Thermoplasmatales archaeon A-plasma
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2276 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T0M4U8|T0M4U8_9ARCH Major facilitator superfamily MFS_1 OS=Thermoplasmatales archaeon A-plasma OX=667135 GN=AMDU1_APLC00016G0024 PE=4 SV=1
MM1 pKa = 7.24 TLKK4 pKa = 10.7 LDD6 pKa = 3.42 ARR8 pKa = 11.84 LLYY11 pKa = 10.51 EE12 pKa = 4.37 IMSEE16 pKa = 3.9 FHH18 pKa = 6.05 TLGDD22 pKa = 3.89 EE23 pKa = 4.4 EE24 pKa = 5.11 YY25 pKa = 10.36 GGQGTFQEE33 pKa = 5.18 AILVGYY39 pKa = 9.98 IYY41 pKa = 11.18 GLLTEE46 pKa = 4.87 NPSSVMTLDD55 pKa = 3.54 SGTHH59 pKa = 5.38 KK60 pKa = 10.41 VFSYY64 pKa = 10.77 GGYY67 pKa = 10.16 SYY69 pKa = 10.48 IIWFDD74 pKa = 3.55 EE75 pKa = 4.11 IYY77 pKa = 10.53 AYY79 pKa = 10.48 AEE81 pKa = 4.2 DD82 pKa = 5.69 DD83 pKa = 3.73 EE84 pKa = 5.63 DD85 pKa = 3.7 EE86 pKa = 5.12 KK87 pKa = 11.41 EE88 pKa = 4.24 PHH90 pKa = 6.51 DD91 pKa = 3.73 QATEE95 pKa = 3.96 TFEE98 pKa = 4.75 VKK100 pKa = 10.2 IEE102 pKa = 4.21 DD103 pKa = 3.66 VSEE106 pKa = 4.62 DD107 pKa = 3.93 DD108 pKa = 5.62 DD109 pKa = 6.15 RR110 pKa = 11.84 EE111 pKa = 4.36 GDD113 pKa = 3.59 DD114 pKa = 5.44 DD115 pKa = 4.4 EE116 pKa = 6.6 AVLLPVAVEE125 pKa = 4.6 GPYY128 pKa = 9.77 TADD131 pKa = 4.66 DD132 pKa = 3.78 IKK134 pKa = 11.51 DD135 pKa = 3.73 FLANGDD141 pKa = 3.9 LL142 pKa = 4.0
Molecular weight: 16.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.681
IPC_protein 3.668
Toseland 3.465
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.579
Rodwell 3.503
Grimsley 3.376
Solomon 3.643
Lehninger 3.605
Nozaki 3.77
DTASelect 3.973
Thurlkill 3.516
EMBOSS 3.592
Sillero 3.795
Patrickios 0.731
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.709
Protein with the highest isoelectric point:
>tr|T0NAW7|T0NAW7_9ARCH ABC transporter ATP-binding protein OS=Thermoplasmatales archaeon A-plasma OX=667135 GN=AMDU1_APLC00044G0033 PE=4 SV=1
MM1 pKa = 7.91 RR2 pKa = 11.84 GGDD5 pKa = 3.13 RR6 pKa = 11.84 TAKK9 pKa = 10.22 KK10 pKa = 9.93 RR11 pKa = 11.84 IKK13 pKa = 9.96 RR14 pKa = 11.84 RR15 pKa = 11.84 KK16 pKa = 7.22 ATNAAKK22 pKa = 10.07 RR23 pKa = 11.84 RR24 pKa = 11.84 KK25 pKa = 7.87 PTRR28 pKa = 11.84 KK29 pKa = 9.32 PKK31 pKa = 10.36 GKK33 pKa = 8.59 KK34 pKa = 5.8 TRR36 pKa = 11.84 KK37 pKa = 7.15 RR38 pKa = 11.84 TGRR41 pKa = 11.84 RR42 pKa = 3.18
Molecular weight: 4.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 10.979
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.515
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.237
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.048
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2276
0
2276
587698
24
2121
258.2
28.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.973 ± 0.055
0.658 ± 0.016
5.227 ± 0.05
6.048 ± 0.064
4.642 ± 0.042
7.586 ± 0.05
1.795 ± 0.02
8.059 ± 0.045
5.293 ± 0.061
9.051 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.154 ± 0.028
4.201 ± 0.052
4.059 ± 0.035
2.522 ± 0.029
5.568 ± 0.058
8.046 ± 0.066
5.205 ± 0.043
7.092 ± 0.039
0.929 ± 0.018
3.889 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here