Vibrio phage ND1-fs1

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Fibrovirus; unclassified Fibrovirus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1CJD2|E1CJD2_9VIRU DNA primase OS=Vibrio phage ND1-fs1 OX=867695 PE=4 SV=1
MM1 pKa = 6.37VTNGLTDD8 pKa = 3.62CDD10 pKa = 3.7RR11 pKa = 11.84VCFFAEE17 pKa = 4.36FAMITALTGWGDD29 pKa = 3.56HH30 pKa = 5.71QGEE33 pKa = 4.44EE34 pKa = 4.62LRR36 pKa = 11.84SSAQGGQITIYY47 pKa = 10.72GDD49 pKa = 3.33SSPNFWGLL57 pKa = 3.09

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1CJD3|E1CJD3_9VIRU Uncharacterized protein OS=Vibrio phage ND1-fs1 OX=867695 PE=4 SV=1
MM1 pKa = 7.51ANSAKK6 pKa = 10.25KK7 pKa = 8.22QTLSQSVNPFVTIQLTSGALPRR29 pKa = 11.84FLAYY33 pKa = 10.25GGFNSDD39 pKa = 2.52GSQRR43 pKa = 11.84FSVTSVSTDD52 pKa = 2.81AHH54 pKa = 6.7KK55 pKa = 10.72MPLPTANAILPKK67 pKa = 10.13IKK69 pKa = 10.48RR70 pKa = 11.84NWPLAQISYY79 pKa = 8.36QVKK82 pKa = 9.77

Molecular weight: 8.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

2117

52

493

176.4

19.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.991 ± 0.706

1.889 ± 0.282

5.716 ± 0.709

5.102 ± 0.482

5.102 ± 0.203

6.094 ± 0.394

1.889 ± 0.546

7.463 ± 0.794

6.141 ± 0.592

8.219 ± 1.002

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.929 ± 0.476

4.629 ± 0.653

5.432 ± 0.742

4.015 ± 0.343

4.487 ± 0.818

6.944 ± 0.93

5.527 ± 0.692

6.141 ± 0.402

1.417 ± 0.316

3.873 ± 0.588
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski