Sulfitobacter mediterraneus
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3649 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A061SMD1|A0A061SMD1_9RHOB Transcriptional regulator OS=Sulfitobacter mediterraneus OX=83219 GN=C8N31_11453 PE=4 SV=1
MM1 pKa = 7.26 GVDD4 pKa = 4.2 AAVIWLRR11 pKa = 11.84 GFSLFAAMALNGPAAAQSPEE31 pKa = 3.74 EE32 pKa = 3.84 AQAYY36 pKa = 9.6 VEE38 pKa = 4.78 ANLISIFYY46 pKa = 10.15 HH47 pKa = 5.75 EE48 pKa = 5.56 LGHH51 pKa = 7.69 ALIDD55 pKa = 3.91 VMALPVFGQEE65 pKa = 3.62 EE66 pKa = 4.17 DD67 pKa = 3.83 AADD70 pKa = 3.63 TASILLIDD78 pKa = 4.42 ALFEE82 pKa = 4.34 EE83 pKa = 5.38 DD84 pKa = 3.45 TAQSIARR91 pKa = 11.84 DD92 pKa = 3.41 AAFGFLDD99 pKa = 3.73 EE100 pKa = 4.66 AAATEE105 pKa = 4.31 GEE107 pKa = 4.33 EE108 pKa = 4.0 PLWWDD113 pKa = 2.93 VHH115 pKa = 7.36 GPDD118 pKa = 5.01 LQRR121 pKa = 11.84 HH122 pKa = 5.07 YY123 pKa = 11.75 NLVCLFYY130 pKa = 10.99 GADD133 pKa = 3.47 PDD135 pKa = 4.12 LRR137 pKa = 11.84 ADD139 pKa = 3.44 IAADD143 pKa = 3.54 LDD145 pKa = 4.13 LPQDD149 pKa = 3.6 RR150 pKa = 11.84 AEE152 pKa = 4.29 SCAEE156 pKa = 3.94 EE157 pKa = 4.29 FDD159 pKa = 4.78 LALASWGPVLDD170 pKa = 6.53 DD171 pKa = 3.11 ITQNGGGASLVFEE184 pKa = 4.99 DD185 pKa = 5.41 AGASPLQDD193 pKa = 3.45 VIATEE198 pKa = 4.34 VAALNDD204 pKa = 4.73 LLTLPYY210 pKa = 9.77 PVTIRR215 pKa = 11.84 AEE217 pKa = 4.06 PCGEE221 pKa = 3.74 ANAFYY226 pKa = 10.84 DD227 pKa = 4.74 PADD230 pKa = 3.59 QTITMCLEE238 pKa = 4.29 LEE240 pKa = 4.05 SHH242 pKa = 6.24 LFEE245 pKa = 5.27 RR246 pKa = 11.84 AVQNIEE252 pKa = 3.69
Molecular weight: 27.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.63
IPC_protein 3.63
Toseland 3.427
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.795
Wikipedia 3.541
Rodwell 3.465
Grimsley 3.338
Solomon 3.605
Lehninger 3.567
Nozaki 3.732
DTASelect 3.935
Thurlkill 3.478
EMBOSS 3.554
Sillero 3.757
Patrickios 0.807
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.694
Protein with the highest isoelectric point:
>tr|A0A061SJG4|A0A061SJG4_9RHOB MFS transporter OS=Sulfitobacter mediterraneus OX=83219 GN=PM02_17180 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.34 SLSAA44 pKa = 3.83
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3649
0
3649
1135408
41
4535
311.2
33.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.097 ± 0.061
0.906 ± 0.013
6.362 ± 0.056
5.59 ± 0.03
3.798 ± 0.026
8.704 ± 0.048
2.102 ± 0.025
5.3 ± 0.03
3.489 ± 0.032
9.862 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.956 ± 0.024
2.82 ± 0.025
4.921 ± 0.028
3.54 ± 0.024
6.091 ± 0.044
5.154 ± 0.027
5.49 ± 0.036
7.187 ± 0.032
1.392 ± 0.017
2.239 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here