Armillaria ostoyae (Armillaria root rot fungus)
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22192 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A284QTQ0|A0A284QTQ0_ARMOS Uncharacterized protein OS=Armillaria ostoyae OX=47428 GN=ARMOST_03104 PE=4 SV=1
MM1 pKa = 8.03 DD2 pKa = 5.78 ALDD5 pKa = 4.36 EE6 pKa = 4.7 GSDD9 pKa = 3.48 VDD11 pKa = 3.77 VTLVHH16 pKa = 6.68 EE17 pKa = 4.33 MLLNSVISYY26 pKa = 9.82 LIEE29 pKa = 4.16 MDD31 pKa = 3.95 TEE33 pKa = 4.56 STSPTDD39 pKa = 2.78 VDD41 pKa = 5.42 LNHH44 pKa = 6.97 EE45 pKa = 4.6 DD46 pKa = 3.52 KK47 pKa = 11.37 DD48 pKa = 4.03 LVEE51 pKa = 4.17 MLDD54 pKa = 3.68 YY55 pKa = 11.1 LVAKK59 pKa = 10.09 AALHH63 pKa = 6.63 LDD65 pKa = 5.13 PIPEE69 pKa = 4.38 DD70 pKa = 4.03 DD71 pKa = 4.19 KK72 pKa = 11.9 LVDD75 pKa = 3.65 NEE77 pKa = 4.29 VDD79 pKa = 3.45 GKK81 pKa = 9.2 TDD83 pKa = 2.96 SSGDD87 pKa = 3.35 IEE89 pKa = 4.58 TWCCEE94 pKa = 4.16 VEE96 pKa = 4.48 SSCDD100 pKa = 3.75 DD101 pKa = 3.85 EE102 pKa = 6.96 LDD104 pKa = 3.83 LDD106 pKa = 4.03 IVPLLLCSPPYY117 pKa = 10.75 GEE119 pKa = 4.7 WDD121 pKa = 3.62 DD122 pKa = 4.32 GEE124 pKa = 4.22 PVINIWDD131 pKa = 3.59 VSLKK135 pKa = 10.73 HH136 pKa = 6.13 GLTLIEE142 pKa = 5.34 PPQPWDD148 pKa = 3.4 EE149 pKa = 4.11 RR150 pKa = 11.84 DD151 pKa = 3.5 EE152 pKa = 4.67 EE153 pKa = 4.32 EE154 pKa = 5.45 LEE156 pKa = 4.41 RR157 pKa = 11.84 PPPMQLLPFIPRR169 pKa = 11.84 EE170 pKa = 4.18 SIEE173 pKa = 4.23 PEE175 pKa = 3.72 AQRR178 pKa = 11.84 GLWFYY183 pKa = 11.75 SEE185 pKa = 4.92 LSNDD189 pKa = 3.6 YY190 pKa = 9.94 PHH192 pKa = 7.5 SFEE195 pKa = 5.0 EE196 pKa = 4.25 KK197 pKa = 9.36 FSFINSYY204 pKa = 9.59 GCKK207 pKa = 9.67 EE208 pKa = 4.1 SCHH211 pKa = 6.12 PCSFGDD217 pKa = 3.56 TVAQNVQQ224 pKa = 2.85
Molecular weight: 25.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.554
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.643
Rodwell 3.579
Grimsley 3.452
Solomon 3.732
Lehninger 3.681
Nozaki 3.846
DTASelect 4.05
Thurlkill 3.592
EMBOSS 3.656
Sillero 3.872
Patrickios 1.227
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.782
Protein with the highest isoelectric point:
>tr|A0A284SBF7|A0A284SBF7_ARMOS Uncharacterized protein OS=Armillaria ostoyae OX=47428 GN=ARMOST_21918 PE=4 SV=1
MM1 pKa = 7.93 RR2 pKa = 11.84 ARR4 pKa = 11.84 FRR6 pKa = 11.84 ASLHH10 pKa = 4.87 MIKK13 pKa = 10.22 RR14 pKa = 11.84 QRR16 pKa = 11.84 LLLL19 pKa = 4.14
Molecular weight: 2.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.193
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.974
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22192
0
22192
9048897
8
4981
407.8
45.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.789 ± 0.013
1.409 ± 0.006
5.851 ± 0.012
5.852 ± 0.015
3.892 ± 0.009
6.008 ± 0.015
2.653 ± 0.008
5.273 ± 0.012
4.611 ± 0.015
9.341 ± 0.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.172 ± 0.006
3.519 ± 0.008
6.358 ± 0.022
3.621 ± 0.009
6.068 ± 0.013
8.657 ± 0.02
6.137 ± 0.011
6.347 ± 0.012
1.557 ± 0.007
2.884 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here