Liberibacter phage HHCA1-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L2K1T2|A0A1L2K1T2_9CAUD Uncharacterized protein OS=Liberibacter phage HHCA1-2 OX=1903185 GN=PHHCA_gp39 PE=4 SV=1
MM1 pKa = 7.48GALKK5 pKa = 10.6NHH7 pKa = 6.01FHH9 pKa = 7.93DD10 pKa = 5.19EE11 pKa = 4.16INEE14 pKa = 3.7NFYY17 pKa = 11.02FHH19 pKa = 6.71SHH21 pKa = 6.94PNADD25 pKa = 3.54PDD27 pKa = 3.9ISIEE31 pKa = 4.02MQISEE36 pKa = 4.21NQRR39 pKa = 11.84YY40 pKa = 9.2LDD42 pKa = 4.37EE43 pKa = 5.99EE44 pKa = 4.27ISQCNAVVDD53 pKa = 3.79VFKK56 pKa = 11.01RR57 pKa = 11.84SDD59 pKa = 3.32STILDD64 pKa = 3.6KK65 pKa = 11.4LDD67 pKa = 4.08AVDD70 pKa = 5.19DD71 pKa = 4.32LKK73 pKa = 10.54TYY75 pKa = 10.69ISLLQATAKK84 pKa = 10.16NLKK87 pKa = 10.12SLLKK91 pKa = 10.06EE92 pKa = 3.88YY93 pKa = 10.14WEE95 pKa = 4.41EE96 pKa = 4.11SLDD99 pKa = 4.21GEE101 pKa = 4.55DD102 pKa = 4.66DD103 pKa = 3.84EE104 pKa = 6.1EE105 pKa = 5.64IYY107 pKa = 10.58EE108 pKa = 5.08HH109 pKa = 7.59PDD111 pKa = 2.95QEE113 pKa = 4.6HH114 pKa = 6.7RR115 pKa = 11.84EE116 pKa = 4.1DD117 pKa = 4.28YY118 pKa = 10.37YY119 pKa = 11.83ANQII123 pKa = 3.68

Molecular weight: 14.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L2K1H9|A0A1L2K1H9_9CAUD Uncharacterized protein OS=Liberibacter phage HHCA1-2 OX=1903185 GN=PHHCA_gp17 PE=4 SV=1
MM1 pKa = 7.14EE2 pKa = 5.59RR3 pKa = 11.84KK4 pKa = 9.76VLTPEE9 pKa = 3.28EE10 pKa = 4.09RR11 pKa = 11.84MLCRR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 3.85YY18 pKa = 10.42KK19 pKa = 9.68RR20 pKa = 11.84RR21 pKa = 11.84YY22 pKa = 7.74YY23 pKa = 10.85LKK25 pKa = 10.55NRR27 pKa = 11.84DD28 pKa = 3.68KK29 pKa = 10.67ILEE32 pKa = 4.0RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84YY38 pKa = 9.79LKK40 pKa = 10.94NKK42 pKa = 9.49DD43 pKa = 2.78KK44 pKa = 10.92CRR46 pKa = 11.84EE47 pKa = 3.9YY48 pKa = 10.56RR49 pKa = 11.84HH50 pKa = 6.14QYY52 pKa = 8.37YY53 pKa = 10.72LKK55 pKa = 10.91NKK57 pKa = 10.04DD58 pKa = 2.91KK59 pKa = 10.37YY60 pKa = 9.77RR61 pKa = 11.84EE62 pKa = 3.99YY63 pKa = 10.89KK64 pKa = 9.23RR65 pKa = 11.84RR66 pKa = 11.84YY67 pKa = 7.54YY68 pKa = 10.88LKK70 pKa = 10.43NRR72 pKa = 11.84DD73 pKa = 3.28KK74 pKa = 10.56MRR76 pKa = 11.84EE77 pKa = 3.85KK78 pKa = 10.72ARR80 pKa = 11.84QSYY83 pKa = 9.71RR84 pKa = 11.84KK85 pKa = 9.71LYY87 pKa = 11.12SKK89 pKa = 10.49DD90 pKa = 2.72SWIAPEE96 pKa = 4.72EE97 pKa = 3.89PMGMTKK103 pKa = 10.59AEE105 pKa = 3.89IEE107 pKa = 3.96ALEE110 pKa = 4.15RR111 pKa = 11.84EE112 pKa = 4.31IARR115 pKa = 11.84LKK117 pKa = 10.67AKK119 pKa = 10.11PIEE122 pKa = 3.76EE123 pKa = 5.01LIYY126 pKa = 10.69KK127 pKa = 10.48RR128 pKa = 11.84SS129 pKa = 3.27

Molecular weight: 16.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

11445

34

809

260.1

29.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.23 ± 0.311

1.18 ± 0.164

6.09 ± 0.2

7.497 ± 0.338

4.176 ± 0.251

6.518 ± 0.683

2.01 ± 0.184

6.396 ± 0.342

8.257 ± 0.318

9.078 ± 0.373

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.957 ± 0.136

3.871 ± 0.2

3.81 ± 0.349

4.605 ± 0.462

5.513 ± 0.287

6.876 ± 0.312

5.557 ± 0.224

5.74 ± 0.301

1.512 ± 0.132

3.128 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski