Piscine myocarditis virus AL V-708

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|E3W912|E3W912_9VIRU RNA-directed RNA polymerase OS=Piscine myocarditis virus AL V-708 OX=912320 PE=3 SV=1
MM1 pKa = 7.66EE2 pKa = 6.22PNTSVIATEE11 pKa = 4.01QQQAAMRR18 pKa = 11.84EE19 pKa = 4.41VEE21 pKa = 4.29AEE23 pKa = 3.5AAARR27 pKa = 11.84DD28 pKa = 3.8EE29 pKa = 4.39VVEE32 pKa = 4.8KK33 pKa = 10.67IAFAEE38 pKa = 4.48GAMMVQTRR46 pKa = 11.84RR47 pKa = 11.84LPSGKK52 pKa = 9.96SSVGGFLGEE61 pKa = 4.05LAQNIRR67 pKa = 11.84AMNRR71 pKa = 11.84SLHH74 pKa = 6.23TDD76 pKa = 2.82TNMLTEE82 pKa = 4.11GAMVDD87 pKa = 3.33RR88 pKa = 11.84ARR90 pKa = 11.84AKK92 pKa = 7.48VHH94 pKa = 6.15KK95 pKa = 9.94IIRR98 pKa = 11.84EE99 pKa = 4.08GNLDD103 pKa = 3.4SRR105 pKa = 11.84VFSNTGSNTMLSLWVPAVPGPPAVPEE131 pKa = 4.15HH132 pKa = 6.92WDD134 pKa = 3.43VAPSWFVCRR143 pKa = 11.84PGKK146 pKa = 10.37KK147 pKa = 10.03GGIKK151 pKa = 8.63ITQSASMAALNPLFRR166 pKa = 11.84GADD169 pKa = 3.29VGPIGTAVRR178 pKa = 11.84ADD180 pKa = 3.44VNAFSMNAVLGALRR194 pKa = 11.84AGGFNTEE201 pKa = 3.76HH202 pKa = 6.68SLVSFVEE209 pKa = 3.76PLIRR213 pKa = 11.84ILLMGVQTQDD223 pKa = 3.08RR224 pKa = 11.84GTSPWDD230 pKa = 3.15WVGGMSSRR238 pKa = 11.84IVNPLVFTTSGNFFPGGPNLRR259 pKa = 11.84VWGANDD265 pKa = 3.22TVARR269 pKa = 11.84IVNVEE274 pKa = 3.83DD275 pKa = 3.75YY276 pKa = 9.78MRR278 pKa = 11.84EE279 pKa = 3.75AAGEE283 pKa = 4.22GRR285 pKa = 11.84FDD287 pKa = 4.39AGWGPEE293 pKa = 4.04FWGGTGDD300 pKa = 3.79DD301 pKa = 3.85AVAVVPIRR309 pKa = 11.84AVEE312 pKa = 4.09AGLGEE317 pKa = 4.57VNAGWTLAHH326 pKa = 6.36MEE328 pKa = 4.33YY329 pKa = 9.87PVKK332 pKa = 10.77VRR334 pKa = 11.84LLDD337 pKa = 3.54VDD339 pKa = 4.46DD340 pKa = 4.01RR341 pKa = 11.84TIGPGGSLPLNANRR355 pKa = 11.84EE356 pKa = 4.11YY357 pKa = 9.54TAAGATHH364 pKa = 6.4VPGPYY369 pKa = 10.13ARR371 pKa = 11.84VLYY374 pKa = 10.5VVVDD378 pKa = 4.38QNADD382 pKa = 3.33RR383 pKa = 11.84CVGVRR388 pKa = 11.84VQGQGAVIDD397 pKa = 3.88VDD399 pKa = 3.56PALNYY404 pKa = 10.48VIGGADD410 pKa = 3.85LGMLPLIQWSVGLGAEE426 pKa = 4.59DD427 pKa = 3.66MAQGSIAQTQRR438 pKa = 11.84WVRR441 pKa = 11.84MYY443 pKa = 11.13GNEE446 pKa = 4.68DD447 pKa = 3.17DD448 pKa = 5.49WEE450 pKa = 4.69SAWHH454 pKa = 5.9LVSSAYY460 pKa = 8.9TVYY463 pKa = 10.78SPAFRR468 pKa = 11.84RR469 pKa = 11.84SGVAVEE475 pKa = 4.44GGFWAQPAAGAAPFPLGGLAGWVRR499 pKa = 11.84YY500 pKa = 9.8DD501 pKa = 3.47NQARR505 pKa = 11.84AAQVALCRR513 pKa = 11.84EE514 pKa = 4.13RR515 pKa = 11.84ADD517 pKa = 3.51MAEE520 pKa = 4.54CPWGGYY526 pKa = 7.07RR527 pKa = 11.84EE528 pKa = 4.04RR529 pKa = 11.84GVRR532 pKa = 11.84PGSVANWQYY541 pKa = 11.32VRR543 pKa = 11.84FDD545 pKa = 3.28PTVAVGVAAHH555 pKa = 6.47FWSVVKK561 pKa = 10.73VMVAPVPDD569 pKa = 3.78RR570 pKa = 11.84AAALADD576 pKa = 3.76MAWGKK581 pKa = 11.07GKK583 pKa = 9.66VQAMGEE589 pKa = 4.14DD590 pKa = 4.19VINGQMGQPEE600 pKa = 4.69SMMRR604 pKa = 11.84GVALNEE610 pKa = 4.07NQGLAAATVRR620 pKa = 11.84RR621 pKa = 11.84VVGLEE626 pKa = 3.87NEE628 pKa = 4.59SMQTTHH634 pKa = 6.25WSTTEE639 pKa = 3.66VAMNGYY645 pKa = 9.34YY646 pKa = 10.55GRR648 pKa = 11.84AGATAHH654 pKa = 6.63HH655 pKa = 6.67AAFPLSEE662 pKa = 4.39GGTMRR667 pKa = 11.84KK668 pKa = 9.79RR669 pKa = 11.84IPAIEE674 pKa = 3.84MRR676 pKa = 11.84EE677 pKa = 3.97NGVEE681 pKa = 4.0GDD683 pKa = 4.31LMNDD687 pKa = 3.43DD688 pKa = 5.37LYY690 pKa = 11.61SIGTAAGYY698 pKa = 9.76LAVEE702 pKa = 4.79GMAGAQGGIWDD713 pKa = 3.86VVQYY717 pKa = 9.15QLPGPDD723 pKa = 3.42DD724 pKa = 3.6EE725 pKa = 5.87ARR727 pKa = 11.84GVMNTVGAMGGWTRR741 pKa = 11.84AVTPVDD747 pKa = 3.4NVATMRR753 pKa = 11.84DD754 pKa = 3.33NGVEE758 pKa = 4.01GEE760 pKa = 4.12PCGIVMSLPTSGTAVVDD777 pKa = 4.04RR778 pKa = 11.84LANFGLPPARR788 pKa = 11.84AEE790 pKa = 3.92LRR792 pKa = 11.84EE793 pKa = 4.14VPFGGYY799 pKa = 7.64QRR801 pKa = 11.84SVTNTNHH808 pKa = 5.89RR809 pKa = 11.84VKK811 pKa = 10.85VSVSGGRR818 pKa = 11.84AVVQKK823 pKa = 10.92GNKK826 pKa = 9.86AEE828 pKa = 4.05MNPVFVNRR836 pKa = 11.84TPGQTTLGQPTTDD849 pKa = 3.28TTGMTTADD857 pKa = 3.79FLDD860 pKa = 3.58II861 pKa = 4.88

Molecular weight:
91.81 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|E3W913|E3W913_9VIRU Structural protein OS=Piscine myocarditis virus AL V-708 OX=912320 PE=4 SV=1
MM1 pKa = 6.82ITKK4 pKa = 10.3ALAQGRR10 pKa = 11.84EE11 pKa = 4.04EE12 pKa = 4.16DD13 pKa = 3.76WIKK16 pKa = 9.9ITTNTTKK23 pKa = 10.35IKK25 pKa = 10.47LKK27 pKa = 9.7SLRR30 pKa = 11.84PLDD33 pKa = 3.81GWEE36 pKa = 3.82HH37 pKa = 6.1SGWGLGLRR45 pKa = 11.84LALEE49 pKa = 4.46LVSEE53 pKa = 4.64PTRR56 pKa = 11.84EE57 pKa = 4.03TMSGVIRR64 pKa = 11.84QIRR67 pKa = 11.84IGKK70 pKa = 9.4LDD72 pKa = 3.39PTKK75 pKa = 10.67VFKK78 pKa = 10.93SISNQAKK85 pKa = 9.57VSGCGLCRR93 pKa = 11.84EE94 pKa = 4.14WKK96 pKa = 10.37EE97 pKa = 3.92YY98 pKa = 10.72VNWEE102 pKa = 4.12LLGGYY107 pKa = 8.03QMITAQQMGEE117 pKa = 4.19EE118 pKa = 4.48VAHH121 pKa = 5.94QVTEE125 pKa = 4.13KK126 pKa = 10.87AKK128 pKa = 10.59SEE130 pKa = 4.04EE131 pKa = 4.2AGSDD135 pKa = 3.5RR136 pKa = 11.84INRR139 pKa = 11.84ALEE142 pKa = 4.52SILGPLRR149 pKa = 11.84GTCTVPCSQRR159 pKa = 11.84EE160 pKa = 4.0FLLCRR165 pKa = 11.84DD166 pKa = 3.28KK167 pKa = 10.57WAKK170 pKa = 8.19NTSGYY175 pKa = 10.08IGLGQNLGRR184 pKa = 11.84QKK186 pKa = 10.52IDD188 pKa = 2.96VALNSDD194 pKa = 3.42IKK196 pKa = 11.0ALEE199 pKa = 3.74RR200 pKa = 11.84MMEE203 pKa = 4.12GEE205 pKa = 4.08FEE207 pKa = 4.0NRR209 pKa = 11.84PFIKK213 pKa = 10.28SEE215 pKa = 4.03PGKK218 pKa = 10.48SRR220 pKa = 11.84PVVNSNISCYY230 pKa = 10.92LNLEE234 pKa = 4.42YY235 pKa = 10.95GWVTIKK241 pKa = 9.75KK242 pKa = 7.39TLKK245 pKa = 10.11KK246 pKa = 10.41YY247 pKa = 9.85LGKK250 pKa = 9.22RR251 pKa = 11.84TTIFDD256 pKa = 3.91DD257 pKa = 4.23ASQKK261 pKa = 10.02VLLWQEE267 pKa = 4.12MIIGADD273 pKa = 3.15NHH275 pKa = 6.87RR276 pKa = 11.84EE277 pKa = 3.84IKK279 pKa = 10.89VPLDD283 pKa = 3.14YY284 pKa = 11.37SRR286 pKa = 11.84FDD288 pKa = 3.31STIGKK293 pKa = 5.64TQIMKK298 pKa = 10.17CVRR301 pKa = 11.84LLLDD305 pKa = 3.32MVRR308 pKa = 11.84GNDD311 pKa = 2.75NWKK314 pKa = 10.27RR315 pKa = 11.84SVTEE319 pKa = 3.55RR320 pKa = 11.84FEE322 pKa = 4.12RR323 pKa = 11.84QTVYY327 pKa = 10.53IDD329 pKa = 3.95GYY331 pKa = 11.59GKK333 pKa = 10.28LRR335 pKa = 11.84WEE337 pKa = 4.17NSVLSGWRR345 pKa = 11.84WTSIITSVINLAILKK360 pKa = 8.58ATGADD365 pKa = 3.29ASGVGIKK372 pKa = 10.26VQGDD376 pKa = 3.55DD377 pKa = 3.94VKK379 pKa = 11.12ISFQTIRR386 pKa = 11.84EE387 pKa = 4.18AEE389 pKa = 4.08RR390 pKa = 11.84CVEE393 pKa = 4.3EE394 pKa = 4.55INALGYY400 pKa = 9.78EE401 pKa = 4.13INPGKK406 pKa = 10.68VFASRR411 pKa = 11.84KK412 pKa = 7.51RR413 pKa = 11.84DD414 pKa = 3.1EE415 pKa = 4.4YY416 pKa = 11.26LRR418 pKa = 11.84MVAEE422 pKa = 4.38EE423 pKa = 4.52GKK425 pKa = 8.95MAGYY429 pKa = 10.09VIRR432 pKa = 11.84CLPKK436 pKa = 9.92IVFTSPTEE444 pKa = 4.08EE445 pKa = 3.78VTTWEE450 pKa = 3.67EE451 pKa = 4.2RR452 pKa = 11.84IRR454 pKa = 11.84GTVSKK459 pKa = 9.97WMRR462 pKa = 11.84VLSRR466 pKa = 11.84GGDD469 pKa = 3.46RR470 pKa = 11.84EE471 pKa = 4.19VAKK474 pKa = 10.98YY475 pKa = 8.6WMKK478 pKa = 10.88RR479 pKa = 11.84DD480 pKa = 3.45LCGLSGEE487 pKa = 4.13NSEE490 pKa = 6.53LIDD493 pKa = 3.2SWLRR497 pKa = 11.84TPASVGGGGCAWLLGEE513 pKa = 4.51GNLWTGLVLRR523 pKa = 11.84EE524 pKa = 3.94QEE526 pKa = 3.85EE527 pKa = 4.64AEE529 pKa = 4.13VVEE532 pKa = 4.39RR533 pKa = 11.84SNHH536 pKa = 5.48KK537 pKa = 10.09MMSDD541 pKa = 3.57YY542 pKa = 11.01GGSVPARR549 pKa = 11.84RR550 pKa = 11.84WIKK553 pKa = 10.77SVTKK557 pKa = 9.9GAKK560 pKa = 8.87PAFGFKK566 pKa = 10.01RR567 pKa = 11.84IEE569 pKa = 3.99RR570 pKa = 11.84VKK572 pKa = 10.88PLGNWLKK579 pKa = 10.65DD580 pKa = 3.44LRR582 pKa = 11.84PVAEE586 pKa = 4.48SIKK589 pKa = 10.66SISDD593 pKa = 3.57LVEE596 pKa = 3.92TSQVPKK602 pKa = 10.6FRR604 pKa = 11.84FKK606 pKa = 10.63EE607 pKa = 4.04IYY609 pKa = 9.65DD610 pKa = 3.3WTPFMVHH617 pKa = 5.93KK618 pKa = 10.53RR619 pKa = 11.84NALIEE624 pKa = 4.2EE625 pKa = 4.41KK626 pKa = 10.52NWDD629 pKa = 3.99KK630 pKa = 11.42LCSLYY635 pKa = 11.0HH636 pKa = 6.07NEE638 pKa = 4.5GEE640 pKa = 4.04VRR642 pKa = 11.84LYY644 pKa = 11.03KK645 pKa = 10.45RR646 pKa = 11.84ILPRR650 pKa = 11.84WMWLSMIKK658 pKa = 10.37NEE660 pKa = 4.16LTWVSMPPNIANSEE674 pKa = 4.26VASLAKK680 pKa = 10.24EE681 pKa = 3.67RR682 pKa = 11.84VEE684 pKa = 3.94YY685 pKa = 10.55KK686 pKa = 10.66VLSKK690 pKa = 10.37VLRR693 pKa = 11.84YY694 pKa = 9.44HH695 pKa = 5.68RR696 pKa = 11.84TVSRR700 pKa = 11.84EE701 pKa = 3.46TLTRR705 pKa = 11.84FSIGGEE711 pKa = 4.06YY712 pKa = 10.43VLSAKK717 pKa = 10.71LNDD720 pKa = 3.73RR721 pKa = 11.84QRR723 pKa = 11.84ILQQ726 pKa = 3.73

Molecular weight:
83.06 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1889

302

861

629.7

69.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.994 ± 2.506

1.535 ± 0.682

4.394 ± 0.369

7.253 ± 1.138

2.594 ± 0.243

10.323 ± 1.362

1.323 ± 0.225

4.87 ± 1.206

5.188 ± 2.078

7.517 ± 1.137

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.282 ± 0.537

4.553 ± 0.355

4.288 ± 0.813

3.123 ± 0.286

6.988 ± 0.607

5.664 ± 0.849

5.188 ± 0.479

8.947 ± 1.293

2.647 ± 0.523

2.329 ± 0.234

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski