Fusobacterium phage Funu1
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E3Y6D7|A0A0E3Y6D7_9CAUD Uncharacterized protein OS=Fusobacterium phage Funu1 OX=1640977 GN=HMPREF1993_00015 PE=4 SV=1
MM1 pKa = 7.7 KK2 pKa = 9.81 EE3 pKa = 4.09 FKK5 pKa = 10.08 MKK7 pKa = 10.53 AWLKK11 pKa = 10.63 KK12 pKa = 7.39 EE13 pKa = 3.82 NKK15 pKa = 7.95 MVSIIGIDD23 pKa = 3.64 LNYY26 pKa = 10.64 QYY28 pKa = 10.91 IRR30 pKa = 11.84 YY31 pKa = 9.46 SDD33 pKa = 4.19 DD34 pKa = 3.34 GNLFKK39 pKa = 10.76 DD40 pKa = 3.88 DD41 pKa = 3.72 YY42 pKa = 11.14 KK43 pKa = 10.79 IAEE46 pKa = 4.32 FKK48 pKa = 10.77 DD49 pKa = 3.25 IEE51 pKa = 4.22 LLQFTGAKK59 pKa = 9.85 DD60 pKa = 3.22 KK61 pKa = 11.31 AGQEE65 pKa = 4.2 VYY67 pKa = 10.06 EE68 pKa = 4.7 ADD70 pKa = 3.47 VIKK73 pKa = 10.93 FNDD76 pKa = 5.66 GIDD79 pKa = 4.32 DD80 pKa = 3.8 IYY82 pKa = 11.75 GLISYY87 pKa = 10.3 DD88 pKa = 4.47 DD89 pKa = 4.08 EE90 pKa = 4.98 DD91 pKa = 3.74 AVYY94 pKa = 10.22 CVSYY98 pKa = 11.25 EE99 pKa = 3.95 NVTEE103 pKa = 4.34 HH104 pKa = 7.06 LLNMAGNFEE113 pKa = 4.07 IVGNIFEE120 pKa = 4.68 NPNLHH125 pKa = 5.97 EE126 pKa = 4.09 QLGYY130 pKa = 11.04
Molecular weight: 15.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.263
IPC2_protein 4.329
IPC_protein 4.266
Toseland 4.075
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.126
Rodwell 4.101
Grimsley 3.986
Solomon 4.228
Lehninger 4.177
Nozaki 4.342
DTASelect 4.533
Thurlkill 4.101
EMBOSS 4.139
Sillero 4.38
Patrickios 3.3
IPC_peptide 4.228
IPC2_peptide 4.368
IPC2.peptide.svr19 4.31
Protein with the highest isoelectric point:
>tr|A0A0E3Y606|A0A0E3Y606_9CAUD Uncharacterized protein OS=Fusobacterium phage Funu1 OX=1640977 GN=HMPREF1993_00019 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 10.4 KK3 pKa = 10.07 KK4 pKa = 10.38 LRR6 pKa = 11.84 EE7 pKa = 3.88 LRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 10.01 KK12 pKa = 10.78 EE13 pKa = 3.78 FVKK16 pKa = 10.56 LCKK19 pKa = 10.07 GALQRR24 pKa = 11.84 NYY26 pKa = 10.44 VYY28 pKa = 11.03 GVFNRR33 pKa = 11.84 NCVFKK38 pKa = 10.93 RR39 pKa = 11.84 KK40 pKa = 9.3 GDD42 pKa = 3.33 KK43 pKa = 9.89 TRR45 pKa = 11.84 FKK47 pKa = 10.76 KK48 pKa = 10.27 GVKK51 pKa = 10.0 FMVLITLPKK60 pKa = 9.89 IINSCLNTKK69 pKa = 9.8 VKK71 pKa = 10.83 AKK73 pKa = 9.99 AKK75 pKa = 10.3 KK76 pKa = 10.09 RR77 pKa = 11.84 GGKK80 pKa = 8.77 NDD82 pKa = 3.31 KK83 pKa = 10.67
Molecular weight: 9.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.246
IPC2_protein 10.014
IPC_protein 10.438
Toseland 11.213
ProMoST 10.818
Dawson 11.257
Bjellqvist 10.906
Wikipedia 11.418
Rodwell 11.784
Grimsley 11.286
Solomon 11.374
Lehninger 11.359
Nozaki 11.184
DTASelect 10.906
Thurlkill 11.184
EMBOSS 11.608
Sillero 11.184
Patrickios 11.506
IPC_peptide 11.389
IPC2_peptide 9.706
IPC2.peptide.svr19 8.524
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
65
0
65
11841
33
915
182.2
20.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.966 ± 0.501
0.971 ± 0.132
5.312 ± 0.234
9.307 ± 0.49
4.569 ± 0.183
5.329 ± 0.43
1.022 ± 0.094
9.644 ± 0.327
12.018 ± 0.502
8.504 ± 0.254
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.12 ± 0.122
6.976 ± 0.267
2.415 ± 0.271
2.66 ± 0.135
3.26 ± 0.186
5.793 ± 0.229
4.983 ± 0.278
5.0 ± 0.206
0.937 ± 0.096
4.214 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here