Piscine myocarditis virus AL V-708
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E3W912|E3W912_9VIRU RNA-directed RNA polymerase OS=Piscine myocarditis virus AL V-708 OX=912320 PE=3 SV=1
MM1 pKa = 7.66 EE2 pKa = 6.22 PNTSVIATEE11 pKa = 4.01 QQQAAMRR18 pKa = 11.84 EE19 pKa = 4.41 VEE21 pKa = 4.29 AEE23 pKa = 3.5 AAARR27 pKa = 11.84 DD28 pKa = 3.8 EE29 pKa = 4.39 VVEE32 pKa = 4.8 KK33 pKa = 10.67 IAFAEE38 pKa = 4.48 GAMMVQTRR46 pKa = 11.84 RR47 pKa = 11.84 LPSGKK52 pKa = 9.96 SSVGGFLGEE61 pKa = 4.05 LAQNIRR67 pKa = 11.84 AMNRR71 pKa = 11.84 SLHH74 pKa = 6.23 TDD76 pKa = 2.82 TNMLTEE82 pKa = 4.11 GAMVDD87 pKa = 3.33 RR88 pKa = 11.84 ARR90 pKa = 11.84 AKK92 pKa = 7.48 VHH94 pKa = 6.15 KK95 pKa = 9.94 IIRR98 pKa = 11.84 EE99 pKa = 4.08 GNLDD103 pKa = 3.4 SRR105 pKa = 11.84 VFSNTGSNTMLSLWVPAVPGPPAVPEE131 pKa = 4.15 HH132 pKa = 6.92 WDD134 pKa = 3.43 VAPSWFVCRR143 pKa = 11.84 PGKK146 pKa = 10.37 KK147 pKa = 10.03 GGIKK151 pKa = 8.63 ITQSASMAALNPLFRR166 pKa = 11.84 GADD169 pKa = 3.29 VGPIGTAVRR178 pKa = 11.84 ADD180 pKa = 3.44 VNAFSMNAVLGALRR194 pKa = 11.84 AGGFNTEE201 pKa = 3.76 HH202 pKa = 6.68 SLVSFVEE209 pKa = 3.76 PLIRR213 pKa = 11.84 ILLMGVQTQDD223 pKa = 3.08 RR224 pKa = 11.84 GTSPWDD230 pKa = 3.15 WVGGMSSRR238 pKa = 11.84 IVNPLVFTTSGNFFPGGPNLRR259 pKa = 11.84 VWGANDD265 pKa = 3.22 TVARR269 pKa = 11.84 IVNVEE274 pKa = 3.83 DD275 pKa = 3.75 YY276 pKa = 9.78 MRR278 pKa = 11.84 EE279 pKa = 3.75 AAGEE283 pKa = 4.22 GRR285 pKa = 11.84 FDD287 pKa = 4.39 AGWGPEE293 pKa = 4.04 FWGGTGDD300 pKa = 3.79 DD301 pKa = 3.85 AVAVVPIRR309 pKa = 11.84 AVEE312 pKa = 4.09 AGLGEE317 pKa = 4.57 VNAGWTLAHH326 pKa = 6.36 MEE328 pKa = 4.33 YY329 pKa = 9.87 PVKK332 pKa = 10.77 VRR334 pKa = 11.84 LLDD337 pKa = 3.54 VDD339 pKa = 4.46 DD340 pKa = 4.01 RR341 pKa = 11.84 TIGPGGSLPLNANRR355 pKa = 11.84 EE356 pKa = 4.11 YY357 pKa = 9.54 TAAGATHH364 pKa = 6.4 VPGPYY369 pKa = 10.13 ARR371 pKa = 11.84 VLYY374 pKa = 10.5 VVVDD378 pKa = 4.38 QNADD382 pKa = 3.33 RR383 pKa = 11.84 CVGVRR388 pKa = 11.84 VQGQGAVIDD397 pKa = 3.88 VDD399 pKa = 3.56 PALNYY404 pKa = 10.48 VIGGADD410 pKa = 3.85 LGMLPLIQWSVGLGAEE426 pKa = 4.59 DD427 pKa = 3.66 MAQGSIAQTQRR438 pKa = 11.84 WVRR441 pKa = 11.84 MYY443 pKa = 11.13 GNEE446 pKa = 4.68 DD447 pKa = 3.17 DD448 pKa = 5.49 WEE450 pKa = 4.69 SAWHH454 pKa = 5.9 LVSSAYY460 pKa = 8.9 TVYY463 pKa = 10.78 SPAFRR468 pKa = 11.84 RR469 pKa = 11.84 SGVAVEE475 pKa = 4.44 GGFWAQPAAGAAPFPLGGLAGWVRR499 pKa = 11.84 YY500 pKa = 9.8 DD501 pKa = 3.47 NQARR505 pKa = 11.84 AAQVALCRR513 pKa = 11.84 EE514 pKa = 4.13 RR515 pKa = 11.84 ADD517 pKa = 3.51 MAEE520 pKa = 4.54 CPWGGYY526 pKa = 7.07 RR527 pKa = 11.84 EE528 pKa = 4.04 RR529 pKa = 11.84 GVRR532 pKa = 11.84 PGSVANWQYY541 pKa = 11.32 VRR543 pKa = 11.84 FDD545 pKa = 3.28 PTVAVGVAAHH555 pKa = 6.47 FWSVVKK561 pKa = 10.73 VMVAPVPDD569 pKa = 3.78 RR570 pKa = 11.84 AAALADD576 pKa = 3.76 MAWGKK581 pKa = 11.07 GKK583 pKa = 9.66 VQAMGEE589 pKa = 4.14 DD590 pKa = 4.19 VINGQMGQPEE600 pKa = 4.69 SMMRR604 pKa = 11.84 GVALNEE610 pKa = 4.07 NQGLAAATVRR620 pKa = 11.84 RR621 pKa = 11.84 VVGLEE626 pKa = 3.87 NEE628 pKa = 4.59 SMQTTHH634 pKa = 6.25 WSTTEE639 pKa = 3.66 VAMNGYY645 pKa = 9.34 YY646 pKa = 10.55 GRR648 pKa = 11.84 AGATAHH654 pKa = 6.63 HH655 pKa = 6.67 AAFPLSEE662 pKa = 4.39 GGTMRR667 pKa = 11.84 KK668 pKa = 9.79 RR669 pKa = 11.84 IPAIEE674 pKa = 3.84 MRR676 pKa = 11.84 EE677 pKa = 3.97 NGVEE681 pKa = 4.0 GDD683 pKa = 4.31 LMNDD687 pKa = 3.43 DD688 pKa = 5.37 LYY690 pKa = 11.61 SIGTAAGYY698 pKa = 9.76 LAVEE702 pKa = 4.79 GMAGAQGGIWDD713 pKa = 3.86 VVQYY717 pKa = 9.15 QLPGPDD723 pKa = 3.42 DD724 pKa = 3.6 EE725 pKa = 5.87 ARR727 pKa = 11.84 GVMNTVGAMGGWTRR741 pKa = 11.84 AVTPVDD747 pKa = 3.4 NVATMRR753 pKa = 11.84 DD754 pKa = 3.33 NGVEE758 pKa = 4.01 GEE760 pKa = 4.12 PCGIVMSLPTSGTAVVDD777 pKa = 4.04 RR778 pKa = 11.84 LANFGLPPARR788 pKa = 11.84 AEE790 pKa = 3.92 LRR792 pKa = 11.84 EE793 pKa = 4.14 VPFGGYY799 pKa = 7.64 QRR801 pKa = 11.84 SVTNTNHH808 pKa = 5.89 RR809 pKa = 11.84 VKK811 pKa = 10.85 VSVSGGRR818 pKa = 11.84 AVVQKK823 pKa = 10.92 GNKK826 pKa = 9.86 AEE828 pKa = 4.05 MNPVFVNRR836 pKa = 11.84 TPGQTTLGQPTTDD849 pKa = 3.28 TTGMTTADD857 pKa = 3.79 FLDD860 pKa = 3.58 II861 pKa = 4.88
Molecular weight: 91.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.268
IPC2_protein 5.27
IPC_protein 5.258
Toseland 5.283
ProMoST 5.423
Dawson 5.296
Bjellqvist 5.397
Wikipedia 5.181
Rodwell 5.219
Grimsley 5.245
Solomon 5.296
Lehninger 5.258
Nozaki 5.436
DTASelect 5.601
Thurlkill 5.308
EMBOSS 5.27
Sillero 5.512
Patrickios 4.317
IPC_peptide 5.296
IPC2_peptide 5.512
IPC2.peptide.svr19 5.471
Protein with the highest isoelectric point:
>tr|E3W913|E3W913_9VIRU Structural protein OS=Piscine myocarditis virus AL V-708 OX=912320 PE=4 SV=1
MM1 pKa = 6.82 ITKK4 pKa = 10.3 ALAQGRR10 pKa = 11.84 EE11 pKa = 4.04 EE12 pKa = 4.16 DD13 pKa = 3.76 WIKK16 pKa = 9.9 ITTNTTKK23 pKa = 10.35 IKK25 pKa = 10.47 LKK27 pKa = 9.7 SLRR30 pKa = 11.84 PLDD33 pKa = 3.81 GWEE36 pKa = 3.82 HH37 pKa = 6.1 SGWGLGLRR45 pKa = 11.84 LALEE49 pKa = 4.46 LVSEE53 pKa = 4.64 PTRR56 pKa = 11.84 EE57 pKa = 4.03 TMSGVIRR64 pKa = 11.84 QIRR67 pKa = 11.84 IGKK70 pKa = 9.4 LDD72 pKa = 3.39 PTKK75 pKa = 10.67 VFKK78 pKa = 10.93 SISNQAKK85 pKa = 9.57 VSGCGLCRR93 pKa = 11.84 EE94 pKa = 4.14 WKK96 pKa = 10.37 EE97 pKa = 3.92 YY98 pKa = 10.72 VNWEE102 pKa = 4.12 LLGGYY107 pKa = 8.03 QMITAQQMGEE117 pKa = 4.19 EE118 pKa = 4.48 VAHH121 pKa = 5.94 QVTEE125 pKa = 4.13 KK126 pKa = 10.87 AKK128 pKa = 10.59 SEE130 pKa = 4.04 EE131 pKa = 4.2 AGSDD135 pKa = 3.5 RR136 pKa = 11.84 INRR139 pKa = 11.84 ALEE142 pKa = 4.52 SILGPLRR149 pKa = 11.84 GTCTVPCSQRR159 pKa = 11.84 EE160 pKa = 4.0 FLLCRR165 pKa = 11.84 DD166 pKa = 3.28 KK167 pKa = 10.57 WAKK170 pKa = 8.19 NTSGYY175 pKa = 10.08 IGLGQNLGRR184 pKa = 11.84 QKK186 pKa = 10.52 IDD188 pKa = 2.96 VALNSDD194 pKa = 3.42 IKK196 pKa = 11.0 ALEE199 pKa = 3.74 RR200 pKa = 11.84 MMEE203 pKa = 4.12 GEE205 pKa = 4.08 FEE207 pKa = 4.0 NRR209 pKa = 11.84 PFIKK213 pKa = 10.28 SEE215 pKa = 4.03 PGKK218 pKa = 10.48 SRR220 pKa = 11.84 PVVNSNISCYY230 pKa = 10.92 LNLEE234 pKa = 4.42 YY235 pKa = 10.95 GWVTIKK241 pKa = 9.75 KK242 pKa = 7.39 TLKK245 pKa = 10.11 KK246 pKa = 10.41 YY247 pKa = 9.85 LGKK250 pKa = 9.22 RR251 pKa = 11.84 TTIFDD256 pKa = 3.91 DD257 pKa = 4.23 ASQKK261 pKa = 10.02 VLLWQEE267 pKa = 4.12 MIIGADD273 pKa = 3.15 NHH275 pKa = 6.87 RR276 pKa = 11.84 EE277 pKa = 3.84 IKK279 pKa = 10.89 VPLDD283 pKa = 3.14 YY284 pKa = 11.37 SRR286 pKa = 11.84 FDD288 pKa = 3.31 STIGKK293 pKa = 5.64 TQIMKK298 pKa = 10.17 CVRR301 pKa = 11.84 LLLDD305 pKa = 3.32 MVRR308 pKa = 11.84 GNDD311 pKa = 2.75 NWKK314 pKa = 10.27 RR315 pKa = 11.84 SVTEE319 pKa = 3.55 RR320 pKa = 11.84 FEE322 pKa = 4.12 RR323 pKa = 11.84 QTVYY327 pKa = 10.53 IDD329 pKa = 3.95 GYY331 pKa = 11.59 GKK333 pKa = 10.28 LRR335 pKa = 11.84 WEE337 pKa = 4.17 NSVLSGWRR345 pKa = 11.84 WTSIITSVINLAILKK360 pKa = 8.58 ATGADD365 pKa = 3.29 ASGVGIKK372 pKa = 10.26 VQGDD376 pKa = 3.55 DD377 pKa = 3.94 VKK379 pKa = 11.12 ISFQTIRR386 pKa = 11.84 EE387 pKa = 4.18 AEE389 pKa = 4.08 RR390 pKa = 11.84 CVEE393 pKa = 4.3 EE394 pKa = 4.55 INALGYY400 pKa = 9.78 EE401 pKa = 4.13 INPGKK406 pKa = 10.68 VFASRR411 pKa = 11.84 KK412 pKa = 7.51 RR413 pKa = 11.84 DD414 pKa = 3.1 EE415 pKa = 4.4 YY416 pKa = 11.26 LRR418 pKa = 11.84 MVAEE422 pKa = 4.38 EE423 pKa = 4.52 GKK425 pKa = 8.95 MAGYY429 pKa = 10.09 VIRR432 pKa = 11.84 CLPKK436 pKa = 9.92 IVFTSPTEE444 pKa = 4.08 EE445 pKa = 3.78 VTTWEE450 pKa = 3.67 EE451 pKa = 4.2 RR452 pKa = 11.84 IRR454 pKa = 11.84 GTVSKK459 pKa = 9.97 WMRR462 pKa = 11.84 VLSRR466 pKa = 11.84 GGDD469 pKa = 3.46 RR470 pKa = 11.84 EE471 pKa = 4.19 VAKK474 pKa = 10.98 YY475 pKa = 8.6 WMKK478 pKa = 10.88 RR479 pKa = 11.84 DD480 pKa = 3.45 LCGLSGEE487 pKa = 4.13 NSEE490 pKa = 6.53 LIDD493 pKa = 3.2 SWLRR497 pKa = 11.84 TPASVGGGGCAWLLGEE513 pKa = 4.51 GNLWTGLVLRR523 pKa = 11.84 EE524 pKa = 3.94 QEE526 pKa = 3.85 EE527 pKa = 4.64 AEE529 pKa = 4.13 VVEE532 pKa = 4.39 RR533 pKa = 11.84 SNHH536 pKa = 5.48 KK537 pKa = 10.09 MMSDD541 pKa = 3.57 YY542 pKa = 11.01 GGSVPARR549 pKa = 11.84 RR550 pKa = 11.84 WIKK553 pKa = 10.77 SVTKK557 pKa = 9.9 GAKK560 pKa = 8.87 PAFGFKK566 pKa = 10.01 RR567 pKa = 11.84 IEE569 pKa = 3.99 RR570 pKa = 11.84 VKK572 pKa = 10.88 PLGNWLKK579 pKa = 10.65 DD580 pKa = 3.44 LRR582 pKa = 11.84 PVAEE586 pKa = 4.48 SIKK589 pKa = 10.66 SISDD593 pKa = 3.57 LVEE596 pKa = 3.92 TSQVPKK602 pKa = 10.6 FRR604 pKa = 11.84 FKK606 pKa = 10.63 EE607 pKa = 4.04 IYY609 pKa = 9.65 DD610 pKa = 3.3 WTPFMVHH617 pKa = 5.93 KK618 pKa = 10.53 RR619 pKa = 11.84 NALIEE624 pKa = 4.2 EE625 pKa = 4.41 KK626 pKa = 10.52 NWDD629 pKa = 3.99 KK630 pKa = 11.42 LCSLYY635 pKa = 11.0 HH636 pKa = 6.07 NEE638 pKa = 4.5 GEE640 pKa = 4.04 VRR642 pKa = 11.84 LYY644 pKa = 11.03 KK645 pKa = 10.45 RR646 pKa = 11.84 ILPRR650 pKa = 11.84 WMWLSMIKK658 pKa = 10.37 NEE660 pKa = 4.16 LTWVSMPPNIANSEE674 pKa = 4.26 VASLAKK680 pKa = 10.24 EE681 pKa = 3.67 RR682 pKa = 11.84 VEE684 pKa = 3.94 YY685 pKa = 10.55 KK686 pKa = 10.66 VLSKK690 pKa = 10.37 VLRR693 pKa = 11.84 YY694 pKa = 9.44 HH695 pKa = 5.68 RR696 pKa = 11.84 TVSRR700 pKa = 11.84 EE701 pKa = 3.46 TLTRR705 pKa = 11.84 FSIGGEE711 pKa = 4.06 YY712 pKa = 10.43 VLSAKK717 pKa = 10.71 LNDD720 pKa = 3.73 RR721 pKa = 11.84 QRR723 pKa = 11.84 ILQQ726 pKa = 3.73
Molecular weight: 83.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.845
IPC2_protein 8.697
IPC_protein 8.58
Toseland 9.428
ProMoST 9.165
Dawson 9.677
Bjellqvist 9.414
Wikipedia 9.823
Rodwell 10.028
Grimsley 9.736
Solomon 9.706
Lehninger 9.677
Nozaki 9.604
DTASelect 9.355
Thurlkill 9.545
EMBOSS 9.867
Sillero 9.648
Patrickios 5.347
IPC_peptide 9.706
IPC2_peptide 8.009
IPC2.peptide.svr19 7.855
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1889
302
861
629.7
69.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.994 ± 2.506
1.535 ± 0.682
4.394 ± 0.369
7.253 ± 1.138
2.594 ± 0.243
10.323 ± 1.362
1.323 ± 0.225
4.87 ± 1.206
5.188 ± 2.078
7.517 ± 1.137
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.282 ± 0.537
4.553 ± 0.355
4.288 ± 0.813
3.123 ± 0.286
6.988 ± 0.607
5.664 ± 0.849
5.188 ± 0.479
8.947 ± 1.293
2.647 ± 0.523
2.329 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here