Firmicutes bacterium CAG:466

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2676 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6PXC2|R6PXC2_9FIRM ANTAR domain-containing protein OS=Firmicutes bacterium CAG:466 OX=1263025 GN=BN668_01785 PE=4 SV=1
MM1 pKa = 7.36HH2 pKa = 7.48CPYY5 pKa = 9.75CKK7 pKa = 10.3HH8 pKa = 6.49EE9 pKa = 4.13ISEE12 pKa = 4.13NANFCEE18 pKa = 4.14QCGKK22 pKa = 10.6KK23 pKa = 9.58IPKK26 pKa = 10.01CPTCGHH32 pKa = 6.94IIWKK36 pKa = 9.88KK37 pKa = 10.61EE38 pKa = 3.86NFCPMDD44 pKa = 3.86GTPLPSEE51 pKa = 5.15FFADD55 pKa = 3.48WPLEE59 pKa = 4.0EE60 pKa = 4.54VSLSEE65 pKa = 5.23EE66 pKa = 3.57ITAPEE71 pKa = 4.71DD72 pKa = 3.54PQQEE76 pKa = 4.18SSYY79 pKa = 11.43SEE81 pKa = 3.65MDD83 pKa = 3.31APEE86 pKa = 4.67SDD88 pKa = 3.14ITDD91 pKa = 3.46EE92 pKa = 5.1DD93 pKa = 5.55DD94 pKa = 3.53YY95 pKa = 12.13DD96 pKa = 4.07EE97 pKa = 6.46DD98 pKa = 4.4FDD100 pKa = 5.51EE101 pKa = 6.64DD102 pKa = 3.83FDD104 pKa = 5.1EE105 pKa = 6.66DD106 pKa = 4.23FDD108 pKa = 5.13EE109 pKa = 6.65DD110 pKa = 3.81FDD112 pKa = 5.82KK113 pKa = 11.52DD114 pKa = 3.55FDD116 pKa = 4.3EE117 pKa = 6.36DD118 pKa = 4.42FDD120 pKa = 5.27DD121 pKa = 5.95FEE123 pKa = 7.46DD124 pKa = 4.31YY125 pKa = 11.13DD126 pKa = 3.95EE127 pKa = 5.39SEE129 pKa = 4.42EE130 pKa = 4.78SEE132 pKa = 4.1KK133 pKa = 10.83TNWKK137 pKa = 9.56PILTAAAIIGVICVFASYY155 pKa = 10.34IAIVTGPGTLKK166 pKa = 10.23KK167 pKa = 10.33DD168 pKa = 3.55QNIHH172 pKa = 5.85VEE174 pKa = 4.2ASAKK178 pKa = 10.16QSASQEE184 pKa = 3.52ADD186 pKa = 2.73ADD188 pKa = 4.29VIFQHH193 pKa = 6.73SNYY196 pKa = 9.73TLLLPQNDD204 pKa = 3.56TMLL207 pKa = 4.64

Molecular weight: 23.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6R0U7|R6R0U7_9FIRM GntR family transcriptional regulator OS=Firmicutes bacterium CAG:466 OX=1263025 GN=BN668_00383 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.73KK9 pKa = 7.52RR10 pKa = 11.84QRR12 pKa = 11.84SRR14 pKa = 11.84EE15 pKa = 3.65HH16 pKa = 6.35GFRR19 pKa = 11.84KK20 pKa = 9.96RR21 pKa = 11.84MRR23 pKa = 11.84TANGRR28 pKa = 11.84KK29 pKa = 8.83VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2676

0

2676

766065

29

2016

286.3

31.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.255 ± 0.049

1.525 ± 0.026

5.449 ± 0.042

7.814 ± 0.055

4.256 ± 0.039

7.303 ± 0.044

1.79 ± 0.022

6.788 ± 0.045

6.542 ± 0.043

9.149 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.279 ± 0.025

3.923 ± 0.038

3.452 ± 0.027

3.464 ± 0.033

4.238 ± 0.035

5.214 ± 0.035

5.639 ± 0.041

7.195 ± 0.037

0.938 ± 0.019

3.786 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski