Banana bunchy top virus (isolate Autralia) (BBTV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Mulpavirales; Nanoviridae; Babuvirus; Banana bunchy top virus

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q86567|MREP_BBTVA Master replication protein OS=Banana bunchy top virus (isolate Autralia) OX=645099 GN=DNA-R PE=3 SV=1
MM1 pKa = 7.33EE2 pKa = 4.97FWEE5 pKa = 4.67SSAMPDD11 pKa = 3.64DD12 pKa = 3.95VKK14 pKa = 11.31RR15 pKa = 11.84EE16 pKa = 3.72IKK18 pKa = 10.1EE19 pKa = 4.02IYY21 pKa = 9.15WEE23 pKa = 4.07DD24 pKa = 3.32RR25 pKa = 11.84KK26 pKa = 11.05KK27 pKa = 11.13LLFCQKK33 pKa = 9.66LKK35 pKa = 10.9SYY37 pKa = 9.52VRR39 pKa = 11.84RR40 pKa = 11.84ILVYY44 pKa = 10.64GDD46 pKa = 3.7QEE48 pKa = 4.09DD49 pKa = 3.97ALAGVKK55 pKa = 10.06DD56 pKa = 3.93MKK58 pKa = 10.59TSIIRR63 pKa = 11.84YY64 pKa = 8.87SEE66 pKa = 4.17YY67 pKa = 10.68LKK69 pKa = 10.7KK70 pKa = 10.21PCVVICCVSNKK81 pKa = 10.3SIVYY85 pKa = 9.52RR86 pKa = 11.84LNSMVFFYY94 pKa = 10.84HH95 pKa = 7.31EE96 pKa = 4.24YY97 pKa = 10.77LEE99 pKa = 4.32EE100 pKa = 5.46LGGDD104 pKa = 3.39YY105 pKa = 10.77SVYY108 pKa = 10.45QDD110 pKa = 4.41LYY112 pKa = 9.79CDD114 pKa = 3.93EE115 pKa = 4.72VLSSSSTEE123 pKa = 3.93EE124 pKa = 3.64EE125 pKa = 4.28DD126 pKa = 3.54VGVIYY131 pKa = 10.6RR132 pKa = 11.84NVIMASTQEE141 pKa = 3.96KK142 pKa = 10.32FSWSDD147 pKa = 3.2CQQIVISDD155 pKa = 3.82YY156 pKa = 11.66DD157 pKa = 3.66VTLLL161 pKa = 4.53

Molecular weight: 18.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q65386|CAPSD_BBTVA Capsid protein OS=Banana bunchy top virus (isolate Autralia) OX=645099 GN=DNA-S PE=3 SV=1
MM1 pKa = 7.74TEE3 pKa = 3.98GQGNRR8 pKa = 11.84STLFWLAQQDD18 pKa = 3.64TRR20 pKa = 11.84INLLIRR26 pKa = 11.84RR27 pKa = 11.84TRR29 pKa = 11.84GRR31 pKa = 11.84TEE33 pKa = 3.76ILLHH37 pKa = 6.45LYY39 pKa = 10.04KK40 pKa = 10.75YY41 pKa = 9.38PNPVKK46 pKa = 10.48DD47 pKa = 3.55NCSLSSVKK55 pKa = 10.45VVVLRR60 pKa = 11.84RR61 pKa = 11.84KK62 pKa = 9.07IASGDD67 pKa = 3.4RR68 pKa = 11.84RR69 pKa = 11.84NDD71 pKa = 2.89LHH73 pKa = 8.12LEE75 pKa = 3.99RR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84GNYY81 pKa = 7.47EE82 pKa = 3.56AYY84 pKa = 9.59IGYY87 pKa = 9.54LL88 pKa = 3.35

Molecular weight: 10.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1023

42

286

146.1

17.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.519 ± 0.373

2.933 ± 0.499

5.181 ± 1.02

7.136 ± 0.735

4.008 ± 0.523

5.767 ± 0.731

1.662 ± 0.315

6.647 ± 0.68

6.549 ± 0.925

7.722 ± 1.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.226 ± 0.504

3.91 ± 0.791

3.519 ± 0.513

2.933 ± 0.466

7.918 ± 1.18

7.331 ± 1.1

4.594 ± 0.803

7.918 ± 0.615

1.662 ± 0.108

5.865 ± 0.658
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski