Streptococcus phage Javan240
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AYF7|A0A4D6AYF7_9CAUD HIRAN domain-containing protein OS=Streptococcus phage Javan240 OX=2548064 GN=Javan240_0002 PE=4 SV=1
MM1 pKa = 8.0 DD2 pKa = 4.56 KK3 pKa = 11.2 VKK5 pKa = 10.77 LFQNEE10 pKa = 4.09 VLGSGFDD17 pKa = 3.23 IDD19 pKa = 6.24 GYY21 pKa = 10.9 FGWQCWDD28 pKa = 3.58 GYY30 pKa = 11.61 AKK32 pKa = 10.42 YY33 pKa = 10.01 CLWLGVPFSNCTDD46 pKa = 2.95 SYY48 pKa = 10.4 YY49 pKa = 11.44 VKK51 pKa = 10.64 DD52 pKa = 3.58 LWNQRR57 pKa = 11.84 HH58 pKa = 5.51 EE59 pKa = 4.37 NGILDD64 pKa = 3.9 YY65 pKa = 10.74 FDD67 pKa = 4.28 EE68 pKa = 4.99 VEE70 pKa = 4.19 DD71 pKa = 4.22 LEE73 pKa = 4.98 GGEE76 pKa = 3.97 VCIFIEE82 pKa = 4.73 SSLTPVSHH90 pKa = 5.71 VAIFIGDD97 pKa = 3.36 VDD99 pKa = 4.02 GVNGQFLGQNQGGEE113 pKa = 4.09 AGPFGGGAFDD123 pKa = 3.59 IQIFPYY129 pKa = 10.32 SILYY133 pKa = 6.8 PTAFRR138 pKa = 11.84 PKK140 pKa = 10.53 GEE142 pKa = 4.23 SLPKK146 pKa = 10.03 QEE148 pKa = 4.78 LKK150 pKa = 10.9 EE151 pKa = 3.99 AVTEE155 pKa = 4.19 VMEE158 pKa = 4.26 SHH160 pKa = 6.89 EE161 pKa = 4.3 APFYY165 pKa = 10.91 PEE167 pKa = 4.96 DD168 pKa = 3.44 ATFTVGDD175 pKa = 3.59 SPINVRR181 pKa = 11.84 RR182 pKa = 11.84 EE183 pKa = 3.75 PNLNGEE189 pKa = 4.31 IVAVYY194 pKa = 9.94 QPGDD198 pKa = 3.44 KK199 pKa = 10.36 VHH201 pKa = 6.55 YY202 pKa = 9.59 DD203 pKa = 3.47 SKK205 pKa = 11.33 GSNDD209 pKa = 3.13 GYY211 pKa = 11.06 RR212 pKa = 11.84 WISYY216 pKa = 9.87 VGEE219 pKa = 4.28 SGNRR223 pKa = 11.84 NYY225 pKa = 10.82 LAIGQTDD232 pKa = 3.5 EE233 pKa = 4.12 AGNRR237 pKa = 11.84 IDD239 pKa = 4.83 LWGEE243 pKa = 3.77 LSS245 pKa = 3.33
Molecular weight: 27.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.927
IPC2_protein 4.215
IPC_protein 4.164
Toseland 3.986
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.024
Rodwell 3.999
Grimsley 3.897
Solomon 4.126
Lehninger 4.088
Nozaki 4.24
DTASelect 4.418
Thurlkill 4.012
EMBOSS 4.037
Sillero 4.279
Patrickios 1.303
IPC_peptide 4.126
IPC2_peptide 4.266
IPC2.peptide.svr19 4.164
Protein with the highest isoelectric point:
>tr|A0A4D6AW59|A0A4D6AW59_9CAUD Uncharacterized protein OS=Streptococcus phage Javan240 OX=2548064 GN=Javan240_0049 PE=4 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.11 SGPMQKK8 pKa = 9.81 VLSQYY13 pKa = 11.37 ASDD16 pKa = 3.52 IQDD19 pKa = 3.21 RR20 pKa = 11.84 CGDD23 pKa = 3.88 GYY25 pKa = 11.71 VKK27 pKa = 10.44 DD28 pKa = 3.23 IHH30 pKa = 7.24 VGKK33 pKa = 10.1 NRR35 pKa = 11.84 ANASVSAKK43 pKa = 7.77 TRR45 pKa = 11.84 KK46 pKa = 9.43 AKK48 pKa = 10.23 KK49 pKa = 10.29 DD50 pKa = 3.33 NLKK53 pKa = 11.11 NNTLLKK59 pKa = 10.42 AVRR62 pKa = 3.79
Molecular weight: 6.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.138
IPC2_protein 9.487
IPC_protein 9.472
Toseland 10.482
ProMoST 10.101
Dawson 10.57
Bjellqvist 10.145
Wikipedia 10.672
Rodwell 11.257
Grimsley 10.599
Solomon 10.599
Lehninger 10.584
Nozaki 10.438
DTASelect 10.145
Thurlkill 10.452
EMBOSS 10.847
Sillero 10.467
Patrickios 11.023
IPC_peptide 10.599
IPC2_peptide 8.653
IPC2.peptide.svr19 8.481
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
9907
40
1292
194.3
21.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.823 ± 0.623
0.696 ± 0.13
6.036 ± 0.282
7.5 ± 0.5
4.441 ± 0.251
7.086 ± 0.677
1.544 ± 0.171
7.601 ± 0.317
8.338 ± 0.396
8.136 ± 0.231
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.11 ± 0.177
5.703 ± 0.206
2.635 ± 0.236
3.957 ± 0.19
4.209 ± 0.421
6.319 ± 0.317
5.683 ± 0.335
6.137 ± 0.256
1.342 ± 0.217
3.704 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here