsn: 3.729 ± 0.219
1.994AlaPro: 1.994 ± 0.193
2.521AlaGln: 2.521 ± 0.211
2.697AlaArg: 2.697 ± 0.233
3.565AlaSer: 3.565 ± 0.259
4.257AlaThr: 4.257 ± 0.306
4.55AlaVal: 4.55 ± 0.261
0.68AlaTrp: 0.68 ± 0.098
2.615AlaTyr: 2.615 ± 0.154
0.0AlaXaa: 0.0 ± 0.0
Cys
0.504CysAla: 0.504 ± 0.075
0.141CysCys: 0.141 ± 0.043
0.586CysAsp: 0.586 ± 0.085
0.563CysGlu: 0.563 ± 0.084
0.34CysPhe: 0.34 ± 0.066
0.469CysGly: 0.469 ± 0.083
0.223CysHis: 0.223 ± 0.049
0.727CysIle: 0.727 ± 0.09
0.61CysLys: 0.61 ± 0.097
0.739CysLeu: 0.739 ± 0.108
0.246CysMet: 0.246 ± 0.052
0.668CysAsn: 0.668 ± 0.095
0.364CysPro: 0.364 ± 0.073
0.352CysGln: 0.352 ± 0.071
0.563CysArg: 0.563 ± 0.084
0.68CysSer: 0.68 ± 0.099
0.633CysThr: 0.633 ± 0.081
0.751CysVal: 0.751 ± 0.107
0.152CysTrp: 0.152 ± 0.045
0.422CysTyr: 0.422 ± 0.071
0.0CysXaa: 0.0 ± 0.0
Asp
3.53AspAla: 3.53 ± 0.216
0.493AspCys: 0.493 ± 0.076
4.574AspAsp: 4.574 ± 0.213
4.445AspGlu: 4.445 ± 0.229
2.639AspPhe: 2.639 ± 0.175
4.328AspGly: 4.328 ± 0.255
1.255AspHis: 1.255 ± 0.115
5.571AspIle: 5.571 ± 0.247
4.339AspLys: 4.339 ± 0.188
5.864AspLeu: 5.864 ± 0.237
1.736AspMet: 1.736 ± 0.183
3.706AspAsn: 3.706 ± 0.209
3.319AspPro: 3.319 ± 0.25
2.158AspGln: 2.158 ± 0.169
2.686AspArg: 2.686 ± 0.188
3.976AspSer: 3.976 ± 0.21
3.894AspThr: 3.894 ± 0.211
4.644AspVal: 4.644 ± 0.217
0.903AspTrp: 0.903 ± 0.104
3.002AspTyr: 3.002 ± 0.199
0.0AspXaa: 0.0 ± 0.0
Glu
3.999GluAla: 3.999 ± 0.267
0.668GluCys: 0.668 ± 0.089
3.894GluAsp: 3.894 ± 0.235
4.398GluGlu: 4.398 ± 0.401
2.838GluPhe: 2.838 ± 0.199
2.885GluGly: 2.885 ± 0.26
1.337GluHis: 1.337 ± 0.127
4.187GluIle: 4.187 ± 0.192
3.765GluLys: 3.765 ± 0.234
7.048GluLeu: 7.048 ± 0.292
1.982GluMet: 1.982 ± 0.158
3.225GluAsn: 3.225 ± 0.199
2.123GluPro: 2.123 ± 0.206
2.545GluGln: 2.545 ± 0.189
3.166GluArg: 3.166 ± 0.198
3.436GluSer: 3.436 ± 0.229
3.624GluThr: 3.624 ± 0.277
4.679GluVal: 4.679 ± 0.29
1.02GluTrp: 1.02 ± 0.102
3.014GluTyr: 3.014 ± 0.204
0.0GluXaa: 0.0 ± 0.0
Phe
1.97PheAla: 1.97 ± 0.158
0.352PheCys: 0.352 ± 0.061
3.155PheAsp: 3.155 ± 0.215
2.31PheGlu: 2.31 ± 0.158
1.665PhePhe: 1.665 ± 0.144
2.322PheGly: 2.322 ± 0.194
0.926PheHis: 0.926 ± 0.103
3.589PheIle: 3.589 ± 0.201
3.483PheLys: 3.483 ± 0.2
2.779PheLeu: 2.779 ± 0.191
1.091PheMet: 1.091 ± 0.109
3.495PheAsn: 3.495 ± 0.192
1.314PhePro: 1.314 ± 0.123
1.161PheGln: 1.161 ± 0.118
1.525PheArg: 1.525 ± 0.144
2.134PheSer: 2.134 ± 0.131
2.557PheThr: 2.557 ± 0.178
2.498PheVal: 2.498 ± 0.171
0.41PheTrp: 0.41 ± 0.08
1.63PheTyr: 1.63 ± 0.137
0.0PheXaa: 0.0 ± 0.0
Gly
3.037GlyAla: 3.037 ± 0.272
0.528GlyCys: 0.528 ± 0.081
3.8GlyAsp: 3.8 ± 0.236
3.6GlyGlu: 3.6 ± 0.285
2.521GlyPhe: 2.521 ± 0.182
3.202GlyGly: 3.202 ± 0.261
0.997GlyHis: 0.997 ± 0.119
4.034GlyIle: 4.034 ± 0.218
4.457GlyLys: 4.457 ± 0.289
4.457GlyLeu: 4.457 ± 0.226
1.478GlyMet: 1.478 ± 0.12
3.413GlyAsn: 3.413 ± 0.212
1.853GlyPro: 1.853 ± 0.346
1.841GlyGln: 1.841 ± 0.2
2.803GlyArg: 2.803 ± 0.193
3.213GlySer: 3.213 ± 0.216
3.448GlyThr: 3.448 ± 0.296
3.929GlyVal: 3.929 ± 0.214
0.833GlyTrp: 0.833 ± 0.09
2.838GlyTyr: 2.838 ± 0.172
0.0GlyXaa: 0.0 ± 0.0
His
1.055HisAla: 1.055 ± 0.111
0.223HisCys: 0.223 ± 0.048
1.185HisAsp: 1.185 ± 0.128
1.255HisGlu: 1.255 ± 0.127
0.915HisPhe: 0.915 ± 0.135
1.22HisGly: 1.22 ± 0.116
0.645HisHis: 0.645 ± 0.093
1.501HisIle: 1.501 ± 0.129
1.267HisLys: 1.267 ± 0.13
1.712HisLeu: 1.712 ± 0.127
0.457HisMet: 0.457 ± 0.079
1.044HisAsn: 1.044 ± 0.129
1.032HisPro: 1.032 ± 0.134
0.774HisGln: 0.774 ± 0.081
1.149HisArg: 1.149 ± 0.114
1.185HisSer: 1.185 ± 0.136
1.055HisThr: 1.055 ± 0.111
1.325HisVal: 1.325 ± 0.119
0.364HisTrp: 0.364 ± 0.066
0.938HisTyr: 0.938 ± 0.111
0.0HisXaa: 0.0 ± 0.0
Ile
4.457IleAla: 4.457 ± 0.207
0.821IleCys: 0.821 ± 0.098
5.993IleAsp: 5.993 ± 0.266
5.219IleGlu: 5.219 ± 0.271
2.041IlePhe: 2.041 ± 0.171
4.163IleGly: 4.163 ± 0.232
1.583IleHis: 1.583 ± 0.123
4.996IleIle: 4.996 ± 0.269
5.125IleLys: 5.125 ± 0.245
5.465IleLeu: 5.465 ± 0.276
1.185IleMet: 1.185 ± 0.121
5.266IleAsn: 5.266 ± 0.24
3.589IlePro: 3.589 ± 0.22
2.486IleGln: 2.486 ± 0.152
3.718IleArg: 3.718 ± 0.22
5.031IleSer: 5.031 ± 0.284
4.914IleThr: 4.914 ± 0.253
4.222IleVal: 4.222 ± 0.21
0.786IleTrp: 0.786 ± 0.091
2.662IleTyr: 2.662 ± 0.185
0.0IleXaa: 0.0 ± 0.0
Lys
4.574LysAla: 4.574 ± 0.328
0.563LysCys: 0.563 ± 0.094
4.269LysAsp: 4.269 ± 0.184
5.184LysGlu: 5.184 ± 0.305
2.486LysPhe: 2.486 ± 0.156
2.932LysGly: 2.932 ± 0.237
1.419LysHis: 1.419 ± 0.156
4.292LysIle: 4.292 ± 0.229
3.788LysLys: 3.788 ± 0.227
6.814LysLeu: 6.814 ± 0.335
2.123LysMet: 2.123 ± 0.152
3.378LysAsn: 3.378 ± 0.238
2.744LysPro: 2.744 ± 0.177
2.287LysGln: 2.287 ± 0.18
2.627LysArg: 2.627 ± 0.192
3.155LysSer: 3.155 ± 0.186
3.706LysThr: 3.706 ± 0.23
4.515LysVal: 4.515 ± 0.196
0.985LysTrp: 0.985 ± 0.106
2.991LysTyr: 2.991 ± 0.181
0.0LysXaa: 0.0 ± 0.0
Leu
5.688LeuAla: 5.688 ± 0.246
0.715LeuCys: 0.715 ± 0.097
5.465LeuAsp: 5.465 ± 0.268
5.172LeuGlu: 5.172 ± 0.256
3.378LeuPhe: 3.378 ± 0.181
4.48LeuGly: 4.48 ± 0.264
1.689LeuHis: 1.689 ± 0.138
6.075LeuIle: 6.075 ± 0.296
5.641LeuLys: 5.641 ± 0.248
6.532LeuLeu: 6.532 ± 0.303
2.31LeuMet: 2.31 ± 0.158
5.535LeuAsn: 5.535 ± 0.228
3.554LeuPro: 3.554 ± 0.201
2.416LeuGln: 2.416 ± 0.134
4.058LeuArg: 4.058 ± 0.241
5.594LeuSer: 5.594 ± 0.255
6.028LeuThr: 6.028 ± 0.24
5.946LeuVal: 5.946 ± 0.229
0.833LeuTrp: 0.833 ± 0.105
3.26LeuTyr: 3.26 ± 0.233
0.0LeuXaa: 0.0 ± 0.0
Met
2.146MetAla: 2.146 ± 0.173
0.399MetCys: 0.399 ± 0.067
1.712MetAsp: 1.712 ± 0.13
1.419MetGlu: 1.419 ± 0.128
1.337MetPhe: 1.337 ± 0.128
1.536MetGly: 1.536 ± 0.152
0.657MetHis: 0.657 ± 0.099
1.56MetIle: 1.56 ± 0.139
1.255MetLys: 1.255 ± 0.104
2.123MetLeu: 2.123 ± 0.147
0.821MetMet: 0.821 ± 0.108
1.501MetAsn: 1.501 ± 0.135
0.88MetPro: 0.88 ± 0.106
0.997MetGln: 0.997 ± 0.119
1.314MetArg: 1.314 ± 0.121
2.076MetSer: 2.076 ± 0.138
1.654MetThr: 1.654 ± 0.126
1.865MetVal: 1.865 ± 0.162
0.235MetTrp: 0.235 ± 0.054
1.231MetTyr: 1.231 ± 0.116
0.0MetXaa: 0.0 ± 0.0
Asn
3.53AsnAla: 3.53 ± 0.245
0.446AsnCys: 0.446 ± 0.071
3.835AsnAsp: 3.835 ± 0.208
3.765AsnGlu: 3.765 ± 0.217
2.158AsnPhe: 2.158 ± 0.143
4.41AsnGly: 4.41 ± 0.23
1.29AsnHis: 1.29 ± 0.128
4.867AsnIle: 4.867 ± 0.252
4.234AsnLys: 4.234 ± 0.224
4.316AsnLeu: 4.316 ± 0.257
1.677AsnMet: 1.677 ± 0.143
4.058AsnAsn: 4.058 ± 0.255
3.166AsnPro: 3.166 ± 0.192
2.181AsnGln: 2.181 ± 0.155
2.908AsnArg: 2.908 ± 0.158
3.671AsnSer: 3.671 ± 0.218
4.023AsnThr: 4.023 ± 0.213
3.46AsnVal: 3.46 ± 0.211
0.926AsnTrp: 0.926 ± 0.109
2.615AsnTyr: 2.615 ± 0.186
0.0AsnXaa: 0.0 ± 0.0
Pro
2.533ProAla: 2.533 ± 0.185
0.328ProCys: 0.328 ± 0.056
3.037ProAsp: 3.037 ± 0.214
2.885ProGlu: 2.885 ± 0.292
1.935ProPhe: 1.935 ± 0.135
2.521ProGly: 2.521 ± 0.236
0.915ProHis: 0.915 ± 0.1
2.557ProIle: 2.557 ± 0.166
