Yersinia phage vB_Yen_X1
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W0H6|A0A5Q2W0H6_9CAUD Uncharacterized protein OS=Yersinia phage vB_Yen_X1 OX=2663327 GN=X1_80 PE=4 SV=1
MM1 pKa = 7.78 ANNIIIPISSYY12 pKa = 10.64 PDD14 pKa = 2.97 QTFSIEE20 pKa = 4.04 LDD22 pKa = 4.16 GIPLQMRR29 pKa = 11.84 VYY31 pKa = 10.37 WSTFNDD37 pKa = 3.1 KK38 pKa = 9.43 MRR40 pKa = 11.84 QLVEE44 pKa = 4.21 EE45 pKa = 4.31 EE46 pKa = 4.5 NGPEE50 pKa = 4.73 IYY52 pKa = 10.4 GAWHH56 pKa = 6.23 MDD58 pKa = 2.71 IVGSDD63 pKa = 3.06 IEE65 pKa = 4.88 LYY67 pKa = 11.04 GLGIVQGCDD76 pKa = 2.65 IIEE79 pKa = 4.75 PYY81 pKa = 10.4 SIPEE85 pKa = 3.82 LGGFFVGNIEE95 pKa = 4.29 GKK97 pKa = 10.43 ASEE100 pKa = 4.11 ITYY103 pKa = 10.62 DD104 pKa = 3.58 SLGSEE109 pKa = 4.24 HH110 pKa = 7.68 VLMYY114 pKa = 10.27 ILKK117 pKa = 10.44 EE118 pKa = 3.93 NLQDD122 pKa = 4.28 FYY124 pKa = 11.38 TAIGYY129 pKa = 9.97 RR130 pKa = 11.84 NATII134 pKa = 3.89
Molecular weight: 15.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.922
IPC2_protein 4.101
IPC_protein 4.012
Toseland 3.834
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.859
Rodwell 3.846
Grimsley 3.745
Solomon 3.961
Lehninger 3.91
Nozaki 4.088
DTASelect 4.228
Thurlkill 3.859
EMBOSS 3.872
Sillero 4.126
Patrickios 0.477
IPC_peptide 3.961
IPC2_peptide 4.101
IPC2.peptide.svr19 4.027
Protein with the highest isoelectric point:
>tr|A0A5Q2W414|A0A5Q2W414_9CAUD Uncharacterized protein OS=Yersinia phage vB_Yen_X1 OX=2663327 GN=X1_24 PE=4 SV=1
MM1 pKa = 7.13 IHH3 pKa = 6.26 VSRR6 pKa = 11.84 VKK8 pKa = 10.86 GGFAAFGKK16 pKa = 10.34 FGAVFQTDD24 pKa = 2.95 KK25 pKa = 10.99 SGKK28 pKa = 9.18 VVNTNIPKK36 pKa = 10.58 NSDD39 pKa = 2.69 KK40 pKa = 10.61 YY41 pKa = 9.86 RR42 pKa = 11.84 EE43 pKa = 3.8 MRR45 pKa = 11.84 KK46 pKa = 9.28 RR47 pKa = 11.84 VSAYY51 pKa = 9.17 IKK53 pKa = 10.03 EE54 pKa = 4.45 YY55 pKa = 10.41 YY56 pKa = 9.83 AA57 pKa = 5.38
Molecular weight: 6.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.187
IPC2_protein 9.721
IPC_protein 9.809
Toseland 10.35
ProMoST 9.955
Dawson 10.496
Bjellqvist 10.145
Wikipedia 10.657
Rodwell 11.082
Grimsley 10.57
Solomon 10.526
Lehninger 10.511
Nozaki 10.306
DTASelect 10.145
Thurlkill 10.365
EMBOSS 10.73
Sillero 10.409
Patrickios 10.847
IPC_peptide 10.54
IPC2_peptide 8.682
IPC2.peptide.svr19 8.72
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
88
0
88
15243
34
700
173.2
19.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.496 ± 0.266
1.01 ± 0.112
6.856 ± 0.261
7.636 ± 0.26
3.634 ± 0.164
7.19 ± 0.327
1.876 ± 0.14
6.56 ± 0.24
7.249 ± 0.26
7.439 ± 0.219
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.208 ± 0.145
4.75 ± 0.23
3.359 ± 0.186
3.451 ± 0.189
4.992 ± 0.224
5.268 ± 0.201
5.412 ± 0.217
6.928 ± 0.242
1.483 ± 0.131
3.201 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here