Salmonella phage 38
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 265 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N7C9G8|A0A0N7C9G8_9CAUD Uncharacterized protein OS=Salmonella phage 38 OX=1654891 GN=SP38_63 PE=4 SV=1
MM1 pKa = 7.74 IEE3 pKa = 4.0 YY4 pKa = 9.6 TPIQLGNGGGSSSSTITWVYY24 pKa = 10.94 NGGSAIGGEE33 pKa = 4.43 TEE35 pKa = 3.56 ITLDD39 pKa = 3.63 VVVDD43 pKa = 4.4 DD44 pKa = 5.65 VPAIDD49 pKa = 3.93 INGSRR54 pKa = 11.84 QYY56 pKa = 11.35 KK57 pKa = 10.04 NLGFTFDD64 pKa = 4.5 PLTSKK69 pKa = 8.84 ITLAQEE75 pKa = 4.32 LDD77 pKa = 3.76 TEE79 pKa = 4.58 DD80 pKa = 3.72 EE81 pKa = 4.43 VVVIINGTPNIYY93 pKa = 10.43 NQIDD97 pKa = 3.58 YY98 pKa = 9.01 TLRR101 pKa = 11.84 EE102 pKa = 4.13 VARR105 pKa = 11.84 VTNVKK110 pKa = 9.0 DD111 pKa = 3.49 TEE113 pKa = 4.33 VVYY116 pKa = 10.6 LVLVLCC122 pKa = 5.34
Molecular weight: 13.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.923
IPC_protein 3.846
Toseland 3.656
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.681
Grimsley 3.567
Solomon 3.821
Lehninger 3.77
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.973
Patrickios 1.888
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.866
Protein with the highest isoelectric point:
>tr|A0A0N7CEB2|A0A0N7CEB2_9CAUD Uncharacterized protein OS=Salmonella phage 38 OX=1654891 GN=SP38_159 PE=4 SV=1
MM1 pKa = 7.37 ACKK4 pKa = 9.41 TPEE7 pKa = 3.85 MKK9 pKa = 10.56 LVVSSKK15 pKa = 9.8 GRR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 SRR22 pKa = 11.84 NLITRR27 pKa = 11.84 LDD29 pKa = 3.63 ILRR32 pKa = 11.84 QKK34 pKa = 9.5 YY35 pKa = 7.98 QAHH38 pKa = 5.62 SVFTRR43 pKa = 11.84 IRR45 pKa = 11.84 NALKK49 pKa = 10.19 EE50 pKa = 3.98 GRR52 pKa = 11.84 TEE54 pKa = 4.12 LEE56 pKa = 3.91 LYY58 pKa = 10.03 RR59 pKa = 11.84 PNGSTRR65 pKa = 11.84 AYY67 pKa = 8.21 QTTDD71 pKa = 2.69 GLLEE75 pKa = 4.85 LIRR78 pKa = 11.84 LSGMTIEE85 pKa = 4.9 PRR87 pKa = 11.84 SSGTPLCSLYY97 pKa = 11.17 VIGNLGALL105 pKa = 3.84
Molecular weight: 11.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.736
IPC_protein 10.599
Toseland 10.584
ProMoST 10.335
Dawson 10.716
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.891
Grimsley 10.789
Solomon 10.833
Lehninger 10.789
Nozaki 10.584
DTASelect 10.452
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.657
Patrickios 10.628
IPC_peptide 10.833
IPC2_peptide 9.575
IPC2.peptide.svr19 8.458
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
265
0
265
44652
37
878
168.5
19.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.6 ± 0.179
1.187 ± 0.07
6.389 ± 0.12
6.519 ± 0.154
4.175 ± 0.1
6.578 ± 0.164
1.908 ± 0.093
6.286 ± 0.118
6.38 ± 0.145
8.051 ± 0.168
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.1 ± 0.095
5.162 ± 0.117
3.85 ± 0.111
3.787 ± 0.105
4.974 ± 0.126
6.454 ± 0.157
6.092 ± 0.173
7.14 ± 0.161
1.498 ± 0.073
3.87 ± 0.098
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here