Barrientosiimonas humi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermacoccaceae; Barrientosiimonas

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3329 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A542XBL3|A0A542XBL3_9MICO tRNA G18 (Ribose-2'-O)-methylase SpoU OS=Barrientosiimonas humi OX=999931 GN=FB554_1371 PE=4 SV=1
MM1 pKa = 7.9SDD3 pKa = 3.73LEE5 pKa = 4.29YY6 pKa = 10.12PANLRR11 pKa = 11.84YY12 pKa = 8.5TSDD15 pKa = 3.83HH16 pKa = 6.15EE17 pKa = 4.39WVADD21 pKa = 4.01RR22 pKa = 11.84GEE24 pKa = 4.48GIVRR28 pKa = 11.84VGVTAYY34 pKa = 10.47AQDD37 pKa = 3.46ALGDD41 pKa = 3.79VVYY44 pKa = 11.18VSLPNKK50 pKa = 9.82GDD52 pKa = 3.3TVAAGDD58 pKa = 3.56SCGEE62 pKa = 4.11VEE64 pKa = 4.44STKK67 pKa = 10.7SVSDD71 pKa = 4.28LYY73 pKa = 11.5SPLDD77 pKa = 3.74GEE79 pKa = 4.65VTTVNDD85 pKa = 3.98ALEE88 pKa = 4.4SSPEE92 pKa = 4.03LVNSDD97 pKa = 3.98PYY99 pKa = 11.37GDD101 pKa = 2.96GWMYY105 pKa = 10.8EE106 pKa = 3.94MSVADD111 pKa = 3.49SAAIGEE117 pKa = 4.59LMDD120 pKa = 4.43ADD122 pKa = 4.76AYY124 pKa = 10.74RR125 pKa = 11.84SSLAA129 pKa = 3.62

Molecular weight: 13.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A542X8E0|A0A542X8E0_9MICO Uncharacterized protein OS=Barrientosiimonas humi OX=999931 GN=FB554_0232 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3329

0

3329

1100107

29

1847

330.5

35.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.977 ± 0.065

0.664 ± 0.009

6.45 ± 0.036

5.714 ± 0.041

2.646 ± 0.024

9.327 ± 0.044

2.01 ± 0.02

3.223 ± 0.032

1.916 ± 0.032

10.293 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.822 ± 0.017

1.644 ± 0.02

5.948 ± 0.036

3.319 ± 0.022

8.031 ± 0.044

5.289 ± 0.027

6.085 ± 0.029

9.261 ± 0.038

1.517 ± 0.018

1.863 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski