Amino acid dipepetide frequency for Firmicutes bacterium CAG:466

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.661AlaAla: 8.661 ± 0.167	1.167AlaCys: 1.167 ± 0.039	4.788AlaAsp: 4.788 ± 0.082	7.247AlaGlu: 7.247 ± 0.116	3.492AlaPhe: 3.492 ± 0.067	6.083AlaGly: 6.083 ± 0.102	1.13AlaHis: 1.13 ± 0.039	5.09AlaIle: 5.09 ± 0.078	5.715AlaLys: 5.715 ± 0.085	7.745AlaLeu: 7.745 ± 0.121	2.971AlaMet: 2.971 ± 0.061	2.676AlaAsn: 2.676 ± 0.064	2.374AlaPro: 2.374 ± 0.056	2.651AlaGln: 2.651 ± 0.074	2.71AlaArg: 2.71 ± 0.06	3.637AlaSer: 3.637 ± 0.071	3.544AlaThr: 3.544 ± 0.071	7.095AlaVal: 7.095 ± 0.125	0.662AlaTrp: 0.662 ± 0.03	3.105AlaTyr: 3.105 ± 0.07	0.004AlaXaa: 0.004 ± 0.002
Cys	1.119CysAla: 1.119 ± 0.047	0.351CysCys: 0.351 ± 0.027	0.86CysAsp: 0.86 ± 0.034	0.892CysGlu: 0.892 ± 0.039	0.753CysPhe: 0.753 ± 0.05	1.722CysGly: 1.722 ± 0.059	0.368CysHis: 0.368 ± 0.027	1.05CysIle: 1.05 ± 0.038	0.762CysLys: 0.762 ± 0.028	1.202CysLeu: 1.202 ± 0.041	0.469CysMet: 0.469 ± 0.024	0.531CysAsn: 0.531 ± 0.031	0.661CysPro: 0.661 ± 0.037	0.487CysGln: 0.487 ± 0.026	0.724CysArg: 0.724 ± 0.03	0.786CysSer: 0.786 ± 0.032	0.749CysThr: 0.749 ± 0.029	1.072CysVal: 1.072 ± 0.041	0.151CysTrp: 0.151 ± 0.013	0.544CysTyr: 0.544 ± 0.031	0.0CysXaa: 0.0 ± 0.0
Asp	4.488AspAla: 4.488 ± 0.084	0.856AspCys: 0.856 ± 0.034	2.705AspAsp: 2.705 ± 0.072	4.087AspGlu: 4.087 ± 0.077	2.915AspPhe: 2.915 ± 0.071	4.571AspGly: 4.571 ± 0.081	0.953AspHis: 0.953 ± 0.035	4.007AspIle: 4.007 ± 0.076	3.192AspLys: 3.192 ± 0.066	4.791AspLeu: 4.791 ± 0.078	1.972AspMet: 1.972 ± 0.054	1.923AspAsn: 1.923 ± 0.06	1.7AspPro: 1.7 ± 0.054	1.335AspGln: 1.335 ± 0.045	2.123AspArg: 2.123 ± 0.052	2.566AspSer: 2.566 ± 0.062	3.169AspThr: 3.169 ± 0.061	4.162AspVal: 4.162 ± 0.076	0.623AspTrp: 0.623 ± 0.029	2.352AspTyr: 2.352 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	6.238GluAla: 6.238 ± 0.103	0.693GluCys: 0.693 ± 0.032	4.045GluAsp: 4.045 ± 0.077	7.64GluGlu: 7.64 ± 0.148	2.189GluPhe: 2.189 ± 0.053	4.938GluGly: 4.938 ± 0.078	1.224GluHis: 1.224 ± 0.043	5.754GluIle: 5.754 ± 0.093	7.347GluLys: 7.347 ± 0.119	6.383GluLeu: 6.383 ± 0.095	2.872GluMet: 2.872 ± 0.059	4.365GluAsn: 4.365 ± 0.084	1.992GluPro: 1.992 ± 0.065	3.098GluGln: 3.098 ± 0.072	3.53GluArg: 3.53 ± 0.079	3.28GluSer: 3.28 ± 0.068	4.368GluThr: 4.368 ± 0.085	4.925GluVal: 4.925 ± 0.091	0.62GluTrp: 0.62 ± 0.029	2.634GluTyr: 2.634 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	3.72PheAla: 3.72 ± 0.08	0.942PheCys: 0.942 ± 0.044	2.475PheAsp: 2.475 ± 0.054	2.284PheGlu: 2.284 ± 0.056	2.158PhePhe: 2.158 ± 0.063	3.429PheGly: 3.429 ± 0.08	0.956PheHis: 0.956 ± 0.037	2.448PheIle: 2.448 ± 0.069	1.457PheLys: 1.457 ± 0.049	4.513PheLeu: 4.513 ± 0.094	1.191PheMet: 1.191 ± 0.043	1.252PheAsn: 1.252 ± 0.042	1.629PhePro: 1.629 ± 0.052	1.602PheGln: 1.602 ± 0.047	1.88PheArg: 1.88 ± 0.047	2.881PheSer: 2.881 ± 0.07	2.407PheThr: 2.407 ± 0.053	3.028PheVal: 3.028 ± 0.075	0.518PheTrp: 0.518 ± 0.031	1.784PheTyr: 1.784 ± 0.053	0.001PheXaa: 0.001 ± 0.001
Gly	5.49GlyAla: 5.49 ± 0.092	1.347GlyCys: 1.347 ± 0.048	3.613GlyAsp: 3.613 ± 0.075	5.202GlyGlu: 5.202 ± 0.094	3.27GlyPhe: 3.27 ± 0.063	5.382GlyGly: 5.382 ± 0.116	1.265GlyHis: 1.265 ± 0.041	5.955GlyIle: 5.955 ± 0.1	5.68GlyLys: 5.68 ± 0.088	6.037GlyLeu: 6.037 ± 0.103	2.549GlyMet: 2.549 ± 0.056	2.987GlyAsn: 2.987 ± 0.065	1.282GlyPro: 1.282 ± 0.045	2.16GlyGln: 2.16 ± 0.06	3.091GlyArg: 3.091 ± 0.06	3.971GlySer: 3.971 ± 0.073	4.566GlyThr: 4.566 ± 0.09	5.493GlyVal: 5.493 ± 0.096	0.705GlyTrp: 0.705 ± 0.029	2.987GlyTyr: 2.987 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.171HisAla: 1.171 ± 0.046	0.356HisCys: 0.356 ± 0.023	0.865HisAsp: 0.865 ± 0.038	1.008HisGlu: 1.008 ± 0.038	0.873HisPhe: 0.873 ± 0.031	1.281HisGly: 1.281 ± 0.038	0.523HisHis: 0.523 ± 0.032	1.427HisIle: 1.427 ± 0.042	0.958HisLys: 0.958 ± 0.039	1.73HisLeu: 1.73 ± 0.051	0.573HisMet: 0.573 ± 0.027	0.689HisAsn: 0.689 ± 0.035	0.868HisPro: 0.868 ± 0.035	0.58HisGln: 0.58 ± 0.026	0.839HisArg: 0.839 ± 0.036	0.996HisSer: 0.996 ± 0.038	1.081HisThr: 1.081 ± 0.041	1.245HisVal: 1.245 ± 0.042	0.187HisTrp: 0.187 ± 0.014	0.647HisTyr: 0.647 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.806IleAla: 5.806 ± 0.093	1.283IleCys: 1.283 ± 0.043	3.736IleAsp: 3.736 ± 0.077	3.805IleGlu: 3.805 ± 0.077	3.049IlePhe: 3.049 ± 0.072	4.959IleGly: 4.959 ± 0.101	1.334IleHis: 1.334 ± 0.043	4.441IleIle: 4.441 ± 0.083	3.215IleLys: 3.215 ± 0.077	7.011IleLeu: 7.011 ± 0.109	1.869IleMet: 1.869 ± 0.049	2.462IleAsn: 2.462 ± 0.058	3.069IlePro: 3.069 ± 0.06	2.365IleGln: 2.365 ± 0.063	3.193IleArg: 3.193 ± 0.068	4.574IleSer: 4.574 ± 0.081	3.984IleThr: 3.984 ± 0.076	4.63IleVal: 4.63 ± 0.086	0.599IleTrp: 0.599 ± 0.031	2.489IleTyr: 2.489 ± 0.068	0.0IleXaa: 0.0 ± 0.0
Lys	5.176LysAla: 5.176 ± 0.089	0.584LysCys: 0.584 ± 0.031	3.655LysAsp: 3.655 ± 0.075	6.566LysGlu: 6.566 ± 0.091	1.818LysPhe: 1.818 ± 0.051	4.565LysGly: 4.565 ± 0.076	1.018LysHis: 1.018 ± 0.037	4.537LysIle: 4.537 ± 0.08	5.485LysLys: 5.485 ± 0.101	5.135LysLeu: 5.135 ± 0.087	2.308LysMet: 2.308 ± 0.056	3.253LysAsn: 3.253 ± 0.08	1.817LysPro: 1.817 ± 0.055	2.48LysGln: 2.48 ± 0.059	3.004LysArg: 3.004 ± 0.068	2.901LysSer: 2.901 ± 0.066	3.846LysThr: 3.846 ± 0.083	4.223LysVal: 4.223 ± 0.077	0.564LysTrp: 0.564 ± 0.027	2.482LysTyr: 2.482 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	7.621LeuAla: 7.621 ± 0.131	1.621LeuCys: 1.621 ± 0.047	4.933LeuAsp: 4.933 ± 0.089	7.323LeuGlu: 7.323 ± 0.097	4.118LeuPhe: 4.118 ± 0.089	6.142LeuGly: 6.142 ± 0.107	1.696LeuHis: 1.696 ± 0.046	5.105LeuIle: 5.105 ± 0.088	5.227LeuLys: 5.227 ± 0.083	9.031LeuLeu: 9.031 ± 0.163	2.758LeuMet: 2.758 ± 0.054	2.997LeuAsn: 2.997 ± 0.07	3.938LeuPro: 3.938 ± 0.077	3.558LeuGln: 3.558 ± 0.088	4.257LeuArg: 4.257 ± 0.083	5.819LeuSer: 5.819 ± 0.081	5.535LeuThr: 5.535 ± 0.082	5.763LeuVal: 5.763 ± 0.1	0.907LeuTrp: 0.907 ± 0.041	3.145LeuTyr: 3.145 ± 0.066	0.0LeuXaa: 0.0 ± 0.0
Met	3.002MetAla: 3.002 ± 0.065	0.312MetCys: 0.312 ± 0.021	2.065MetAsp: 2.065 ± 0.047	3.203MetGlu: 3.203 ± 0.074	1.035MetPhe: 1.035 ± 0.033	2.515MetGly: 2.515 ± 0.058	0.466MetHis: 0.466 ± 0.026	1.922MetIle: 1.922 ± 0.052	2.47MetLys: 2.47 ± 0.054	2.761MetLeu: 2.761 ± 0.064	1.022MetMet: 1.022 ± 0.042	1.249MetAsn: 1.249 ± 0.042	1.333MetPro: 1.333 ± 0.045	1.317MetGln: 1.317 ± 0.046	1.397MetArg: 1.397 ± 0.04	1.596MetSer: 1.596 ± 0.047	1.946MetThr: 1.946 ± 0.048	2.089MetVal: 2.089 ± 0.052	0.208MetTrp: 0.208 ± 0.016	0.885MetTyr: 0.885 ± 0.038	0.001MetXaa: 0.001 ± 0.001
Asn	3.03AsnAla: 3.03 ± 0.073	0.64AsnCys: 0.64 ± 0.031	1.924AsnAsp: 1.924 ± 0.049	2.484AsnGlu: 2.484 ± 0.071	1.613AsnPhe: 1.613 ± 0.048	3.547AsnGly: 3.547 ± 0.075	0.826AsnHis: 0.826 ± 0.034	3.072AsnIle: 3.072 ± 0.074	2.181AsnLys: 2.181 ± 0.07	3.321AsnLeu: 3.321 ± 0.071	1.284AsnMet: 1.284 ± 0.04	1.556AsnAsn: 1.556 ± 0.056	1.905AsnPro: 1.905 ± 0.055	1.321AsnGln: 1.321 ± 0.045	1.773AsnArg: 1.773 ± 0.041	1.889AsnSer: 1.889 ± 0.05	2.249AsnThr: 2.249 ± 0.067	2.775AsnVal: 2.775 ± 0.057	0.409AsnTrp: 0.409 ± 0.024	1.427AsnTyr: 1.427 ± 0.052	0.0AsnXaa: 0.0 ± 0.0