2.217ProLys: 2.217 ± 0.165
3.026ProLeu: 3.026 ± 0.233
0.891ProMet: 0.891 ± 0.084
2.428ProAsn: 2.428 ± 0.184
1.208ProPro: 1.208 ± 0.127
1.161ProGln: 1.161 ± 0.157
1.642ProArg: 1.642 ± 0.138
2.498ProSer: 2.498 ± 0.173
3.002ProThr: 3.002 ± 0.195
3.366ProVal: 3.366 ± 0.184
0.34ProTrp: 0.34 ± 0.056
1.607ProTyr: 1.607 ± 0.139
0.0ProXaa: 0.0 ± 0.0
Gln
2.357GlnAla: 2.357 ± 0.221
0.375GlnCys: 0.375 ± 0.068
1.747GlnAsp: 1.747 ± 0.136
1.865GlnGlu: 1.865 ± 0.16
1.654GlnPhe: 1.654 ± 0.146
1.771GlnGly: 1.771 ± 0.313
0.692GlnHis: 0.692 ± 0.084
2.463GlnIle: 2.463 ± 0.169
1.959GlnLys: 1.959 ± 0.144
3.6GlnLeu: 3.6 ± 0.184
0.926GlnMet: 0.926 ± 0.105
1.689GlnAsn: 1.689 ± 0.148
1.384GlnPro: 1.384 ± 0.137
1.689GlnGln: 1.689 ± 0.169
1.97GlnArg: 1.97 ± 0.178
1.818GlnSer: 1.818 ± 0.148
1.724GlnThr: 1.724 ± 0.123
2.381GlnVal: 2.381 ± 0.176
0.586GlnTrp: 0.586 ± 0.084
1.489GlnTyr: 1.489 ± 0.126
0.0GlnXaa: 0.0 ± 0.0
Arg
2.908ArgAla: 2.908 ± 0.212
0.469ArgCys: 0.469 ± 0.071
3.143ArgAsp: 3.143 ± 0.267
2.744ArgGlu: 2.744 ± 0.19
1.935ArgPhe: 1.935 ± 0.156
2.615ArgGly: 2.615 ± 0.173
0.797ArgHis: 0.797 ± 0.095
3.401ArgIle: 3.401 ± 0.219
3.002ArgLys: 3.002 ± 0.182
4.855ArgLeu: 4.855 ± 0.276
1.255ArgMet: 1.255 ± 0.122
2.873ArgAsn: 2.873 ± 0.199
1.677ArgPro: 1.677 ± 0.146
1.536ArgGln: 1.536 ± 0.132
2.428ArgArg: 2.428 ± 0.179
2.568ArgSer: 2.568 ± 0.207
2.346ArgThr: 2.346 ± 0.135
3.225ArgVal: 3.225 ± 0.203
0.645ArgTrp: 0.645 ± 0.096
2.193ArgTyr: 2.193 ± 0.159
0.0ArgXaa: 0.0 ± 0.0
Ser
3.647SerAla: 3.647 ± 0.227
0.575SerCys: 0.575 ± 0.083
3.87SerAsp: 3.87 ± 0.211
3.589SerGlu: 3.589 ± 0.214
2.791SerPhe: 2.791 ± 0.204
3.448SerGly: 3.448 ± 0.218
1.055SerHis: 1.055 ± 0.109
4.961SerIle: 4.961 ± 0.234
3.683SerLys: 3.683 ± 0.197
5.184SerLeu: 5.184 ± 0.272
1.677SerMet: 1.677 ± 0.142
3.882SerAsn: 3.882 ± 0.216
2.158SerPro: 2.158 ± 0.161
1.97SerGln: 1.97 ± 0.156
2.756SerArg: 2.756 ± 0.173
3.894SerSer: 3.894 ± 0.255
3.858SerThr: 3.858 ± 0.208
4.292SerVal: 4.292 ± 0.224
0.844SerTrp: 0.844 ± 0.111
2.521SerTyr: 2.521 ± 0.18
0.0SerXaa: 0.0 ± 0.0
Thr
4.245ThrAla: 4.245 ± 0.32
0.692ThrCys: 0.692 ± 0.098
4.21ThrAsp: 4.21 ± 0.21
4.21ThrGlu: 4.21 ± 0.245
2.475ThrPhe: 2.475 ± 0.171
4.374ThrGly: 4.374 ± 0.269
