Salmonella phage ViI

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Cvivirinae; Kuttervirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 208 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1XT25|E1XT25_BPSAV Uncharacterized protein OS=Salmonella phage ViI OX=1987993 GN=Vi01_016 PE=4 SV=1
MM1 pKa = 7.07FVYY4 pKa = 10.3FRR6 pKa = 11.84SLSLLAYY13 pKa = 9.0VYY15 pKa = 10.18WLFDD19 pKa = 3.22ILCPRR24 pKa = 11.84FIKK27 pKa = 10.83EE28 pKa = 3.39EE29 pKa = 4.09VAFVNYY35 pKa = 9.61EE36 pKa = 3.81GQEE39 pKa = 4.22DD40 pKa = 4.06VWIPLCALSDD50 pKa = 3.7VTEE53 pKa = 4.6LDD55 pKa = 3.62DD56 pKa = 3.85VGMVGTMRR64 pKa = 11.84SFNLFGFALFPKK76 pKa = 10.4LIGDD80 pKa = 4.35LRR82 pKa = 11.84PYY84 pKa = 10.52FPDD87 pKa = 3.55EE88 pKa = 4.01EE89 pKa = 4.59VEE91 pKa = 4.08AA92 pKa = 5.33

Molecular weight: 10.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1XTF5|E1XTF5_BPSAV Uncharacterized protein OS=Salmonella phage ViI OX=1987993 GN=Vi01_146c PE=4 SV=1
MM1 pKa = 6.75TTRR4 pKa = 11.84IPPQSIEE11 pKa = 3.46ISKK14 pKa = 10.12VVNIEE19 pKa = 3.56PRR21 pKa = 11.84RR22 pKa = 11.84GTRR25 pKa = 11.84KK26 pKa = 9.07PRR28 pKa = 11.84AGRR31 pKa = 11.84HH32 pKa = 4.6LTTKK36 pKa = 10.78LDD38 pKa = 3.71LLKK41 pKa = 10.8QKK43 pKa = 10.83FGGHH47 pKa = 5.04SVFTRR52 pKa = 11.84IRR54 pKa = 11.84NALKK58 pKa = 10.19EE59 pKa = 3.98GRR61 pKa = 11.84TEE63 pKa = 4.12LEE65 pKa = 3.91LYY67 pKa = 10.03RR68 pKa = 11.84PNGSTRR74 pKa = 11.84AYY76 pKa = 8.21QTTDD80 pKa = 2.69GLLEE84 pKa = 4.85LIRR87 pKa = 11.84LSGMNIEE94 pKa = 4.68PRR96 pKa = 11.84SSGTPLCSLYY106 pKa = 10.99VISNLGALL114 pKa = 4.07

Molecular weight: 12.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

208

0

208

48240

38

1612

231.9

26.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.704 ± 0.173

1.146 ± 0.075

6.364 ± 0.126

6.544 ± 0.189

4.252 ± 0.113

6.718 ± 0.203

1.899 ± 0.123

6.366 ± 0.123

6.291 ± 0.182

7.983 ± 0.165

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.935 ± 0.096

5.143 ± 0.128

3.883 ± 0.101

3.802 ± 0.101

5.023 ± 0.12

6.449 ± 0.158

5.987 ± 0.199

7.193 ± 0.148

1.385 ± 0.072

3.932 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski