Ulvibacter antarcticus
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3363 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3L9YW39|A0A3L9YW39_9FLAO Uncharacterized protein OS=Ulvibacter antarcticus OX=442714 GN=BXY75_2057 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.72 NSILCAAALLIGTAGIAQLNIANIVQTGLNNNSNNVQNGVYY43 pKa = 10.58 NEE45 pKa = 4.0 IKK47 pKa = 10.5 VIQNGNSNNSLIAQNGTNNFSCVEE71 pKa = 3.86 QDD73 pKa = 3.29 GDD75 pKa = 3.62 WNYY78 pKa = 11.17 SEE80 pKa = 5.35 VDD82 pKa = 2.92 QTGNYY87 pKa = 8.71 NSNVGLQQGDD97 pKa = 4.31 LNFSDD102 pKa = 4.38 VDD104 pKa = 3.74 QDD106 pKa = 3.79 GDD108 pKa = 3.85 NNNADD113 pKa = 3.99 FDD115 pKa = 4.11 QLGNQNANAQNQLGDD130 pKa = 3.89 NNDD133 pKa = 3.44 SQVYY137 pKa = 10.7 SDD139 pKa = 4.0 GNMNATATDD148 pKa = 3.45 QQGDD152 pKa = 4.01 SNSAFVYY159 pKa = 10.39 QDD161 pKa = 3.03 GNQNVGDD168 pKa = 4.03 IAQSGDD174 pKa = 3.47 SNLGYY179 pKa = 10.13 IYY181 pKa = 10.35 QDD183 pKa = 3.24 GNKK186 pKa = 9.91 NSGDD190 pKa = 3.57 IEE192 pKa = 4.18 QSGDD196 pKa = 3.41 SNQAEE201 pKa = 3.81 ILQFGNRR208 pKa = 11.84 NASAVTQTGDD218 pKa = 2.98 ANFAVTIQTDD228 pKa = 3.14 RR229 pKa = 11.84 RR230 pKa = 11.84 NVSNVEE236 pKa = 3.7 QTGDD240 pKa = 3.37 GNFAFVQQDD249 pKa = 2.98 GRR251 pKa = 11.84 RR252 pKa = 11.84 NSSDD256 pKa = 2.83 INQTGDD262 pKa = 3.24 GNYY265 pKa = 9.0 STVSQTGRR273 pKa = 11.84 KK274 pKa = 7.92 NVNYY278 pKa = 10.31 NSQQGNYY285 pKa = 9.05 NSNDD289 pKa = 2.45 ITQIGRR295 pKa = 11.84 RR296 pKa = 11.84 NDD298 pKa = 3.32 SYY300 pKa = 11.79 VLQLGDD306 pKa = 3.67 YY307 pKa = 9.82 NKK309 pKa = 9.03 NTTVQDD315 pKa = 4.66 GINNDD320 pKa = 3.03 SDD322 pKa = 4.15 VYY324 pKa = 11.09 QIGDD328 pKa = 3.96 GNCSDD333 pKa = 4.51 VFQDD337 pKa = 3.45 GNNNVGLTVQVGNNHH352 pKa = 6.81 IANIHH357 pKa = 4.18 QTGNGNNATIGQLGGTPFNTTITQTGGGNLTKK389 pKa = 10.17 INQNGSFF396 pKa = 3.6
Molecular weight: 42.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.666
IPC2_protein 3.681
IPC_protein 3.732
Toseland 3.478
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.732
Rodwell 3.541
Grimsley 3.389
Solomon 3.745
Lehninger 3.694
Nozaki 3.859
DTASelect 4.19
Thurlkill 3.554
EMBOSS 3.732
Sillero 3.859
Patrickios 1.214
IPC_peptide 3.732
IPC2_peptide 3.821
IPC2.peptide.svr19 3.761
Protein with the highest isoelectric point:
>tr|A0A3L9YVR7|A0A3L9YVR7_9FLAO YHYH protein OS=Ulvibacter antarcticus OX=442714 GN=BXY75_1647 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.18 RR22 pKa = 11.84 MASVNGRR29 pKa = 11.84 KK30 pKa = 9.17 VLAARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.87 GRR40 pKa = 11.84 KK41 pKa = 8.56 KK42 pKa = 10.21 ISVSSEE48 pKa = 3.74 TRR50 pKa = 11.84 HH51 pKa = 5.96 KK52 pKa = 10.68 KK53 pKa = 9.8
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3363
0
3363
1143827
25
3224
340.1
38.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.506 ± 0.04
0.774 ± 0.015
5.715 ± 0.035
6.66 ± 0.042
5.15 ± 0.033
6.676 ± 0.054
1.694 ± 0.023
7.93 ± 0.04
7.243 ± 0.057
9.238 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.254 ± 0.025
6.09 ± 0.051
3.391 ± 0.027
3.234 ± 0.027
3.415 ± 0.027
6.839 ± 0.034
5.977 ± 0.046
6.293 ± 0.037
0.994 ± 0.014
3.926 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here