1.185ThrHis: 1.185 ± 0.138
4.527ThrIle: 4.527 ± 0.228
3.765ThrLys: 3.765 ± 0.231
4.761ThrLeu: 4.761 ± 0.266
1.407ThrMet: 1.407 ± 0.123
3.577ThrAsn: 3.577 ± 0.258
2.92ThrPro: 2.92 ± 0.2
1.994ThrGln: 1.994 ± 0.149
2.615ThrArg: 2.615 ± 0.178
3.835ThrSer: 3.835 ± 0.227
4.175ThrThr: 4.175 ± 0.237
4.656ThrVal: 4.656 ± 0.218
0.844ThrTrp: 0.844 ± 0.1
2.686ThrTyr: 2.686 ± 0.171
0.0ThrXaa: 0.0 ± 0.0
Val
3.952ValAla: 3.952 ± 0.305
0.727ValCys: 0.727 ± 0.093
5.547ValAsp: 5.547 ± 0.231
4.457ValGlu: 4.457 ± 0.308
2.58ValPhe: 2.58 ± 0.183
3.225ValGly: 3.225 ± 0.217
1.091ValHis: 1.091 ± 0.11
5.618ValIle: 5.618 ± 0.283
4.726ValLys: 4.726 ± 0.239
5.102ValLeu: 5.102 ± 0.214
2.064ValMet: 2.064 ± 0.166
4.574ValAsn: 4.574 ± 0.215
2.428ValPro: 2.428 ± 0.177
1.947ValGln: 1.947 ± 0.14
3.049ValArg: 3.049 ± 0.186
4.668ValSer: 4.668 ± 0.222
4.691ValThr: 4.691 ± 0.266
4.574ValVal: 4.574 ± 0.276
0.68ValTrp: 0.68 ± 0.084
3.049ValTyr: 3.049 ± 0.186
0.0ValXaa: 0.0 ± 0.0
Trp
0.668TrpAla: 0.668 ± 0.089
0.164TrpCys: 0.164 ± 0.043
0.762TrpAsp: 0.762 ± 0.102
0.844TrpGlu: 0.844 ± 0.08
0.61TrpPhe: 0.61 ± 0.075
0.598TrpGly: 0.598 ± 0.091
0.305TrpHis: 0.305 ± 0.06
0.962TrpIle: 0.962 ± 0.087
0.833TrpLys: 0.833 ± 0.096
1.149TrpLeu: 1.149 ± 0.119
0.352TrpMet: 0.352 ± 0.075
0.739TrpAsn: 0.739 ± 0.095
0.446TrpPro: 0.446 ± 0.067
0.399TrpGln: 0.399 ± 0.058
0.575TrpArg: 0.575 ± 0.079
0.868TrpSer: 0.868 ± 0.105
0.727TrpThr: 0.727 ± 0.102
1.102TrpVal: 1.102 ± 0.131
0.188TrpTrp: 0.188 ± 0.05
0.586TrpTyr: 0.586 ± 0.087
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.322TyrAla: 2.322 ± 0.192
0.469TyrCys: 0.469 ± 0.072
2.486TyrAsp: 2.486 ± 0.176
2.076TyrGlu: 2.076 ± 0.147
1.724TyrPhe: 1.724 ± 0.146
2.322TyrGly: 2.322 ± 0.146
1.067TyrHis: 1.067 ± 0.117
3.202TyrIle: 3.202 ± 0.185
2.721TyrLys: 2.721 ± 0.162
4.046TyrLeu: 4.046 ± 0.229
1.091TyrMet: 1.091 ± 0.105
2.756TyrAsn: 2.756 ± 0.179
1.959TyrPro: 1.959 ± 0.158
1.724TyrGln: 1.724 ± 0.138
2.369TyrArg: 2.369 ± 0.159
2.791TyrSer: 2.791 ± 0.201
2.838TyrThr: 2.838 ± 0.186
2.768TyrVal: 2.768 ± 0.214
0.622TyrTrp: 0.622 ± 0.086
2.017TyrTyr: 2.017 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 368 proteins (85269 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski