Bacillus phage phi105 (Bacteriophage phi-105)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Spizizenvirus; Bacillus virus phi105

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9ZXE1|Q9ZXE1_BPPH1 ORF42 OS=Bacillus phage phi105 OX=10717 PE=4 SV=1
MM1 pKa = 6.7IQVYY5 pKa = 9.81EE6 pKa = 3.99YY7 pKa = 11.0DD8 pKa = 3.64EE9 pKa = 4.93NFILTKK15 pKa = 9.67PVPIEE20 pKa = 3.83PDD22 pKa = 2.93EE23 pKa = 4.5EE24 pKa = 4.44GNYY27 pKa = 8.66TIPDD31 pKa = 3.77NCTIIQPPSFIKK43 pKa = 10.66AMYY46 pKa = 9.83HH47 pKa = 5.26PAEE50 pKa = 4.8KK51 pKa = 9.59IWTEE55 pKa = 3.5AATEE59 pKa = 4.09EE60 pKa = 4.38EE61 pKa = 4.6KK62 pKa = 10.98KK63 pKa = 10.93SLEE66 pKa = 3.96KK67 pKa = 10.56QIEE70 pKa = 4.43SGRR73 pKa = 11.84VPSPVDD79 pKa = 3.13EE80 pKa = 5.44LKK82 pKa = 10.95AQNAAVTTQLAEE94 pKa = 4.16AQSLAEE100 pKa = 4.45SQAQMIANLYY110 pKa = 10.67LMLAEE115 pKa = 4.73GGKK118 pKa = 10.49GVV120 pKa = 3.49

Molecular weight: 13.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9ZXG0|Q9ZXG0_BPPH1 ORF23 OS=Bacillus phage phi105 OX=10717 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.12KK3 pKa = 10.05ISVFFAALFNPRR15 pKa = 11.84VFKK18 pKa = 10.9KK19 pKa = 10.58GFSFFLLILNDD30 pKa = 4.22LLFMAGAAFILTAVYY45 pKa = 9.8RR46 pKa = 11.84WSTNIGLILTGVFLMFYY63 pKa = 10.32AYY65 pKa = 10.55LISKK69 pKa = 9.59KK70 pKa = 10.54ARR72 pKa = 3.38

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11745

56

1018

230.3

25.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.612 ± 0.735

0.69 ± 0.112

5.986 ± 0.226

8.054 ± 0.387

3.883 ± 0.224

6.794 ± 0.463

1.848 ± 0.21

6.232 ± 0.263

8.974 ± 0.3

7.867 ± 0.318

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.648 ± 0.181

4.981 ± 0.196

3.559 ± 0.338

4.129 ± 0.349

4.436 ± 0.269

6.232 ± 0.257

5.747 ± 0.251

5.407 ± 0.261

1.064 ± 0.126

3.857 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski