Metallosphaera turreted icosahedral virus

Taxonomy: Viruses; unclassified archaeal viruses

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221ZTP5|A0A221ZTP5_9VIRU Uncharacterized protein OS=Metallosphaera turreted icosahedral virus OX=2023155 PE=4 SV=1
MM1 pKa = 7.52SCQTCGQQGVCYY13 pKa = 9.77VLEE16 pKa = 4.34HH17 pKa = 6.6VGTEE21 pKa = 4.1PIYY24 pKa = 10.74INGQQYY30 pKa = 9.79GQGDD34 pKa = 4.12FCFSSPEE41 pKa = 3.85LTIEE45 pKa = 4.19YY46 pKa = 10.3QGVQYY51 pKa = 10.52QIDD54 pKa = 3.77MSYY57 pKa = 11.42APTVQNIYY65 pKa = 10.14GCCPSGTTAKK75 pKa = 9.25LTTVTTIQGQNTPIFVPYY93 pKa = 10.28AVLVSQGTTSTSSTTPSVLSKK114 pKa = 10.71VPWWLIILLVILGAILLGGGNPP136 pKa = 3.7

Molecular weight: 14.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221ZTP7|A0A221ZTP7_9VIRU Structural protein c105 OS=Metallosphaera turreted icosahedral virus OX=2023155 PE=4 SV=1
MM1 pKa = 7.14VSNRR5 pKa = 11.84EE6 pKa = 3.74LAKK9 pKa = 10.58RR10 pKa = 11.84LSQSARR16 pKa = 11.84EE17 pKa = 4.06AWNSEE22 pKa = 3.05EE23 pKa = 3.86GMRR26 pKa = 11.84IRR28 pKa = 11.84EE29 pKa = 4.09EE30 pKa = 4.03LSADD34 pKa = 3.43SASYY38 pKa = 9.55GACLRR43 pKa = 11.84RR44 pKa = 11.84VMEE47 pKa = 4.38SGGGSRR53 pKa = 11.84GYY55 pKa = 10.24KK56 pKa = 9.8DD57 pKa = 3.3CARR60 pKa = 11.84RR61 pKa = 11.84SGIGTAFASVWGSTKK76 pKa = 10.48AYY78 pKa = 8.01GRR80 pKa = 11.84PMVEE84 pKa = 3.9VVARR88 pKa = 3.91

Molecular weight: 9.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

2883

40

368

137.3

14.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.209 ± 0.672

1.318 ± 0.287

4.301 ± 0.677

5.55 ± 0.983

2.775 ± 0.315

9.712 ± 1.789

1.075 ± 0.268

5.55 ± 0.431

4.683 ± 0.781

7.804 ± 0.586

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.636 ± 0.352

3.052 ± 0.297

5.689 ± 0.736

5.099 ± 0.563

4.197 ± 0.864

7.804 ± 0.762

8.221 ± 1.413

8.672 ± 0.607

1.422 ± 0.231

4.232 ± 0.555
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski