Micromonosporaceae bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; unclassified Micromonosporaceae

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4518 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N7GNQ5|A0A6N7GNQ5_9ACTN Chorismate mutase OS=Micromonosporaceae bacterium OX=1873464 GN=GEV12_21365 PE=4 SV=1
MM1 pKa = 7.71HH2 pKa = 8.14DD3 pKa = 3.61EE4 pKa = 5.4PIDD7 pKa = 4.02PFQGDD12 pKa = 3.56PADD15 pKa = 4.25PSSEE19 pKa = 3.99LDD21 pKa = 3.65YY22 pKa = 10.92PADD25 pKa = 4.54DD26 pKa = 4.97LLSDD30 pKa = 4.58PLSEE34 pKa = 5.43ADD36 pKa = 3.83RR37 pKa = 11.84QDD39 pKa = 3.58VLEE42 pKa = 5.05DD43 pKa = 3.51LADD46 pKa = 3.97LEE48 pKa = 5.03VYY50 pKa = 9.87QALLGPIGIRR60 pKa = 11.84GLVIEE65 pKa = 5.13CDD67 pKa = 3.66EE68 pKa = 4.38CHH70 pKa = 6.23EE71 pKa = 3.98PHH73 pKa = 6.77YY74 pKa = 10.68FDD76 pKa = 4.51WDD78 pKa = 3.86LLRR81 pKa = 11.84GNLRR85 pKa = 11.84QLLDD89 pKa = 3.52SGVPRR94 pKa = 11.84VHH96 pKa = 7.08EE97 pKa = 4.32PACDD101 pKa = 3.86PDD103 pKa = 3.65PEE105 pKa = 5.33RR106 pKa = 11.84YY107 pKa = 9.98VSWDD111 pKa = 3.41YY112 pKa = 11.94ARR114 pKa = 11.84GYY116 pKa = 10.38YY117 pKa = 10.34DD118 pKa = 3.41GVKK121 pKa = 9.18NTVADD126 pKa = 4.1DD127 pKa = 3.54

Molecular weight: 14.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N7G7Z3|A0A6N7G7Z3_9ACTN Uncharacterized protein OS=Micromonosporaceae bacterium OX=1873464 GN=GEV12_02960 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 7.46KK15 pKa = 7.68THH17 pKa = 5.01GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILRR33 pKa = 11.84ARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.49GRR40 pKa = 11.84TRR42 pKa = 11.84LSAA45 pKa = 3.71

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4518

0

4518

1457543

31

3167

322.6

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.307 ± 0.061

0.768 ± 0.011

5.939 ± 0.031

5.306 ± 0.028

2.567 ± 0.021

9.717 ± 0.038

2.125 ± 0.018

2.991 ± 0.023

1.216 ± 0.019

10.674 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.534 ± 0.014

1.525 ± 0.018

6.698 ± 0.036

3.073 ± 0.02

8.578 ± 0.046

4.594 ± 0.028

5.766 ± 0.027

9.11 ± 0.035

1.541 ± 0.013

1.97 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski