Amino acid dipepetide frequency for Shewanella sp. OPT22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.489AlaAla: 6.489 ± 0.095
0.918AlaCys: 0.918 ± 0.026
4.328AlaAsp: 4.328 ± 0.065
5.363AlaGlu: 5.363 ± 0.076
3.177AlaPhe: 3.177 ± 0.061
5.126AlaGly: 5.126 ± 0.089
1.57AlaHis: 1.57 ± 0.038
5.751AlaIle: 5.751 ± 0.075
5.346AlaLys: 5.346 ± 0.076
8.521AlaLeu: 8.521 ± 0.091
2.116AlaMet: 2.116 ± 0.048
3.584AlaAsn: 3.584 ± 0.06
2.553AlaPro: 2.553 ± 0.048
3.42AlaGln: 3.42 ± 0.057
2.933AlaArg: 2.933 ± 0.049
5.266AlaSer: 5.266 ± 0.066
4.042AlaThr: 4.042 ± 0.055
5.186AlaVal: 5.186 ± 0.067
0.771AlaTrp: 0.771 ± 0.024
2.193AlaTyr: 2.193 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
0.711CysAla: 0.711 ± 0.025
0.174CysCys: 0.174 ± 0.013
0.598CysAsp: 0.598 ± 0.02
0.638CysGlu: 0.638 ± 0.023
0.567CysPhe: 0.567 ± 0.02
0.798CysGly: 0.798 ± 0.03
0.343CysHis: 0.343 ± 0.017
0.67CysIle: 0.67 ± 0.024
0.593CysLys: 0.593 ± 0.024
0.98CysLeu: 0.98 ± 0.027
0.215CysMet: 0.215 ± 0.013
0.481CysAsn: 0.481 ± 0.021
0.422CysPro: 0.422 ± 0.018
0.439CysGln: 0.439 ± 0.018
0.412CysArg: 0.412 ± 0.017
0.832CysSer: 0.832 ± 0.031
0.463CysThr: 0.463 ± 0.016
0.592CysVal: 0.592 ± 0.023
0.111CysTrp: 0.111 ± 0.01
0.333CysTyr: 0.333 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
4.289AspAla: 4.289 ± 0.07
0.56AspCys: 0.56 ± 0.021
3.323AspAsp: 3.323 ± 0.069
3.943AspGlu: 3.943 ± 0.069
2.686AspPhe: 2.686 ± 0.045
3.799AspGly: 3.799 ± 0.085
1.087AspHis: 1.087 ± 0.028
4.162AspIle: 4.162 ± 0.07
3.677AspLys: 3.677 ± 0.059
5.26AspLeu: 5.26 ± 0.062
1.283AspMet: 1.283 ± 0.034
2.812AspAsn: 2.812 ± 0.054
1.905AspPro: 1.905 ± 0.041
1.739AspGln: 1.739 ± 0.035
2.1AspArg: 2.1 ± 0.041
3.78AspSer: 3.78 ± 0.069
2.82AspThr: 2.82 ± 0.057
3.987AspVal: 3.987 ± 0.063
0.792AspTrp: 0.792 ± 0.025
2.04AspTyr: 2.04 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
4.894GluAla: 4.894 ± 0.073
0.58GluCys: 0.58 ± 0.023
3.071GluAsp: 3.071 ± 0.06
3.538GluGlu: 3.538 ± 0.076
2.784GluPhe: 2.784 ± 0.053
3.417GluGly: 3.417 ± 0.055
1.716GluHis: 1.716 ± 0.035
3.961GluIle: 3.961 ± 0.055
4.184GluLys: 4.184 ± 0.071
7.573GluLeu: 7.573 ± 0.094
1.567GluMet: 1.567 ± 0.038
3.075GluAsn: 3.075 ± 0.054
2.034GluPro: 2.034 ± 0.041
4.023GluGln: 4.023 ± 0.056
2.985GluArg: 2.985 ± 0.052
4.196GluSer: 4.196 ± 0.078
3.338GluThr: 3.338 ± 0.045
4.337GluVal: 4.337 ± 0.065
0.633GluTrp: 0.633 ± 0.019
1.962GluTyr: 1.962 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.329PheAla: 3.329 ± 0.057
0.548PheCys: 0.548 ± 0.024
2.964PheAsp: 2.964 ± 0.055
2.817PheGlu: 2.817 ± 0.048
1.831PhePhe: 1.831 ± 0.041
3.052PheGly: 3.052 ± 0.061
0.882PheHis: 0.882 ± 0.025
3.13PheIle: 3.13 ± 0.059
2.614PheLys: 2.614 ± 0.056
3.465PheLeu: 3.465 ± 0.064
1.008PheMet: 1.008 ± 0.03
2.412PheAsn: 2.412 ± 0.053
1.4PhePro: 1.4 ± 0.036
1.338PheGln: 1.338 ± 0.03
1.466PheArg: 1.466 ± 0.035
3.739PheSer: 3.739 ± 0.06
2.517PheThr: 2.517 ± 0.047
2.793PheVal: 2.793 ± 0.049
0.539PheTrp: 0.539 ± 0.021
1.495PheTyr: 1.495 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
4.692GlyAla: 4.692 ± 0.079
0.775GlyCys: 0.775 ± 0.026
3.573GlyAsp: 3.573 ± 0.079
4.101GlyGlu: 4.101 ± 0.062
3.241GlyPhe: 3.241 ± 0.055
4.28GlyGly: 4.28 ± 0.085
1.533GlyHis: 1.533 ± 0.038
4.402GlyIle: 4.402 ± 0.062
4.175GlyLys: 4.175 ± 0.068
6.316GlyLeu: 6.316 ± 0.085
1.702GlyMet: 1.702 ± 0.043
2.992GlyAsn: 2.992 ± 0.077
1.365GlyPro: 1.365 ± 0.037
2.522GlyGln: 2.522 ± 0.05
2.72GlyArg: 2.72 ± 0.049
4.152GlySer: 4.152 ± 0.069
3.341GlyThr: 3.341 ± 0.068
4.68GlyVal: 4.68 ± 0.063
0.742GlyTrp: 0.742 ± 0.024
2.389GlyTyr: 2.389 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
1.399HisAla: 1.399 ± 0.035
0.339HisCys: 0.339 ± 0.017
1.18HisAsp: 1.18 ± 0.03
1.196HisGlu: 1.196 ± 0.031
1.227HisPhe: 1.227 ± 0.031
1.456HisGly: 1.456 ± 0.039
0.686HisHis: 0.686 ± 0.023
1.515HisIle: 1.515 ± 0.037
1.323HisLys: 1.323 ± 0.037
2.237HisLeu: 2.237 ± 0.043
0.456HisMet: 0.456 ± 0.019
1.028HisAsn: 1.028 ± 0.025
1.049HisPro: 1.049 ± 0.025
1.288HisGln: 1.288 ± 0.033
1.03HisArg: 1.03 ± 0.027
1.644HisSer: 1.644 ± 0.035
1.068HisThr: 1.068 ± 0.029
1.18HisVal: 1.18 ± 0.033
0.368HisTrp: 0.368 ± 0.018
0.897HisTyr: 0.897 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
6.007IleAla: 6.007 ± 0.071
0.698IleCys: 0.698 ± 0.023
4.46IleAsp: 4.46 ± 0.07
5.043IleGlu: 5.043 ± 0.066
2.451IlePhe: 2.451 ± 0.051
4.495IleGly: 4.495 ± 0.068
1.329IleHis: 1.329 ± 0.035
3.95IleIle: 3.95 ± 0.065
4.337IleLys: 4.337 ± 0.063
5.252IleLeu: 5.252 ± 0.064
1.23IleMet: 1.23 ± 0.034
3.584IleAsn: 3.584 ± 0.059
2.648IlePro: 2.648 ± 0.045
2.442IleGln: 2.442 ± 0.041
2.613IleArg: 2.613 ± 0.052
5.166IleSer: 5.166 ± 0.068
3.851IleThr: 3.851 ± 0.06
3.967IleVal: 3.967 ± 0.057
0.633IleTrp: 0.633 ± 0.026
1.827IleTyr: 1.827 ± 0.041
0.0IleXaa: 0.0 ± 0.0
Lys
5.357LysAla: 5.357 ± 0.072
0.464LysCys: 0.464 ± 0.021
3.23LysAsp: 3.23 ± 0.057
3.701LysGlu: 3.701 ± 0.061
2.161LysPhe: 2.161 ± 0.04
3.729LysGly: 3.729 ± 0.065
1.541LysHis: 1.541 ± 0.034
3.541LysIle: 3.541 ± 0.054
3.974LysLys: 3.974 ± 0.066
6.816LysLeu: 6.816 ± 0.086
1.56LysMet: 1.56 ± 0.037
2.926LysAsn: 2.926 ± 0.049
2.654LysPro: 2.654 ± 0.048
3.674LysGln: 3.674 ± 0.054
2.87LysArg: 2.87 ± 0.048
4.303LysSer: 4.303 ± 0.06
3.388LysThr: 3.388 ± 0.052
4.606LysVal: 4.606 ± 0.065
0.618LysTrp: 0.618 ± 0.023
1.855LysTyr: 1.855 ± 0.042
0.0LysXaa: 0.0 ± 0.0
Leu
8.462LeuAla: 8.462 ± 0.1
1.066LeuCys: 1.066 ± 0.032
5.484LeuAsp: 5.484 ± 0.07
6.267LeuGlu: 6.267 ± 0.081
4.245LeuPhe: 4.245 ± 0.065
6.265LeuGly: 6.265 ± 0.08
1.995LeuHis: 1.995 ± 0.037
6.499LeuIle: 6.499 ± 0.079
6.636LeuLys: 6.636 ± 0.083
10.114LeuLeu: 10.114 ± 0.139
2.539LeuMet: 2.539 ± 0.053
5.3LeuAsn: 5.3 ± 0.067
4.549LeuPro: 4.549 ± 0.062
3.689LeuGln: 3.689 ± 0.058
3.803LeuArg: 3.803 ± 0.058
8.38LeuSer: 8.38 ± 0.104
6.151LeuThr: 6.151 ± 0.067
6.385LeuVal: 6.385 ± 0.078
0.953LeuTrp: 0.953 ± 0.028
2.601LeuTyr: 2.601 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
2.107MetAla: 2.107 ± 0.048
0.21MetCys: 0.21 ± 0.013
1.14MetAsp: 1.14 ± 0.03
1.17MetGlu: 1.17 ± 0.031
0.913MetPhe: 0.913 ± 0.026
1.455MetGly: 1.455 ± 0.034
0.45MetHis: 0.45 ± 0.018
1.41MetIle: 1.41 ± 0.038
1.649MetLys: 1.649 ± 0.04
2.603MetLeu: 2.603 ± 0.053
0.715MetMet: 0.715 ± 0.025
1.156MetAsn: 1.156 ± 0.032
1.097MetPro: 1.097 ± 0.031
1.046MetGln: 1.046 ± 0.029
1.03MetArg: 1.03 ± 0.029
1.914MetSer: 1.914 ± 0.037
1.438MetThr: 1.438 ± 0.04
1.483MetVal: 1.483 ± 0.041
0.203MetTrp: 0.203 ± 0.015
0.499MetTyr: 0.499 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.645AsnAla: 3.645 ± 0.064
0.502AsnCys: 0.502 ± 0.018
2.8AsnAsp: 2.8 ± 0.066
2.955AsnGlu: 2.955 ± 0.053
1.982AsnPhe: 1.982 ± 0.041
3.447AsnGly: 3.447 ± 0.065
1.144AsnHis: 1.144 ± 0.027
3.298AsnIle: 3.298 ± 0.056
3.015AsnLys: 3.015 ± 0.06
4.504AsnLeu: 4.504 ± 0.066
0.952AsnMet: 0.952 ± 0.027
2.498AsnAsn: 2.498 ± 0.058
2.12AsnPro: 2.12 ± 0.042
2.392AsnGln: 2.392 ± 0.044
2.084AsnArg: 2.084 ± 0.041
3.252AsnSer: 3.252 ± 0.065
2.596AsnThr: 2.596 ± 0.058
3.13AsnVal: 3.13 ± 0.05
0.684AsnTrp: 0.684 ± 0.025
1.583AsnTyr: 1.583 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
2.674ProAla: 2.674 ± 0.05
0.27ProCys: 0.27 ± 0.017
2.225ProAsp: 2.225 ± 0.042
3.204ProGlu: 3.204 ± 0.047
1.683ProPhe: 1.683 ± 0.037
1.88ProGly: 1.88 ± 0.038
0.829ProHis: 0.829 ± 0.026
2.592ProIle: 2.592 ± 0.05
2.531ProLys: 2.531 ± 0.047
3.779ProLeu: 3.779 ± 0.064
0.897ProMet: 0.897 ± 0.028
1.896ProAsn: 1.896 ± 0.039
0.997ProPro: 0.997 ± 0.03
1.777ProGln: 1.777 ± 0.036
1.166ProArg: 1.166 ± 0.031
2.546ProSer: 2.546 ± 0.046
2.146ProThr: 2.146 ± 0.041
2.716ProVal: 2.716 ± 0.049
0.41ProTrp: 0.41 ± 0.018
1.111ProTyr: 1.111 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
3.836GlnAla: 3.836 ± 0.054
0.403GlnCys: 0.403 ± 0.016
2.044GlnAsp: 2.044 ± 0.042
2.331GlnGlu: 2.331 ± 0.04
1.852GlnPhe: 1.852 ± 0.034
2.704GlnGly: 2.704 ± 0.052
1.242GlnHis: 1.242 ± 0.033
2.808GlnIle: 2.808 ± 0.046
2.697GlnLys: 2.697 ± 0.047
5.364GlnLeu: 5.364 ± 0.075
1.078GlnMet: 1.078 ± 0.032
1.945GlnAsn: 1.945 ± 0.039
1.671GlnPro: 1.671 ± 0.037
3.168GlnGln: 3.168 ± 0.071
2.054GlnArg: 2.054 ± 0.039
2.996GlnSer: 2.996 ± 0.055
2.381GlnThr: 2.381 ± 0.049
3.192GlnVal: 3.192 ± 0.046
0.532GlnTrp: 0.532 ± 0.019
1.438GlnTyr: 1.438 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
2.827ArgAla: 2.827 ± 0.048
0.404ArgCys: 0.404 ± 0.017
2.173ArgAsp: 2.173 ± 0.042
2.557ArgGlu: 2.557 ± 0.047
2.038ArgPhe: 2.038 ± 0.038
2.239ArgGly: 2.239 ± 0.041
0.997ArgHis: 0.997 ± 0.029
2.844ArgIle: 2.844 ± 0.046
2.472ArgLys: 2.472 ± 0.044
4.356ArgLeu: 4.356 ± 0.056
0.98ArgMet: 0.98 ± 0.029
2.002ArgAsn: 2.002 ± 0.038
1.476ArgPro: 1.476 ± 0.036
1.904ArgGln: 1.904 ± 0.038
1.927ArgArg: 1.927 ± 0.045
2.549ArgSer: 2.549 ± 0.04
2.074ArgThr: 2.074 ± 0.041
2.844ArgVal: 2.844 ± 0.052
0.511ArgTrp: 0.511 ± 0.019
1.583ArgTyr: 1.583 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
5.338SerAla: 5.338 ± 0.07
0.72SerCys: 0.72 ± 0.027
4.057SerAsp: 4.057 ± 0.07
4.848SerGlu: 4.848 ± 0.073
3.358SerPhe: 3.358 ± 0.052
4.89SerGly: 4.89 ± 0.084
1.643SerHis: 1.643 ± 0.033
4.814SerIle: 4.814 ± 0.065
4.433SerLys: 4.433 ± 0.058
7.237SerLeu: 7.237 ± 0.078
1.716SerMet: 1.716 ± 0.043
3.418SerAsn: 3.418 ± 0.061
2.618SerPro: 2.618 ± 0.052
3.247SerGln: 3.247 ± 0.049
2.747SerArg: 2.747 ± 0.052
5.496SerSer: 5.496 ± 0.081
3.746SerThr: 3.746 ± 0.06
4.75SerVal: 4.75 ± 0.058
0.835SerTrp: 0.835 ± 0.027
2.27SerTyr: 2.27 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
4.325ThrAla: 4.325 ± 0.07
0.509ThrCys: 0.509 ± 0.019
3.052ThrAsp: 3.052 ± 0.058
3.513ThrGlu: 3.513 ± 0.052
2.305ThrPhe: 2.305 ± 0.047
3.762ThrGly: 3.762 ± 0.066
1.267ThrHis: 1.267 ± 0.035
3.41ThrIle: 3.41 ± 0.059
2.929ThrLys: 2.929 ± 0.048
5.872ThrLeu: 5.872 ± 0.077
1.098ThrMet: 1.098 ± 0.029
2.352ThrAsn: 2.352 ± 0.046
2.72ThrPro: 2.72 ± 0.051
2.629ThrGln: 2.629 ± 0.047
2.044ThrArg: 2.044 ± 0.044
3.847ThrSer: 3.847 ± 0.06
2.851ThrThr: 2.851 ± 0.057
3.771ThrVal: 3.771 ± 0.06
0.532ThrTrp: 0.532 ± 0.021
1.538ThrTyr: 1.538 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
5.486ValAla: 5.486 ± 0.086
0.725ValCys: 0.725 ± 0.025
4.005ValAsp: 4.005 ± 0.057
4.556ValGlu: 4.556 ± 0.06
2.77ValPhe: 2.77 ± 0.054
4.173ValGly: 4.173 ± 0.059
1.174ValHis: 1.174 ± 0.025
4.815ValIle: 4.815 ± 0.056
4.036ValLys: 4.036 ± 0.065
6.309ValLeu: 6.309 ± 0.08
1.681ValMet: 1.681 ± 0.04
3.411ValAsn: 3.411 ± 0.059
2.378ValPro: 2.378 ± 0.047
2.223ValGln: 2.223 ± 0.041
2.621ValArg: 2.621 ± 0.05
5.052ValSer: 5.052 ± 0.059
4.118ValThr: 4.118 ± 0.063
4.656ValVal: 4.656 ± 0.075
0.658ValTrp: 0.658 ± 0.022
1.812ValTyr: 1.812 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.668TrpAla: 0.668 ± 0.021
0.15TrpCys: 0.15 ± 0.01
0.569TrpAsp: 0.569 ± 0.021
0.508TrpGlu: 0.508 ± 0.02
0.536TrpPhe: 0.536 ± 0.023
0.701TrpGly: 0.701 ± 0.026
0.298TrpHis: 0.298 ± 0.015
0.633TrpIle: 0.633 ± 0.025
0.594TrpLys: 0.594 ± 0.022
1.477TrpLeu: 1.477 ± 0.037
0.276TrpMet: 0.276 ± 0.014
0.502TrpAsn: 0.502 ± 0.02
0.399TrpPro: 0.399 ± 0.018
0.827TrpGln: 0.827 ± 0.026
0.539TrpArg: 0.539 ± 0.021
0.715TrpSer: 0.715 ± 0.025
0.475TrpThr: 0.475 ± 0.021
0.708TrpVal: 0.708 ± 0.024
0.159TrpTrp: 0.159 ± 0.012
0.358TrpTyr: 0.358 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.117TyrAla: 2.117 ± 0.039
0.361TyrCys: 0.361 ± 0.017
1.769TyrAsp: 1.769 ± 0.048
1.62TyrGlu: 1.62 ± 0.04
1.533TyrPhe: 1.533 ± 0.038
2.024TyrGly: 2.024 ± 0.047
0.839TyrHis: 0.839 ± 0.026
1.777TyrIle: 1.777 ± 0.039
1.752TyrLys: 1.752 ± 0.039
3.347TyrLeu: 3.347 ± 0.055
0.619TyrMet: 0.619 ± 0.022
1.206TyrAsn: 1.206 ± 0.029
1.276TyrPro: 1.276 ± 0.036
1.948TyrGln: 1.948 ± 0.041
1.61TyrArg: 1.61 ± 0.036
2.359TyrSer: 2.359 ± 0.046
1.522TyrThr: 1.522 ± 0.036
1.698TyrVal: 1.698 ± 0.034
0.408TyrTrp: 0.408 ± 0.018
0.99TyrTyr: 0.99 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3793 proteins (1290292 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski