Amino acid dipepetide frequency for Serratia phage Serbin

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.127AlaAla: 8.127 ± 1.343
1.151AlaCys: 1.151 ± 0.332
4.531AlaAsp: 4.531 ± 0.681
5.897AlaGlu: 5.897 ± 0.684
2.229AlaPhe: 2.229 ± 0.343
7.192AlaGly: 7.192 ± 0.703
1.079AlaHis: 1.079 ± 0.27
4.531AlaIle: 4.531 ± 0.478
5.466AlaLys: 5.466 ± 0.694
6.904AlaLeu: 6.904 ± 0.709
2.229AlaMet: 2.229 ± 0.384
2.949AlaAsn: 2.949 ± 0.556
2.445AlaPro: 2.445 ± 0.438
3.524AlaGln: 3.524 ± 0.672
3.955AlaArg: 3.955 ± 0.583
4.962AlaSer: 4.962 ± 0.663
4.962AlaThr: 4.962 ± 0.65
4.459AlaVal: 4.459 ± 0.537
0.791AlaTrp: 0.791 ± 0.198
2.517AlaTyr: 2.517 ± 0.384
0.0AlaXaa: 0.0 ± 0.0
Cys
0.719CysAla: 0.719 ± 0.218
0.288CysCys: 0.288 ± 0.146
0.431CysAsp: 0.431 ± 0.177
0.575CysGlu: 0.575 ± 0.167
0.216CysPhe: 0.216 ± 0.116
1.151CysGly: 1.151 ± 0.28
0.072CysHis: 0.072 ± 0.068
0.647CysIle: 0.647 ± 0.198
0.719CysLys: 0.719 ± 0.23
1.079CysLeu: 1.079 ± 0.268
0.216CysMet: 0.216 ± 0.133
0.36CysAsn: 0.36 ± 0.165
0.288CysPro: 0.288 ± 0.119
0.288CysGln: 0.288 ± 0.142
0.863CysArg: 0.863 ± 0.308
0.791CysSer: 0.791 ± 0.245
0.719CysThr: 0.719 ± 0.261
0.863CysVal: 0.863 ± 0.237
0.072CysTrp: 0.072 ± 0.058
0.431CysTyr: 0.431 ± 0.184
0.0CysXaa: 0.0 ± 0.0
Asp
4.315AspAla: 4.315 ± 0.638
0.647AspCys: 0.647 ± 0.185
3.236AspAsp: 3.236 ± 0.53
4.459AspGlu: 4.459 ± 0.622
2.301AspPhe: 2.301 ± 0.392
5.394AspGly: 5.394 ± 0.71
0.791AspHis: 0.791 ± 0.246
3.74AspIle: 3.74 ± 0.511
2.805AspLys: 2.805 ± 0.401
4.459AspLeu: 4.459 ± 0.577
1.942AspMet: 1.942 ± 0.383
2.733AspAsn: 2.733 ± 0.444
2.805AspPro: 2.805 ± 0.449
2.229AspGln: 2.229 ± 0.38
2.589AspArg: 2.589 ± 0.325
3.164AspSer: 3.164 ± 0.461
3.02AspThr: 3.02 ± 0.567
3.524AspVal: 3.524 ± 0.565
1.079AspTrp: 1.079 ± 0.286
2.445AspTyr: 2.445 ± 0.431
0.0AspXaa: 0.0 ± 0.0
Glu
5.178GluAla: 5.178 ± 0.763
0.36GluCys: 0.36 ± 0.156
4.099GluAsp: 4.099 ± 0.46
7.551GluGlu: 7.551 ± 1.729
2.877GluPhe: 2.877 ± 0.441
4.243GluGly: 4.243 ± 0.493
1.007GluHis: 1.007 ± 0.216
4.027GluIle: 4.027 ± 0.53
3.74GluLys: 3.74 ± 0.601
5.681GluLeu: 5.681 ± 0.962
2.589GluMet: 2.589 ± 0.5
3.164GluAsn: 3.164 ± 0.443
1.798GluPro: 1.798 ± 0.359
2.589GluGln: 2.589 ± 0.401
3.668GluArg: 3.668 ± 0.542
4.746GluSer: 4.746 ± 0.559
3.74GluThr: 3.74 ± 0.451
4.89GluVal: 4.89 ± 0.611
1.654GluTrp: 1.654 ± 0.325
2.517GluTyr: 2.517 ± 0.397
0.0GluXaa: 0.0 ± 0.0
Phe
1.798PheAla: 1.798 ± 0.389
0.719PheCys: 0.719 ± 0.247
2.589PheAsp: 2.589 ± 0.483
2.373PheGlu: 2.373 ± 0.323
0.863PhePhe: 0.863 ± 0.279
2.301PheGly: 2.301 ± 0.431
0.575PheHis: 0.575 ± 0.199
1.798PheIle: 1.798 ± 0.334
2.229PheLys: 2.229 ± 0.412
3.092PheLeu: 3.092 ± 0.441
0.288PheMet: 0.288 ± 0.116
1.726PheAsn: 1.726 ± 0.317
1.51PhePro: 1.51 ± 0.302
1.007PheGln: 1.007 ± 0.311
2.445PheArg: 2.445 ± 0.353
1.654PheSer: 1.654 ± 0.318
2.229PheThr: 2.229 ± 0.526
1.942PheVal: 1.942 ± 0.313
0.575PheTrp: 0.575 ± 0.199
1.366PheTyr: 1.366 ± 0.301
0.0PheXaa: 0.0 ± 0.0
Gly
5.25GlyAla: 5.25 ± 0.549
0.216GlyCys: 0.216 ± 0.129
4.531GlyAsp: 4.531 ± 0.517
5.106GlyGlu: 5.106 ± 0.638
2.301GlyPhe: 2.301 ± 0.355
5.25GlyGly: 5.25 ± 0.72
1.438GlyHis: 1.438 ± 0.352
4.603GlyIle: 4.603 ± 0.636
4.818GlyLys: 4.818 ± 0.558
7.048GlyLeu: 7.048 ± 0.618
2.373GlyMet: 2.373 ± 0.421
2.445GlyAsn: 2.445 ± 0.417
2.157GlyPro: 2.157 ± 0.304
4.171GlyGln: 4.171 ± 0.634
4.818GlyArg: 4.818 ± 0.57
5.466GlySer: 5.466 ± 0.678
5.178GlyThr: 5.178 ± 0.749
5.106GlyVal: 5.106 ± 0.61
1.942GlyTrp: 1.942 ± 0.4
3.955GlyTyr: 3.955 ± 0.526
0.0GlyXaa: 0.0 ± 0.0
His
0.791HisAla: 0.791 ± 0.286
0.216HisCys: 0.216 ± 0.12
1.007HisAsp: 1.007 ± 0.273
1.294HisGlu: 1.294 ± 0.224
1.079HisPhe: 1.079 ± 0.276
1.079HisGly: 1.079 ± 0.252
0.36HisHis: 0.36 ± 0.23
1.366HisIle: 1.366 ± 0.31
0.863HisLys: 0.863 ± 0.22
1.582HisLeu: 1.582 ± 0.281
0.647HisMet: 0.647 ± 0.237
0.791HisAsn: 0.791 ± 0.252
0.503HisPro: 0.503 ± 0.191
0.575HisGln: 0.575 ± 0.153
0.647HisArg: 0.647 ± 0.237
0.935HisSer: 0.935 ± 0.301
0.863HisThr: 0.863 ± 0.241
0.935HisVal: 0.935 ± 0.249
0.719HisTrp: 0.719 ± 0.21
1.007HisTyr: 1.007 ± 0.256
0.0HisXaa: 0.0 ± 0.0
Ile
3.955IleAla: 3.955 ± 0.551
0.503IleCys: 0.503 ± 0.19
2.949IleAsp: 2.949 ± 0.475
2.949IleGlu: 2.949 ± 0.355
1.366IlePhe: 1.366 ± 0.33
4.315IleGly: 4.315 ± 0.523
1.366IleHis: 1.366 ± 0.349
3.452IleIle: 3.452 ± 0.592
4.027IleLys: 4.027 ± 0.594
4.603IleLeu: 4.603 ± 0.563
1.51IleMet: 1.51 ± 0.302
2.661IleAsn: 2.661 ± 0.36
2.445IlePro: 2.445 ± 0.365
1.942IleGln: 1.942 ± 0.298
3.955IleArg: 3.955 ± 0.515
3.236IleSer: 3.236 ± 0.439
3.38IleThr: 3.38 ± 0.665
2.517IleVal: 2.517 ± 0.435
0.36IleTrp: 0.36 ± 0.162
2.086IleTyr: 2.086 ± 0.481
0.0IleXaa: 0.0 ± 0.0
Lys
6.832LysAla: 6.832 ± 0.913
0.719LysCys: 0.719 ± 0.27
3.236LysAsp: 3.236 ± 0.511
4.675LysGlu: 4.675 ± 0.713
2.445LysPhe: 2.445 ± 0.423
3.883LysGly: 3.883 ± 0.548
0.791LysHis: 0.791 ± 0.207
2.373LysIle: 2.373 ± 0.385
4.675LysLys: 4.675 ± 0.712
4.603LysLeu: 4.603 ± 0.629
1.942LysMet: 1.942 ± 0.327
2.733LysAsn: 2.733 ± 0.496
3.524LysPro: 3.524 ± 0.563
2.517LysGln: 2.517 ± 0.444
3.74LysArg: 3.74 ± 0.539
3.74LysSer: 3.74 ± 0.613
5.322LysThr: 5.322 ± 0.769
3.452LysVal: 3.452 ± 0.494
0.863LysTrp: 0.863 ± 0.194
2.733LysTyr: 2.733 ± 0.45
0.0LysXaa: 0.0 ± 0.0
Leu
5.825LeuAla: 5.825 ± 0.691
1.079LeuCys: 1.079 ± 0.27
5.322LeuAsp: 5.322 ± 0.526
5.969LeuGlu: 5.969 ± 0.683
2.445LeuPhe: 2.445 ± 0.482
6.113LeuGly: 6.113 ± 0.771
1.726LeuHis: 1.726 ± 0.43
3.668LeuIle: 3.668 ± 0.445
5.969LeuLys: 5.969 ± 0.721
6.113LeuLeu: 6.113 ± 0.774
3.236LeuMet: 3.236 ± 0.406
4.099LeuAsn: 4.099 ± 0.45
2.805LeuPro: 2.805 ± 0.345
3.668LeuGln: 3.668 ± 0.632
6.329LeuArg: 6.329 ± 0.687
5.969LeuSer: 5.969 ± 0.495
5.897LeuThr: 5.897 ± 0.604
5.825LeuVal: 5.825 ± 0.613
1.294LeuTrp: 1.294 ± 0.342
3.092LeuTyr: 3.092 ± 0.486
0.0LeuXaa: 0.0 ± 0.0
Met
3.596MetAla: 3.596 ± 0.461
0.288MetCys: 0.288 ± 0.147
1.438MetAsp: 1.438 ± 0.368
1.87MetGlu: 1.87 ± 0.366
1.294MetPhe: 1.294 ± 0.291
2.229MetGly: 2.229 ± 0.406
0.647MetHis: 0.647 ± 0.16
1.798MetIle: 1.798 ± 0.413
2.661MetLys: 2.661 ± 0.394
1.726MetLeu: 1.726 ± 0.356
0.503MetMet: 0.503 ± 0.206
1.223MetAsn: 1.223 ± 0.321
1.151MetPro: 1.151 ± 0.293
1.223MetGln: 1.223 ± 0.299
1.223MetArg: 1.223 ± 0.291
1.798MetSer: 1.798 ± 0.317
1.798MetThr: 1.798 ± 0.274
1.366MetVal: 1.366 ± 0.268
0.288MetTrp: 0.288 ± 0.113
1.007MetTyr: 1.007 ± 0.22
0.0MetXaa: 0.0 ± 0.0
Asn
2.805AsnAla: 2.805 ± 0.587
0.288AsnCys: 0.288 ± 0.125
1.798AsnAsp: 1.798 ± 0.315
2.733AsnGlu: 2.733 ± 0.479
1.87AsnPhe: 1.87 ± 0.328
3.955AsnGly: 3.955 ± 0.598
0.503AsnHis: 0.503 ± 0.173
2.301AsnIle: 2.301 ± 0.509
2.229AsnLys: 2.229 ± 0.38
4.315AsnLeu: 4.315 ± 0.6
0.935AsnMet: 0.935 ± 0.27
1.726AsnAsn: 1.726 ± 0.387
2.086AsnPro: 2.086 ± 0.472
2.014AsnGln: 2.014 ± 0.316
2.157AsnArg: 2.157 ± 0.386
2.014AsnSer: 2.014 ± 0.389
1.798AsnThr: 1.798 ± 0.372
2.877AsnVal: 2.877 ± 0.352
1.151AsnTrp: 1.151 ± 0.255
1.87AsnTyr: 1.87 ± 0.376
0.0AsnXaa: 0.0 ± 0.0
Pro
3.74ProAla: 3.74 ± 0.49
0.431ProCys: 0.431 ± 0.216
3.092ProAsp: 3.092 ± 0.555
3.668ProGlu: 3.668 ± 0.401
1.151ProPhe: 1.151 ± 0.261
3.38ProGly: 3.38 ± 0.584
1.007ProHis: 1.007 ± 0.295
1.582ProIle: 1.582 ± 0.346
2.014ProLys: 2.014 ± 0.421
2.445ProLeu: 2.445 ± 0.397
0.647ProMet: 0.647 ± 0.197
2.014ProAsn: 2.014 ± 0.372
1.007ProPro: 1.007 ± 0.216
1.223ProGln: 1.223 ± 0.22
1.223ProArg: 1.223 ± 0.285
2.086ProSer: 2.086 ± 0.331
2.949ProThr: 2.949 ± 0.424
2.949ProVal: 2.949 ± 0.492
0.647ProTrp: 0.647 ± 0.262
1.007ProTyr: 1.007 ± 0.237
0.0ProXaa: 0.0 ± 0.0
Gln
4.675GlnAla: 4.675 ± 0.779
0.36GlnCys: 0.36 ± 0.149
2.373GlnAsp: 2.373 ± 0.46
2.661GlnGlu: 2.661 ± 0.366
1.079GlnPhe: 1.079 ± 0.274
3.02GlnGly: 3.02 ± 0.48
0.575GlnHis: 0.575 ± 0.207
2.157GlnIle: 2.157 ± 0.367
2.589GlnLys: 2.589 ± 0.482
4.603GlnLeu: 4.603 ± 0.559
1.438GlnMet: 1.438 ± 0.267
1.51GlnAsn: 1.51 ± 0.331
1.151GlnPro: 1.151 ± 0.297
1.438GlnGln: 1.438 ± 0.323
2.229GlnArg: 2.229 ± 0.385
1.654GlnSer: 1.654 ± 0.357
2.301GlnThr: 2.301 ± 0.341
3.02GlnVal: 3.02 ± 0.499
0.575GlnTrp: 0.575 ± 0.19
1.942GlnTyr: 1.942 ± 0.37
0.0GlnXaa: 0.0 ± 0.0
Arg
4.243ArgAla: 4.243 ± 0.574
0.431ArgCys: 0.431 ± 0.157
3.452ArgAsp: 3.452 ± 0.53
3.452ArgGlu: 3.452 ± 0.513
1.726ArgPhe: 1.726 ± 0.348
3.452ArgGly: 3.452 ± 0.506
1.294ArgHis: 1.294 ± 0.291
3.883ArgIle: 3.883 ± 0.471
4.603ArgLys: 4.603 ± 0.645
5.466ArgLeu: 5.466 ± 0.537
1.798ArgMet: 1.798 ± 0.435
2.157ArgAsn: 2.157 ± 0.418
1.726ArgPro: 1.726 ± 0.377
2.661ArgGln: 2.661 ± 0.372
3.668ArgArg: 3.668 ± 0.558
4.027ArgSer: 4.027 ± 0.545
3.02ArgThr: 3.02 ± 0.613
3.812ArgVal: 3.812 ± 0.42
1.007ArgTrp: 1.007 ± 0.246
1.654ArgTyr: 1.654 ± 0.389
0.0ArgXaa: 0.0 ± 0.0
Ser
4.675SerAla: 4.675 ± 0.511
0.647SerCys: 0.647 ± 0.282
3.02SerAsp: 3.02 ± 0.414
2.517SerGlu: 2.517 ± 0.546
2.877SerPhe: 2.877 ± 0.409
6.329SerGly: 6.329 ± 0.675
0.719SerHis: 0.719 ± 0.203
2.949SerIle: 2.949 ± 0.431
3.668SerLys: 3.668 ± 0.429
5.681SerLeu: 5.681 ± 0.566
1.654SerMet: 1.654 ± 0.319
1.582SerAsn: 1.582 ± 0.33
2.229SerPro: 2.229 ± 0.502
2.949SerGln: 2.949 ± 0.443
3.236SerArg: 3.236 ± 0.455
3.74SerSer: 3.74 ± 0.52
3.812SerThr: 3.812 ± 0.431
3.955SerVal: 3.955 ± 0.563
1.366SerTrp: 1.366 ± 0.342
1.87SerTyr: 1.87 ± 0.371
0.0SerXaa: 0.0 ± 0.0
Thr
4.89ThrAla: 4.89 ± 0.68
1.007ThrCys: 1.007 ± 0.339
3.668ThrAsp: 3.668 ± 0.471
5.25ThrGlu: 5.25 ± 0.638
1.798ThrPhe: 1.798 ± 0.345
6.185ThrGly: 6.185 ± 0.668
1.366ThrHis: 1.366 ± 0.293
3.092ThrIle: 3.092 ± 0.471
3.74ThrLys: 3.74 ± 0.496
6.401ThrLeu: 6.401 ± 0.936
1.366ThrMet: 1.366 ± 0.34
1.582ThrAsn: 1.582 ± 0.327
3.092ThrPro: 3.092 ± 0.39
2.014ThrGln: 2.014 ± 0.416
3.596ThrArg: 3.596 ± 0.563
3.092ThrSer: 3.092 ± 0.464
3.524ThrThr: 3.524 ± 0.654
3.524ThrVal: 3.524 ± 0.457
0.791ThrTrp: 0.791 ± 0.189
2.014ThrTyr: 2.014 ± 0.389
0.0ThrXaa: 0.0 ± 0.0
Val
4.603ValAla: 4.603 ± 0.7
0.503ValCys: 0.503 ± 0.151
3.524ValAsp: 3.524 ± 0.508
4.099ValGlu: 4.099 ± 0.63
1.438ValPhe: 1.438 ± 0.326
4.171ValGly: 4.171 ± 0.611
1.079ValHis: 1.079 ± 0.299
2.949ValIle: 2.949 ± 0.604
4.603ValLys: 4.603 ± 0.509
5.969ValLeu: 5.969 ± 0.582
2.589ValMet: 2.589 ± 0.414
3.164ValAsn: 3.164 ± 0.437
2.877ValPro: 2.877 ± 0.452
2.445ValGln: 2.445 ± 0.469
3.524ValArg: 3.524 ± 0.574
3.524ValSer: 3.524 ± 0.501
4.459ValThr: 4.459 ± 0.447
4.531ValVal: 4.531 ± 0.668
1.51ValTrp: 1.51 ± 0.291
2.014ValTyr: 2.014 ± 0.297
0.0ValXaa: 0.0 ± 0.0
Trp
1.079TrpAla: 1.079 ± 0.316
0.431TrpCys: 0.431 ± 0.197
1.007TrpAsp: 1.007 ± 0.256
1.223TrpGlu: 1.223 ± 0.294
0.431TrpPhe: 0.431 ± 0.21
1.582TrpGly: 1.582 ± 0.454
0.288TrpHis: 0.288 ± 0.128
0.791TrpIle: 0.791 ± 0.21
1.51TrpLys: 1.51 ± 0.304
1.726TrpLeu: 1.726 ± 0.284
0.216TrpMet: 0.216 ± 0.151
1.223TrpAsn: 1.223 ± 0.242
0.863TrpPro: 0.863 ± 0.313
0.575TrpGln: 0.575 ± 0.161
0.719TrpArg: 0.719 ± 0.224
1.223TrpSer: 1.223 ± 0.285
0.719TrpThr: 0.719 ± 0.199
1.798TrpVal: 1.798 ± 0.344
0.144TrpTrp: 0.144 ± 0.099
0.36TrpTyr: 0.36 ± 0.193
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.805TyrAla: 2.805 ± 0.402
0.503TyrCys: 0.503 ± 0.207
2.373TyrAsp: 2.373 ± 0.368
1.798TyrGlu: 1.798 ± 0.299
1.294TyrPhe: 1.294 ± 0.289
2.949TyrGly: 2.949 ± 0.441
0.575TyrHis: 0.575 ± 0.207
1.87TyrIle: 1.87 ± 0.384
1.942TyrLys: 1.942 ± 0.302
2.949TyrLeu: 2.949 ± 0.447
0.935TyrMet: 0.935 ± 0.341
1.582TyrAsn: 1.582 ± 0.308
1.798TyrPro: 1.798 ± 0.38
2.301TyrGln: 2.301 ± 0.402
2.949TyrArg: 2.949 ± 0.55
1.654TyrSer: 1.654 ± 0.339
2.301TyrThr: 2.301 ± 0.531
2.229TyrVal: 2.229 ± 0.344
1.079TyrTrp: 1.079 ± 0.294
1.654TyrTyr: 1.654 ± 0.36
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (13906 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski