Simian adenovirus 24
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6QP99|Q6QP99_9ADEN Isoform of Q6QPA1 Pre-hexon-linking protein VIII OS=Simian adenovirus 24 OX=175568 GN=L4 PE=2 SV=1
MM1 pKa = 7.54 RR2 pKa = 11.84 HH3 pKa = 6.29 LRR5 pKa = 11.84 DD6 pKa = 3.2 LPGNVFLATGNEE18 pKa = 3.79 ILEE21 pKa = 4.42 LVVDD25 pKa = 4.42 AMMGGDD31 pKa = 3.95 PPEE34 pKa = 4.7 PPTPFEE40 pKa = 4.46 APSLYY45 pKa = 10.52 DD46 pKa = 4.21 LYY48 pKa = 11.22 DD49 pKa = 4.17 LEE51 pKa = 4.96 VDD53 pKa = 4.44 VPEE56 pKa = 4.6 NDD58 pKa = 3.8 PNEE61 pKa = 4.16 EE62 pKa = 3.91 AVNDD66 pKa = 4.14 LFSDD70 pKa = 4.25 AALLAAEE77 pKa = 4.49 QANTDD82 pKa = 3.78 SGSDD86 pKa = 3.51 SDD88 pKa = 5.19 SSLHH92 pKa = 5.2 TPRR95 pKa = 11.84 PGRR98 pKa = 11.84 GEE100 pKa = 3.95 KK101 pKa = 10.14 KK102 pKa = 9.74 IPEE105 pKa = 4.3 LKK107 pKa = 10.87 GEE109 pKa = 4.08 EE110 pKa = 4.1 LDD112 pKa = 3.62 LRR114 pKa = 11.84 CYY116 pKa = 9.99 EE117 pKa = 4.13 EE118 pKa = 5.15 CLPPSDD124 pKa = 5.59 DD125 pKa = 4.51 EE126 pKa = 4.45 EE127 pKa = 5.74 DD128 pKa = 3.72 EE129 pKa = 4.2 EE130 pKa = 5.51 AIRR133 pKa = 11.84 AASNQGVKK141 pKa = 10.32 AAGEE145 pKa = 4.49 SFSLDD150 pKa = 3.7 CPTLPGHH157 pKa = 5.87 GCKK160 pKa = 9.99 SCEE163 pKa = 3.94 FHH165 pKa = 7.8 RR166 pKa = 11.84 MNTGDD171 pKa = 4.13 KK172 pKa = 11.02 NVMCALCYY180 pKa = 9.23 MRR182 pKa = 11.84 AYY184 pKa = 8.98 NHH186 pKa = 6.34 CVYY189 pKa = 11.0 SPVSDD194 pKa = 3.54 VDD196 pKa = 3.5 EE197 pKa = 4.95 TPTSEE202 pKa = 5.04 CISSPPEE209 pKa = 3.91 IGEE212 pKa = 4.39 EE213 pKa = 3.96 PPEE216 pKa = 4.78 DD217 pKa = 4.09 IIHH220 pKa = 6.56 RR221 pKa = 11.84 PVAVRR226 pKa = 11.84 VTGRR230 pKa = 11.84 RR231 pKa = 11.84 AAVEE235 pKa = 4.11 SLDD238 pKa = 5.42 DD239 pKa = 4.14 LLQGGDD245 pKa = 4.15 EE246 pKa = 4.44 PLDD249 pKa = 3.5 LCTRR253 pKa = 11.84 KK254 pKa = 9.93 RR255 pKa = 11.84 PRR257 pKa = 11.84 HH258 pKa = 4.74
Molecular weight: 28.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.257
IPC2_protein 4.266
IPC_protein 4.228
Toseland 4.05
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.062
Rodwell 4.062
Grimsley 3.961
Solomon 4.177
Lehninger 4.139
Nozaki 4.291
DTASelect 4.469
Thurlkill 4.062
EMBOSS 4.075
Sillero 4.342
Patrickios 3.363
IPC_peptide 4.19
IPC2_peptide 4.329
IPC2.peptide.svr19 4.271
Protein with the highest isoelectric point:
>tr|Q6QPA7|Q6QPA7_9ADEN V OS=Simian adenovirus 24 OX=175568 PE=3 SV=1
MM1 pKa = 7.77 ALTCRR6 pKa = 11.84 LRR8 pKa = 11.84 VPITGYY14 pKa = 10.11 RR15 pKa = 11.84 GRR17 pKa = 11.84 KK18 pKa = 6.61 PRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 LTGNGLRR30 pKa = 11.84 RR31 pKa = 11.84 HH32 pKa = 4.44 HH33 pKa = 6.27 HH34 pKa = 5.26 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 AISKK42 pKa = 9.94 RR43 pKa = 11.84 LGGGFLPALIPIIAAAIGAIPGIASVAVQASQRR76 pKa = 11.84 HH77 pKa = 4.3
Molecular weight: 8.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.412
IPC2_protein 11.023
IPC_protein 12.486
Toseland 12.632
ProMoST 13.144
Dawson 12.647
Bjellqvist 12.632
Wikipedia 13.115
Rodwell 12.193
Grimsley 12.676
Solomon 13.144
Lehninger 13.042
Nozaki 12.632
DTASelect 12.632
Thurlkill 12.632
EMBOSS 13.144
Sillero 12.632
Patrickios 11.93
IPC_peptide 13.144
IPC2_peptide 12.135
IPC2.peptide.svr19 9.126
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
32
4
36
11588
77
1190
321.9
36.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.034 ± 0.347
2.028 ± 0.306
4.953 ± 0.314
6.386 ± 0.523
3.573 ± 0.213
6.343 ± 0.294
2.416 ± 0.229
3.806 ± 0.276
3.573 ± 0.381
9.631 ± 0.336
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.58 ± 0.185
4.177 ± 0.34
6.792 ± 0.303
4.332 ± 0.293
7.396 ± 0.6
6.938 ± 0.43
6.162 ± 0.46
6.369 ± 0.268
1.277 ± 0.093
3.236 ± 0.32
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here