Mycobacterium phage Batiatus
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1N1G5|A0A2P1N1G5_9CAUD Uncharacterized protein OS=Mycobacterium phage Batiatus OX=2126812 GN=80 PE=4 SV=1
MM1 pKa = 7.36 AHH3 pKa = 6.42 WKK5 pKa = 9.59 YY6 pKa = 8.91 WWTMPLLIAAGIIGPGLAAPEE27 pKa = 4.19 AKK29 pKa = 10.36 ADD31 pKa = 3.49 ITSDD35 pKa = 3.54 AFVMALDD42 pKa = 4.35 SEE44 pKa = 5.11 GITYY48 pKa = 10.39 SSKK51 pKa = 10.3 PAVINAGKK59 pKa = 10.01 AVCDD63 pKa = 3.93 VLDD66 pKa = 3.65 TGYY69 pKa = 9.51 TMYY72 pKa = 10.19 EE73 pKa = 3.7 ASVFVYY79 pKa = 10.8 NNSNLDD85 pKa = 3.99 LYY87 pKa = 11.33 DD88 pKa = 3.2 SGYY91 pKa = 10.79 FVGAATASFCPEE103 pKa = 4.1 HH104 pKa = 7.29 LSGTGWVV111 pKa = 3.57
Molecular weight: 11.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.126
IPC2_protein 4.279
IPC_protein 4.151
Toseland 3.961
ProMoST 4.317
Dawson 4.139
Bjellqvist 4.291
Wikipedia 4.088
Rodwell 3.986
Grimsley 3.872
Solomon 4.126
Lehninger 4.088
Nozaki 4.266
DTASelect 4.482
Thurlkill 4.012
EMBOSS 4.088
Sillero 4.279
Patrickios 0.248
IPC_peptide 4.126
IPC2_peptide 4.253
IPC2.peptide.svr19 4.176
Protein with the highest isoelectric point:
>tr|A0A2P1N166|A0A2P1N166_9CAUD Uncharacterized protein OS=Mycobacterium phage Batiatus OX=2126812 GN=54 PE=4 SV=1
MM1 pKa = 7.82 PSLGRR6 pKa = 11.84 QVKK9 pKa = 9.72 CAQSPKK15 pKa = 9.24 TSTGSGFYY23 pKa = 10.26 SFPGRR28 pKa = 11.84 PTMSTFPAPRR38 pKa = 11.84 TLTEE42 pKa = 5.09 RR43 pKa = 11.84 IQGAHH48 pKa = 6.7 LNLKK52 pKa = 9.83 LARR55 pKa = 11.84 QAGNPDD61 pKa = 3.49 IIAAAEE67 pKa = 4.34 RR68 pKa = 11.84 ILNQLVDD75 pKa = 4.1 RR76 pKa = 11.84 LPRR79 pKa = 11.84 STSQEE84 pKa = 3.91 KK85 pKa = 10.38
Molecular weight: 9.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.736
IPC_protein 10.774
Toseland 11.067
ProMoST 11.111
Dawson 11.096
Bjellqvist 10.891
Wikipedia 11.389
Rodwell 11.213
Grimsley 11.14
Solomon 11.359
Lehninger 11.316
Nozaki 11.052
DTASelect 10.891
Thurlkill 11.052
EMBOSS 11.491
Sillero 11.052
Patrickios 10.994
IPC_peptide 11.374
IPC2_peptide 10.116
IPC2.peptide.svr19 8.852
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
105
0
105
18481
30
1176
176.0
19.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.752 ± 0.496
1.147 ± 0.144
6.699 ± 0.188
5.757 ± 0.289
2.976 ± 0.192
8.842 ± 0.508
2.175 ± 0.172
4.388 ± 0.176
3.133 ± 0.196
7.391 ± 0.226
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.283 ± 0.12
3.312 ± 0.174
6.147 ± 0.197
3.355 ± 0.208
6.964 ± 0.402
6.093 ± 0.278
6.488 ± 0.258
7.224 ± 0.27
2.386 ± 0.154
2.489 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here