Pro	2.961ProAla: 2.961 ± 0.067	0.469ProCys: 0.469 ± 0.025	2.108ProAsp: 2.108 ± 0.05	3.256ProGlu: 3.256 ± 0.065	1.743ProPhe: 1.743 ± 0.053	2.019ProGly: 2.019 ± 0.065	0.653ProHis: 0.653 ± 0.031	2.206ProIle: 2.206 ± 0.056	2.089ProLys: 2.089 ± 0.055	3.107ProLeu: 3.107 ± 0.065	1.119ProMet: 1.119 ± 0.04	1.288ProAsn: 1.288 ± 0.045	0.926ProPro: 0.926 ± 0.041	1.224ProGln: 1.224 ± 0.043	1.013ProArg: 1.013 ± 0.036	1.697ProSer: 1.697 ± 0.048	1.808ProThr: 1.808 ± 0.05	3.025ProVal: 3.025 ± 0.062	0.305ProTrp: 0.305 ± 0.022	1.504ProTyr: 1.504 ± 0.049	0.001ProXaa: 0.001 ± 0.001
Gln	2.782GlnAla: 2.782 ± 0.066	0.414GlnCys: 0.414 ± 0.025	1.616GlnAsp: 1.616 ± 0.051	3.081GlnGlu: 3.081 ± 0.078	1.218GlnPhe: 1.218 ± 0.043	1.976GlnGly: 1.976 ± 0.049	0.484GlnHis: 0.484 ± 0.025	2.468GlnIle: 2.468 ± 0.058	3.16GlnLys: 3.16 ± 0.075	2.758GlnLeu: 2.758 ± 0.064	1.3GlnMet: 1.3 ± 0.042	1.728GlnAsn: 1.728 ± 0.05	1.099GlnPro: 1.099 ± 0.039	1.406GlnGln: 1.406 ± 0.056	1.598GlnArg: 1.598 ± 0.048	1.514GlnSer: 1.514 ± 0.051	2.061GlnThr: 2.061 ± 0.055	2.23GlnVal: 2.23 ± 0.057	0.423GlnTrp: 0.423 ± 0.027	1.32GlnTyr: 1.32 ± 0.044	0.0GlnXaa: 0.0 ± 0.0
Arg	2.83ArgAla: 2.83 ± 0.068	0.589ArgCys: 0.589 ± 0.03	2.004ArgAsp: 2.004 ± 0.052	3.727ArgGlu: 3.727 ± 0.073	1.844ArgPhe: 1.844 ± 0.053	2.453ArgGly: 2.453 ± 0.052	0.809ArgHis: 0.809 ± 0.037	3.137ArgIle: 3.137 ± 0.063	3.437ArgLys: 3.437 ± 0.077	3.975ArgLeu: 3.975 ± 0.081	1.565ArgMet: 1.565 ± 0.044	1.846ArgAsn: 1.846 ± 0.054	1.324ArgPro: 1.324 ± 0.041	1.749ArgGln: 1.749 ± 0.056	2.239ArgArg: 2.239 ± 0.061	2.002ArgSer: 2.002 ± 0.051	2.188ArgThr: 2.188 ± 0.053	2.604ArgVal: 2.604 ± 0.058	0.367ArgTrp: 0.367 ± 0.022	1.688ArgTyr: 1.688 ± 0.045	0.001ArgXaa: 0.001 ± 0.001
Ser	4.256SerAla: 4.256 ± 0.068	0.856SerCys: 0.856 ± 0.038	2.74SerAsp: 2.74 ± 0.07	3.205SerGlu: 3.205 ± 0.067	2.498SerPhe: 2.498 ± 0.063	4.462SerGly: 4.462 ± 0.078	1.034SerHis: 1.034 ± 0.038	3.625SerIle: 3.625 ± 0.081	2.83SerLys: 2.83 ± 0.066	4.967SerLeu: 4.967 ± 0.102	1.77SerMet: 1.77 ± 0.047	1.912SerAsn: 1.912 ± 0.055	1.816SerPro: 1.816 ± 0.052	1.711SerGln: 1.711 ± 0.058	2.283SerArg: 2.283 ± 0.051	2.799SerSer: 2.799 ± 0.073	2.606SerThr: 2.606 ± 0.067	4.225SerVal: 4.225 ± 0.072	0.499SerTrp: 0.499 ± 0.024	2.051SerTyr: 2.051 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	5.27ThrAla: 5.27 ± 0.099	0.67ThrCys: 0.67 ± 0.025	3.239ThrAsp: 3.239 ± 0.068	4.522ThrGlu: 4.522 ± 0.083	2.423ThrPhe: 2.423 ± 0.054	4.712ThrGly: 4.712 ± 0.104	0.864ThrHis: 0.864 ± 0.035	3.732ThrIle: 3.732 ± 0.08	3.387ThrLys: 3.387 ± 0.069	5.113ThrLeu: 5.113 ± 0.083	1.66ThrMet: 1.66 ± 0.046	1.931ThrAsn: 1.931 ± 0.053	2.374ThrPro: 2.374 ± 0.05	1.569ThrGln: 1.569 ± 0.05	1.858ThrArg: 1.858 ± 0.049	2.611ThrSer: 2.611 ± 0.062	3.12ThrThr: 3.12 ± 0.088	4.74ThrVal: 4.74 ± 0.088	0.495ThrTrp: 0.495 ± 0.026	2.106ThrTyr: 2.106 ± 0.057	0.0ThrXaa: 0.0 ± 0.0
Val	5.539ValAla: 5.539 ± 0.09	1.33ValCys: 1.33 ± 0.045	3.872ValAsp: 3.872 ± 0.082	5.041ValGlu: 5.041 ± 0.095	3.323ValPhe: 3.323 ± 0.08	4.634ValGly: 4.634 ± 0.097	1.23ValHis: 1.23 ± 0.04	4.84ValIle: 4.84 ± 0.089	4.287ValLys: 4.287 ± 0.082	7.357ValLeu: 7.357 ± 0.12	2.181ValMet: 2.181 ± 0.055	2.62ValAsn: 2.62 ± 0.063	2.842ValPro: 2.842 ± 0.062	2.226ValGln: 2.226 ± 0.054	2.697ValArg: 2.697 ± 0.064	4.412ValSer: 4.412 ± 0.07	4.485ValThr: 4.485 ± 0.096	5.548ValVal: 5.548 ± 0.113	0.667ValTrp: 0.667 ± 0.032	2.813ValTyr: 2.813 ± 0.058	0.001ValXaa: 0.001 ± 0.001
Trp	0.593TrpAla: 0.593 ± 0.03	0.128TrpCys: 0.128 ± 0.013	0.661TrpAsp: 0.661 ± 0.035	0.743TrpGlu: 0.743 ± 0.033	0.39TrpPhe: 0.39 ± 0.024	0.628TrpGly: 0.628 ± 0.032	0.166TrpHis: 0.166 ± 0.018	0.623TrpIle: 0.623 ± 0.028	0.809TrpLys: 0.809 ± 0.035	0.893TrpLeu: 0.893 ± 0.037	0.324TrpMet: 0.324 ± 0.019	0.488TrpAsn: 0.488 ± 0.026	0.148TrpPro: 0.148 ± 0.015	0.47TrpGln: 0.47 ± 0.028	0.402TrpArg: 0.402 ± 0.022	0.439TrpSer: 0.439 ± 0.027	0.436TrpThr: 0.436 ± 0.024	0.599TrpVal: 0.599 ± 0.027	0.094TrpTrp: 0.094 ± 0.01	0.35TrpTyr: 0.35 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.792TyrAla: 2.792 ± 0.058	0.644TyrCys: 0.644 ± 0.031	2.587TyrAsp: 2.587 ± 0.055	2.821TyrGlu: 2.821 ± 0.067	1.83TyrPhe: 1.83 ± 0.05	3.014TyrGly: 3.014 ± 0.063	0.898TyrHis: 0.898 ± 0.037	2.437TyrIle: 2.437 ± 0.057	1.728TyrLys: 1.728 ± 0.052	3.665TyrLeu: 3.665 ± 0.073	1.035TyrMet: 1.035 ± 0.034	1.471TyrAsn: 1.471 ± 0.053	1.424TyrPro: 1.424 ± 0.043	1.326TyrGln: 1.326 ± 0.045	1.767TyrArg: 1.767 ± 0.043	1.773TyrSer: 1.773 ± 0.051	2.245TyrThr: 2.245 ± 0.053	2.475TyrVal: 2.475 ± 0.058	0.38TyrTrp: 0.38 ± 0.025	1.542TyrTyr: 1.542 ± 0.048	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.004XaaAla: 0.004 ± 0.002	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.012XaaXaa: 0.012 ± 0.005

Statistics based on 2676 proteins (766065 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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