Streptococcus phage Javan240

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D6AYF7|A0A4D6AYF7_9CAUD HIRAN domain-containing protein OS=Streptococcus phage Javan240 OX=2548064 GN=Javan240_0002 PE=4 SV=1
MM1 pKa = 8.0DD2 pKa = 4.56KK3 pKa = 11.2VKK5 pKa = 10.77LFQNEE10 pKa = 4.09VLGSGFDD17 pKa = 3.23IDD19 pKa = 6.24GYY21 pKa = 10.9FGWQCWDD28 pKa = 3.58GYY30 pKa = 11.61AKK32 pKa = 10.42YY33 pKa = 10.01CLWLGVPFSNCTDD46 pKa = 2.95SYY48 pKa = 10.4YY49 pKa = 11.44VKK51 pKa = 10.64DD52 pKa = 3.58LWNQRR57 pKa = 11.84HH58 pKa = 5.51EE59 pKa = 4.37NGILDD64 pKa = 3.9YY65 pKa = 10.74FDD67 pKa = 4.28EE68 pKa = 4.99VEE70 pKa = 4.19DD71 pKa = 4.22LEE73 pKa = 4.98GGEE76 pKa = 3.97VCIFIEE82 pKa = 4.73SSLTPVSHH90 pKa = 5.71VAIFIGDD97 pKa = 3.36VDD99 pKa = 4.02GVNGQFLGQNQGGEE113 pKa = 4.09AGPFGGGAFDD123 pKa = 3.59IQIFPYY129 pKa = 10.32SILYY133 pKa = 6.8PTAFRR138 pKa = 11.84PKK140 pKa = 10.53GEE142 pKa = 4.23SLPKK146 pKa = 10.03QEE148 pKa = 4.78LKK150 pKa = 10.9EE151 pKa = 3.99AVTEE155 pKa = 4.19VMEE158 pKa = 4.26SHH160 pKa = 6.89EE161 pKa = 4.3APFYY165 pKa = 10.91PEE167 pKa = 4.96DD168 pKa = 3.44ATFTVGDD175 pKa = 3.59SPINVRR181 pKa = 11.84RR182 pKa = 11.84EE183 pKa = 3.75PNLNGEE189 pKa = 4.31IVAVYY194 pKa = 9.94QPGDD198 pKa = 3.44KK199 pKa = 10.36VHH201 pKa = 6.55YY202 pKa = 9.59DD203 pKa = 3.47SKK205 pKa = 11.33GSNDD209 pKa = 3.13GYY211 pKa = 11.06RR212 pKa = 11.84WISYY216 pKa = 9.87VGEE219 pKa = 4.28SGNRR223 pKa = 11.84NYY225 pKa = 10.82LAIGQTDD232 pKa = 3.5EE233 pKa = 4.12AGNRR237 pKa = 11.84IDD239 pKa = 4.83LWGEE243 pKa = 3.77LSS245 pKa = 3.33

Molecular weight:
27.34 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D6AW59|A0A4D6AW59_9CAUD Uncharacterized protein OS=Streptococcus phage Javan240 OX=2548064 GN=Javan240_0049 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.11SGPMQKK8 pKa = 9.81VLSQYY13 pKa = 11.37ASDD16 pKa = 3.52IQDD19 pKa = 3.21RR20 pKa = 11.84CGDD23 pKa = 3.88GYY25 pKa = 11.71VKK27 pKa = 10.44DD28 pKa = 3.23IHH30 pKa = 7.24VGKK33 pKa = 10.1NRR35 pKa = 11.84ANASVSAKK43 pKa = 7.77TRR45 pKa = 11.84KK46 pKa = 9.43AKK48 pKa = 10.23KK49 pKa = 10.29DD50 pKa = 3.33NLKK53 pKa = 11.11NNTLLKK59 pKa = 10.42AVRR62 pKa = 3.79

Molecular weight:
6.85 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

9907

40

1292

194.3

21.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.823 ± 0.623

0.696 ± 0.13

6.036 ± 0.282

7.5 ± 0.5

4.441 ± 0.251

7.086 ± 0.677

1.544 ± 0.171

7.601 ± 0.317

8.338 ± 0.396

8.136 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.11 ± 0.177

5.703 ± 0.206

2.635 ± 0.236

3.957 ± 0.19

4.209 ± 0.421

6.319 ± 0.317

5.683 ± 0.335

6.137 ± 0.256

1.342 ± 0.217

3.704 ± 0.273

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski