Thermoplasmatales archaeon A-plasma 
Average proteome isoelectric point is 6.7 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 2276 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|T0M4U8|T0M4U8_9ARCH Major facilitator superfamily MFS_1 OS=Thermoplasmatales archaeon A-plasma OX=667135 GN=AMDU1_APLC00016G0024 PE=4 SV=1 
MM1 pKa = 7.24  TLKK4 pKa = 10.7  LDD6 pKa = 3.42  ARR8 pKa = 11.84  LLYY11 pKa = 10.51  EE12 pKa = 4.37  IMSEE16 pKa = 3.9  FHH18 pKa = 6.05  TLGDD22 pKa = 3.89  EE23 pKa = 4.4  EE24 pKa = 5.11  YY25 pKa = 10.36  GGQGTFQEE33 pKa = 5.18  AILVGYY39 pKa = 9.98  IYY41 pKa = 11.18  GLLTEE46 pKa = 4.87  NPSSVMTLDD55 pKa = 3.54  SGTHH59 pKa = 5.38  KK60 pKa = 10.41  VFSYY64 pKa = 10.77  GGYY67 pKa = 10.16  SYY69 pKa = 10.48  IIWFDD74 pKa = 3.55  EE75 pKa = 4.11  IYY77 pKa = 10.53  AYY79 pKa = 10.48  AEE81 pKa = 4.2  DD82 pKa = 5.69  DD83 pKa = 3.73  EE84 pKa = 5.63  DD85 pKa = 3.7  EE86 pKa = 5.12  KK87 pKa = 11.41  EE88 pKa = 4.24  PHH90 pKa = 6.51  DD91 pKa = 3.73  QATEE95 pKa = 3.96  TFEE98 pKa = 4.75  VKK100 pKa = 10.2  IEE102 pKa = 4.21  DD103 pKa = 3.66  VSEE106 pKa = 4.62  DD107 pKa = 3.93  DD108 pKa = 5.62  DD109 pKa = 6.15  RR110 pKa = 11.84  EE111 pKa = 4.36  GDD113 pKa = 3.59  DD114 pKa = 5.44  DD115 pKa = 4.4  EE116 pKa = 6.6  AVLLPVAVEE125 pKa = 4.6  GPYY128 pKa = 9.77  TADD131 pKa = 4.66  DD132 pKa = 3.78  IKK134 pKa = 11.51  DD135 pKa = 3.73  FLANGDD141 pKa = 3.9  LL142 pKa = 4.0   
 Molecular weight: 16.0 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.741 
IPC2_protein 3.681 
IPC_protein 3.668 
Toseland    3.465 
ProMoST     3.834 
Dawson      3.656 
Bjellqvist  3.808 
Wikipedia   3.579 
Rodwell     3.503 
Grimsley    3.376 
Solomon     3.643 
Lehninger   3.605 
Nozaki      3.77 
DTASelect   3.973 
Thurlkill   3.516 
EMBOSS      3.592 
Sillero     3.795 
Patrickios  0.731 
IPC_peptide 3.643 
IPC2_peptide  3.77 
IPC2.peptide.svr19  3.709 
 Protein with the highest isoelectric point: 
>tr|T0NAW7|T0NAW7_9ARCH ABC transporter ATP-binding protein OS=Thermoplasmatales archaeon A-plasma OX=667135 GN=AMDU1_APLC00044G0033 PE=4 SV=1 
MM1 pKa = 7.91  RR2 pKa = 11.84  GGDD5 pKa = 3.13  RR6 pKa = 11.84  TAKK9 pKa = 10.22  KK10 pKa = 9.93  RR11 pKa = 11.84  IKK13 pKa = 9.96  RR14 pKa = 11.84  RR15 pKa = 11.84  KK16 pKa = 7.22  ATNAAKK22 pKa = 10.07  RR23 pKa = 11.84  RR24 pKa = 11.84  KK25 pKa = 7.87  PTRR28 pKa = 11.84  KK29 pKa = 9.32  PKK31 pKa = 10.36  GKK33 pKa = 8.59  KK34 pKa = 5.8  TRR36 pKa = 11.84  KK37 pKa = 7.15  RR38 pKa = 11.84  TGRR41 pKa = 11.84  RR42 pKa = 3.18   
 Molecular weight: 4.99 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.447 
IPC2_protein 10.979 
IPC_protein 12.544 
Toseland    12.72 
ProMoST     13.203 
Dawson      12.72 
Bjellqvist  12.705 
Wikipedia   13.188 
Rodwell     12.515 
Grimsley    12.749 
Solomon     13.203 
Lehninger   13.1 
Nozaki      12.72 
DTASelect   12.705 
Thurlkill   12.72 
EMBOSS      13.217 
Sillero     12.72 
Patrickios  12.237 
IPC_peptide 13.203 
IPC2_peptide  12.193 
IPC2.peptide.svr19  9.048 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        2276 
 
        
        0
 
        
        2276 
         
        587698
 
        24
 
        2121
 
        258.2
 
        28.78
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        6.973 ± 0.055
0.658 ± 0.016
 
        5.227 ± 0.05
6.048 ± 0.064
 
        4.642 ± 0.042
7.586 ± 0.05
 
        1.795 ± 0.02
8.059 ± 0.045
       
        5.293 ± 0.061
9.051 ± 0.055
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        3.154 ± 0.028
4.201 ± 0.052
 
        4.059 ± 0.035
2.522 ± 0.029
 
        5.568 ± 0.058
8.046 ± 0.066
 
        5.205 ± 0.043
7.092 ± 0.039
       
        0.929 ± 0.018
3.889 ± 0.04
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here