Banna virus
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B4Y052|B4Y052_9REOV VP8 OS=Banna virus OX=77763 PE=4 SV=1
MM1 pKa = 7.58 NNGQATITRR10 pKa = 11.84 IGRR13 pKa = 11.84 NFEE16 pKa = 3.56 IRR18 pKa = 11.84 CRR20 pKa = 11.84 HH21 pKa = 6.31 LDD23 pKa = 3.38 RR24 pKa = 11.84 DD25 pKa = 3.97 YY26 pKa = 11.43 TLPLPNATSNDD37 pKa = 3.34 NFLDD41 pKa = 4.43 CIKK44 pKa = 10.76 FITEE48 pKa = 4.04 CVGFNYY54 pKa = 9.95 VSSGFKK60 pKa = 10.61 LSIDD64 pKa = 3.39 INDD67 pKa = 3.76 FQHH70 pKa = 6.83 FNGNSTLLIGKK81 pKa = 6.81 TKK83 pKa = 10.22 IGPLILKK90 pKa = 9.61 KK91 pKa = 10.56 VRR93 pKa = 11.84 SLPCCNDD100 pKa = 2.73 ALFRR104 pKa = 11.84 NEE106 pKa = 3.65 FRR108 pKa = 11.84 ILAKK112 pKa = 9.4 MHH114 pKa = 6.73 GILRR118 pKa = 11.84 LKK120 pKa = 10.8 NDD122 pKa = 3.56 INGHH126 pKa = 5.51 KK127 pKa = 10.31 YY128 pKa = 9.91 GVILEE133 pKa = 4.27 RR134 pKa = 11.84 CYY136 pKa = 10.84 KK137 pKa = 10.5 PNINFCNFVTAINDD151 pKa = 3.58 LEE153 pKa = 4.48 VFHH156 pKa = 7.05 SSNPHH161 pKa = 5.99 FLHH164 pKa = 7.5 GDD166 pKa = 3.65 ANPDD170 pKa = 3.6 NIMSDD175 pKa = 2.81 SDD177 pKa = 4.39 GYY179 pKa = 11.32 LKK181 pKa = 10.89 LVDD184 pKa = 4.19 PVCLLEE190 pKa = 4.24 NQVNMVNVDD199 pKa = 3.77 YY200 pKa = 11.23 EE201 pKa = 4.54 SLTQEE206 pKa = 3.9 AEE208 pKa = 3.77 KK209 pKa = 10.82 KK210 pKa = 10.5 VFVKK214 pKa = 10.73 SLLLLVEE221 pKa = 4.43 KK222 pKa = 10.44 QLSASADD229 pKa = 3.63 EE230 pKa = 4.89 IYY232 pKa = 11.55 VNLKK236 pKa = 7.26 EE237 pKa = 4.53 TNPSFNLEE245 pKa = 4.29 CGLKK249 pKa = 9.49 LTDD252 pKa = 4.93 LLDD255 pKa = 5.21 SIDD258 pKa = 3.76 THH260 pKa = 8.08 NSIHH264 pKa = 6.23 WKK266 pKa = 10.7 SMLNYY271 pKa = 9.51 QPIMPEE277 pKa = 3.89 LSVLNDD283 pKa = 3.14 LTYY286 pKa = 11.22 YY287 pKa = 9.89 DD288 pKa = 3.87 TGDD291 pKa = 3.55 VKK293 pKa = 11.36 DD294 pKa = 5.33 LVTEE298 pKa = 4.51 DD299 pKa = 5.48 LDD301 pKa = 6.27 DD302 pKa = 4.69 EE303 pKa = 5.88 DD304 pKa = 5.88 DD305 pKa = 3.7 VV306 pKa = 4.58
Molecular weight: 34.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.8
IPC2_protein 4.863
IPC_protein 4.825
Toseland 4.724
ProMoST 4.991
Dawson 4.838
Bjellqvist 4.965
Wikipedia 4.736
Rodwell 4.711
Grimsley 4.647
Solomon 4.825
Lehninger 4.787
Nozaki 4.952
DTASelect 5.169
Thurlkill 4.749
EMBOSS 4.774
Sillero 5.003
Patrickios 3.706
IPC_peptide 4.838
IPC2_peptide 4.991
IPC2.peptide.svr19 4.969
Protein with the highest isoelectric point:
>tr|B4Y055|B4Y055_9REOV VP11 OS=Banna virus OX=77763 PE=4 SV=1
MM1 pKa = 7.93 DD2 pKa = 4.23 VLSKK6 pKa = 11.18 SSLKK10 pKa = 10.48 EE11 pKa = 3.7 LLAHH15 pKa = 7.03 LEE17 pKa = 4.06 KK18 pKa = 10.52 TPLEE22 pKa = 3.95 EE23 pKa = 5.08 AISYY27 pKa = 10.23 KK28 pKa = 10.25 IGTIPYY34 pKa = 8.23 QNVFISRR41 pKa = 11.84 NEE43 pKa = 4.28 HH44 pKa = 5.74 YY45 pKa = 10.61 NQLYY49 pKa = 10.0 PDD51 pKa = 3.95 TTSLIDD57 pKa = 3.54 GVSRR61 pKa = 11.84 EE62 pKa = 4.0 GQRR65 pKa = 11.84 NVNGLIMSIISYY77 pKa = 9.13 VVSGSGHH84 pKa = 6.27 YY85 pKa = 9.77 IPNIGSMLLRR95 pKa = 11.84 RR96 pKa = 11.84 SILDD100 pKa = 3.1 ILTKK104 pKa = 10.3 HH105 pKa = 6.06 DD106 pKa = 3.68 TGLVTNNLNYY116 pKa = 10.81 GIIARR121 pKa = 11.84 NLTVNKK127 pKa = 9.03 MNCEE131 pKa = 3.59 QRR133 pKa = 11.84 KK134 pKa = 9.66 RR135 pKa = 11.84 MLICFKK141 pKa = 10.89 LLAYY145 pKa = 9.8 KK146 pKa = 10.52 DD147 pKa = 4.31 GNQNDD152 pKa = 3.94 YY153 pKa = 11.14 EE154 pKa = 4.51 VYY156 pKa = 10.56 LNQNIPLKK164 pKa = 10.6 QIAPSFIPNDD174 pKa = 3.12 MRR176 pKa = 11.84 TVINNNDD183 pKa = 3.09 QLGIVGIPVYY193 pKa = 10.52 RR194 pKa = 11.84 LTQSTEE200 pKa = 3.33 LSIRR204 pKa = 11.84 DD205 pKa = 4.2 DD206 pKa = 3.48 NAKK209 pKa = 10.12 SYY211 pKa = 11.21 KK212 pKa = 10.04 LGYY215 pKa = 9.08 VDD217 pKa = 4.72 WYY219 pKa = 10.85 NSNAFLRR226 pKa = 11.84 EE227 pKa = 3.87 RR228 pKa = 11.84 NDD230 pKa = 3.35 FNLLKK235 pKa = 10.89 LKK237 pKa = 10.45 DD238 pKa = 3.42 RR239 pKa = 11.84 NTRR242 pKa = 11.84 YY243 pKa = 10.27 GRR245 pKa = 11.84 LIGWW249 pKa = 3.97
Molecular weight: 28.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.623
IPC2_protein 8.653
IPC_protein 8.624
Toseland 8.99
ProMoST 8.946
Dawson 9.355
Bjellqvist 9.18
Wikipedia 9.575
Rodwell 9.458
Grimsley 9.443
Solomon 9.428
Lehninger 9.37
Nozaki 9.121
DTASelect 9.136
Thurlkill 9.165
EMBOSS 9.443
Sillero 9.326
Patrickios 4.304
IPC_peptide 9.414
IPC2_peptide 7.892
IPC2.peptide.svr19 7.755
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
5979
184
1219
498.3
56.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.138 ± 0.638
1.254 ± 0.199
6.673 ± 0.358
5.135 ± 0.211
3.897 ± 0.243
5.503 ± 0.253
2.342 ± 0.222
6.857 ± 0.286
6.573 ± 0.37
9.282 ± 0.389
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.726 ± 0.112
6.958 ± 0.371
3.278 ± 0.315
3.061 ± 0.38
4.7 ± 0.284
7.242 ± 0.456
6.004 ± 0.504
7.459 ± 0.295
0.669 ± 0.125
4.248 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here