Amino acid dipepetide frequency for Bacillus virus IEBH

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.566AlaAla: 4.566 ± 0.813
0.86AlaCys: 0.86 ± 0.24
3.176AlaAsp: 3.176 ± 0.51
4.699AlaGlu: 4.699 ± 0.466
2.779AlaPhe: 2.779 ± 0.399
3.243AlaGly: 3.243 ± 0.466
0.86AlaHis: 0.86 ± 0.241
4.765AlaIle: 4.765 ± 0.512
5.89AlaLys: 5.89 ± 0.627
6.684AlaLeu: 6.684 ± 1.264
2.515AlaMet: 2.515 ± 0.588
3.11AlaAsn: 3.11 ± 0.415
1.588AlaPro: 1.588 ± 0.257
3.11AlaGln: 3.11 ± 0.491
2.25AlaArg: 2.25 ± 0.413
3.176AlaSer: 3.176 ± 0.545
4.037AlaThr: 4.037 ± 0.628
4.368AlaVal: 4.368 ± 0.522
0.728AlaTrp: 0.728 ± 0.236
1.919AlaTyr: 1.919 ± 0.338
0.0AlaXaa: 0.0 ± 0.0
Cys
0.397CysAla: 0.397 ± 0.168
0.199CysCys: 0.199 ± 0.107
0.993CysAsp: 0.993 ± 0.3
1.125CysGlu: 1.125 ± 0.262
0.397CysPhe: 0.397 ± 0.167
0.596CysGly: 0.596 ± 0.215
0.0CysHis: 0.0 ± 0.0
0.199CysIle: 0.199 ± 0.109
0.397CysLys: 0.397 ± 0.16
0.331CysLeu: 0.331 ± 0.151
0.132CysMet: 0.132 ± 0.106
0.463CysAsn: 0.463 ± 0.213
0.662CysPro: 0.662 ± 0.246
0.199CysGln: 0.199 ± 0.129
0.199CysArg: 0.199 ± 0.11
0.463CysSer: 0.463 ± 0.186
0.529CysThr: 0.529 ± 0.253
0.463CysVal: 0.463 ± 0.183
0.0CysTrp: 0.0 ± 0.0
0.265CysTyr: 0.265 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
3.574AspAla: 3.574 ± 0.474
0.529AspCys: 0.529 ± 0.227
2.978AspAsp: 2.978 ± 0.486
4.765AspGlu: 4.765 ± 0.737
3.044AspPhe: 3.044 ± 0.495
3.971AspGly: 3.971 ± 0.709
0.794AspHis: 0.794 ± 0.254
4.566AspIle: 4.566 ± 0.569
4.566AspLys: 4.566 ± 0.613
4.302AspLeu: 4.302 ± 0.572
1.985AspMet: 1.985 ± 0.353
2.713AspAsn: 2.713 ± 0.402
1.456AspPro: 1.456 ± 0.335
1.853AspGln: 1.853 ± 0.421
2.25AspArg: 2.25 ± 0.385
2.515AspSer: 2.515 ± 0.41
2.449AspThr: 2.449 ± 0.429
5.162AspVal: 5.162 ± 0.612
0.993AspTrp: 0.993 ± 0.279
2.581AspTyr: 2.581 ± 0.451
0.0AspXaa: 0.0 ± 0.0
Glu
6.022GluAla: 6.022 ± 0.694
0.926GluCys: 0.926 ± 0.291
3.044GluAsp: 3.044 ± 0.533
8.074GluGlu: 8.074 ± 1.04
3.044GluPhe: 3.044 ± 0.371
3.309GluGly: 3.309 ± 0.47
1.588GluHis: 1.588 ± 0.425
6.684GluIle: 6.684 ± 0.901
7.412GluLys: 7.412 ± 0.927
7.412GluLeu: 7.412 ± 0.71
2.779GluMet: 2.779 ± 0.567
3.971GluAsn: 3.971 ± 0.66
2.316GluPro: 2.316 ± 0.655
3.309GluGln: 3.309 ± 0.738
3.838GluArg: 3.838 ± 0.603
4.169GluSer: 4.169 ± 0.424
3.706GluThr: 3.706 ± 0.499
5.691GluVal: 5.691 ± 0.666
1.39GluTrp: 1.39 ± 0.271
2.581GluTyr: 2.581 ± 0.545
0.0GluXaa: 0.0 ± 0.0
Phe
2.382PheAla: 2.382 ± 0.423
0.265PheCys: 0.265 ± 0.134
3.11PheAsp: 3.11 ± 0.455
3.11PheGlu: 3.11 ± 0.561
1.324PhePhe: 1.324 ± 0.365
2.184PheGly: 2.184 ± 0.332
0.596PheHis: 0.596 ± 0.22
3.309PheIle: 3.309 ± 0.528
2.978PheLys: 2.978 ± 0.447
2.647PheLeu: 2.647 ± 0.445
1.456PheMet: 1.456 ± 0.318
1.919PheAsn: 1.919 ± 0.353
1.324PhePro: 1.324 ± 0.292
1.191PheGln: 1.191 ± 0.209
1.853PheArg: 1.853 ± 0.447
1.985PheSer: 1.985 ± 0.321
2.779PheThr: 2.779 ± 0.419
2.382PheVal: 2.382 ± 0.396
0.265PheTrp: 0.265 ± 0.136
2.118PheTyr: 2.118 ± 0.383
0.0PheXaa: 0.0 ± 0.0
Gly
2.713GlyAla: 2.713 ± 0.493
0.397GlyCys: 0.397 ± 0.174
3.044GlyAsp: 3.044 ± 0.501
4.169GlyGlu: 4.169 ± 0.644
2.978GlyPhe: 2.978 ± 0.465
3.574GlyGly: 3.574 ± 0.462
0.662GlyHis: 0.662 ± 0.195
5.294GlyIle: 5.294 ± 0.799
5.625GlyLys: 5.625 ± 0.557
3.971GlyLeu: 3.971 ± 0.681
2.779GlyMet: 2.779 ± 0.621
3.838GlyAsn: 3.838 ± 0.55
0.728GlyPro: 0.728 ± 0.221
1.456GlyGln: 1.456 ± 0.343
3.243GlyArg: 3.243 ± 0.448
2.846GlySer: 2.846 ± 0.475
3.309GlyThr: 3.309 ± 0.581
3.243GlyVal: 3.243 ± 0.346
0.794GlyTrp: 0.794 ± 0.235
3.441GlyTyr: 3.441 ± 0.491
0.0GlyXaa: 0.0 ± 0.0
His
0.662HisAla: 0.662 ± 0.256
0.199HisCys: 0.199 ± 0.117
1.191HisAsp: 1.191 ± 0.301
1.059HisGlu: 1.059 ± 0.315
0.926HisPhe: 0.926 ± 0.232
0.926HisGly: 0.926 ± 0.237
0.397HisHis: 0.397 ± 0.182
1.39HisIle: 1.39 ± 0.34
1.588HisLys: 1.588 ± 0.327
1.853HisLeu: 1.853 ± 0.381
0.265HisMet: 0.265 ± 0.131
0.926HisAsn: 0.926 ± 0.33
0.529HisPro: 0.529 ± 0.194
0.662HisGln: 0.662 ± 0.213
0.926HisArg: 0.926 ± 0.256
1.059HisSer: 1.059 ± 0.271
0.993HisThr: 0.993 ± 0.292
0.728HisVal: 0.728 ± 0.235
0.265HisTrp: 0.265 ± 0.12
0.596HisTyr: 0.596 ± 0.19
0.0HisXaa: 0.0 ± 0.0
Ile
6.088IleAla: 6.088 ± 0.58
0.265IleCys: 0.265 ± 0.132
4.963IleAsp: 4.963 ± 0.632
6.154IleGlu: 6.154 ± 0.686
1.39IlePhe: 1.39 ± 0.366
3.838IleGly: 3.838 ± 0.618
1.257IleHis: 1.257 ± 0.308
4.897IleIle: 4.897 ± 0.603
7.015IleLys: 7.015 ± 0.675
5.096IleLeu: 5.096 ± 0.646
1.721IleMet: 1.721 ± 0.295
4.963IleAsn: 4.963 ± 0.697
2.449IlePro: 2.449 ± 0.433
2.647IleGln: 2.647 ± 0.531
3.507IleArg: 3.507 ± 0.414
4.632IleSer: 4.632 ± 0.598
4.632IleThr: 4.632 ± 0.686
4.897IleVal: 4.897 ± 0.651
0.728IleTrp: 0.728 ± 0.212
2.051IleTyr: 2.051 ± 0.382
0.0IleXaa: 0.0 ± 0.0
Lys
5.427LysAla: 5.427 ± 0.507
0.463LysCys: 0.463 ± 0.168
6.221LysAsp: 6.221 ± 0.709
8.802LysGlu: 8.802 ± 1.246
2.779LysPhe: 2.779 ± 0.459
4.103LysGly: 4.103 ± 0.618
1.39LysHis: 1.39 ± 0.31
5.691LysIle: 5.691 ± 0.742
8.338LysLys: 8.338 ± 1.072
7.213LysLeu: 7.213 ± 0.581
2.978LysMet: 2.978 ± 0.472
4.235LysAsn: 4.235 ± 0.455
2.713LysPro: 2.713 ± 0.406
4.699LysGln: 4.699 ± 0.528
5.36LysArg: 5.36 ± 0.65
4.5LysSer: 4.5 ± 0.507
5.956LysThr: 5.956 ± 0.548
5.029LysVal: 5.029 ± 0.575
1.456LysTrp: 1.456 ± 0.289
4.037LysTyr: 4.037 ± 0.739
0.0LysXaa: 0.0 ± 0.0
Leu
5.294LeuAla: 5.294 ± 0.697
0.463LeuCys: 0.463 ± 0.155
5.029LeuAsp: 5.029 ± 0.528
5.493LeuGlu: 5.493 ± 0.545
2.912LeuPhe: 2.912 ± 0.399
5.029LeuGly: 5.029 ± 0.605
1.39LeuHis: 1.39 ± 0.328
4.897LeuIle: 4.897 ± 0.632
8.603LeuLys: 8.603 ± 0.789
5.89LeuLeu: 5.89 ± 0.635
2.118LeuMet: 2.118 ± 0.435
4.699LeuAsn: 4.699 ± 0.645
3.11LeuPro: 3.11 ± 0.735
2.912LeuGln: 2.912 ± 0.426
3.243LeuArg: 3.243 ± 0.416
5.029LeuSer: 5.029 ± 0.665
4.434LeuThr: 4.434 ± 0.776
4.566LeuVal: 4.566 ± 0.592
0.463LeuTrp: 0.463 ± 0.183
3.176LeuTyr: 3.176 ± 0.491
0.0LeuXaa: 0.0 ± 0.0
Met
2.449MetAla: 2.449 ± 0.56
0.397MetCys: 0.397 ± 0.181
1.721MetAsp: 1.721 ± 0.285
2.978MetGlu: 2.978 ± 0.519
0.993MetPhe: 0.993 ± 0.233
1.588MetGly: 1.588 ± 0.485
0.794MetHis: 0.794 ± 0.238
2.184MetIle: 2.184 ± 0.376
3.706MetLys: 3.706 ± 0.409
2.515MetLeu: 2.515 ± 0.539
1.456MetMet: 1.456 ± 0.34
2.515MetAsn: 2.515 ± 0.468
0.662MetPro: 0.662 ± 0.295
1.191MetGln: 1.191 ± 0.241
1.787MetArg: 1.787 ± 0.424
2.051MetSer: 2.051 ± 0.389
1.654MetThr: 1.654 ± 0.312
1.125MetVal: 1.125 ± 0.277
0.132MetTrp: 0.132 ± 0.085
1.191MetTyr: 1.191 ± 0.333
0.0MetXaa: 0.0 ± 0.0
Asn
2.912AsnAla: 2.912 ± 0.638
0.596AsnCys: 0.596 ± 0.222
3.772AsnAsp: 3.772 ± 0.502
4.103AsnGlu: 4.103 ± 0.5
1.919AsnPhe: 1.919 ± 0.253
5.162AsnGly: 5.162 ± 0.646
1.059AsnHis: 1.059 ± 0.282
4.169AsnIle: 4.169 ± 0.585
5.228AsnLys: 5.228 ± 0.782
4.235AsnLeu: 4.235 ± 0.532
2.051AsnMet: 2.051 ± 0.317
3.176AsnAsn: 3.176 ± 0.443
2.647AsnPro: 2.647 ± 0.378
2.449AsnGln: 2.449 ± 0.335
1.985AsnArg: 1.985 ± 0.404
3.044AsnSer: 3.044 ± 0.633
2.713AsnThr: 2.713 ± 0.474
3.904AsnVal: 3.904 ± 0.586
0.463AsnTrp: 0.463 ± 0.194
1.257AsnTyr: 1.257 ± 0.331
0.066AsnXaa: 0.066 ± 0.067
Pro
1.324ProAla: 1.324 ± 0.324
0.331ProCys: 0.331 ± 0.148
1.787ProAsp: 1.787 ± 0.473
1.919ProGlu: 1.919 ± 0.428
1.257ProPhe: 1.257 ± 0.307
1.191ProGly: 1.191 ± 0.303
0.529ProHis: 0.529 ± 0.19
1.588ProIle: 1.588 ± 0.351
2.184ProLys: 2.184 ± 0.46
2.118ProLeu: 2.118 ± 0.346
1.059ProMet: 1.059 ± 0.238
1.919ProAsn: 1.919 ± 0.401
1.059ProPro: 1.059 ± 0.278
1.721ProGln: 1.721 ± 0.403
0.993ProArg: 0.993 ± 0.288
2.713ProSer: 2.713 ± 0.356
2.647ProThr: 2.647 ± 0.406
2.449ProVal: 2.449 ± 0.507
0.199ProTrp: 0.199 ± 0.111
1.456ProTyr: 1.456 ± 0.315
0.0ProXaa: 0.0 ± 0.0
Gln
2.515GlnAla: 2.515 ± 0.602
0.132GlnCys: 0.132 ± 0.087
1.721GlnAsp: 1.721 ± 0.307
2.779GlnGlu: 2.779 ± 0.609
1.191GlnPhe: 1.191 ± 0.288
2.581GlnGly: 2.581 ± 0.449
0.662GlnHis: 0.662 ± 0.217
3.64GlnIle: 3.64 ± 0.536
3.11GlnLys: 3.11 ± 0.464
3.971GlnLeu: 3.971 ± 0.495
1.39GlnMet: 1.39 ± 0.293
1.721GlnAsn: 1.721 ± 0.281
1.059GlnPro: 1.059 ± 0.223
2.316GlnGln: 2.316 ± 0.641
2.449GlnArg: 2.449 ± 0.594
1.853GlnSer: 1.853 ± 0.333
2.713GlnThr: 2.713 ± 0.339
1.853GlnVal: 1.853 ± 0.368
0.596GlnTrp: 0.596 ± 0.197
1.588GlnTyr: 1.588 ± 0.293
0.0GlnXaa: 0.0 ± 0.0
Arg
2.382ArgAla: 2.382 ± 0.438
0.331ArgCys: 0.331 ± 0.145
2.382ArgAsp: 2.382 ± 0.451
3.243ArgGlu: 3.243 ± 0.564
1.654ArgPhe: 1.654 ± 0.322
2.449ArgGly: 2.449 ± 0.508
1.059ArgHis: 1.059 ± 0.252
3.838ArgIle: 3.838 ± 0.596
5.493ArgLys: 5.493 ± 1.014
3.772ArgLeu: 3.772 ± 0.499
1.39ArgMet: 1.39 ± 0.281
2.581ArgAsn: 2.581 ± 0.459
1.257ArgPro: 1.257 ± 0.36
2.051ArgGln: 2.051 ± 0.483
2.581ArgArg: 2.581 ± 0.416
2.051ArgSer: 2.051 ± 0.378
1.787ArgThr: 1.787 ± 0.416
3.176ArgVal: 3.176 ± 0.441
0.662ArgTrp: 0.662 ± 0.221
2.051ArgTyr: 2.051 ± 0.352
0.0ArgXaa: 0.0 ± 0.0
Ser
3.375SerAla: 3.375 ± 0.577
0.331SerCys: 0.331 ± 0.151
2.779SerAsp: 2.779 ± 0.33
4.434SerGlu: 4.434 ± 0.722
2.713SerPhe: 2.713 ± 0.376
3.441SerGly: 3.441 ± 0.599
1.059SerHis: 1.059 ± 0.273
4.037SerIle: 4.037 ± 0.497
5.228SerLys: 5.228 ± 0.636
4.235SerLeu: 4.235 ± 0.616
1.588SerMet: 1.588 ± 0.339
3.507SerAsn: 3.507 ± 0.62
1.588SerPro: 1.588 ± 0.268
2.316SerGln: 2.316 ± 0.445
2.449SerArg: 2.449 ± 0.438
2.978SerSer: 2.978 ± 0.619
3.11SerThr: 3.11 ± 0.438
3.044SerVal: 3.044 ± 0.589
0.662SerTrp: 0.662 ± 0.209
1.853SerTyr: 1.853 ± 0.283
0.066SerXaa: 0.066 ± 0.067
Thr
5.162ThrAla: 5.162 ± 0.723
0.331ThrCys: 0.331 ± 0.148
2.25ThrAsp: 2.25 ± 0.417
4.103ThrGlu: 4.103 ± 0.547
2.912ThrPhe: 2.912 ± 0.476
4.434ThrGly: 4.434 ± 0.657
1.059ThrHis: 1.059 ± 0.205
3.772ThrIle: 3.772 ± 0.749
4.302ThrLys: 4.302 ± 0.509
4.765ThrLeu: 4.765 ± 0.78
1.324ThrMet: 1.324 ± 0.355
4.235ThrAsn: 4.235 ± 0.55
2.779ThrPro: 2.779 ± 0.576
1.588ThrGln: 1.588 ± 0.366
1.721ThrArg: 1.721 ± 0.411
3.243ThrSer: 3.243 ± 0.455
2.978ThrThr: 2.978 ± 0.427
3.772ThrVal: 3.772 ± 0.463
0.397ThrTrp: 0.397 ± 0.164
2.118ThrTyr: 2.118 ± 0.315
0.0ThrXaa: 0.0 ± 0.0
Val
4.302ValAla: 4.302 ± 0.548
0.596ValCys: 0.596 ± 0.217
3.904ValAsp: 3.904 ± 0.667
5.294ValGlu: 5.294 ± 0.613
2.449ValPhe: 2.449 ± 0.46
3.971ValGly: 3.971 ± 0.677
0.993ValHis: 0.993 ± 0.333
4.632ValIle: 4.632 ± 0.462
5.559ValLys: 5.559 ± 0.589
3.971ValLeu: 3.971 ± 0.542
2.25ValMet: 2.25 ± 0.373
3.309ValAsn: 3.309 ± 0.497
1.853ValPro: 1.853 ± 0.36
2.25ValGln: 2.25 ± 0.359
2.581ValArg: 2.581 ± 0.471
4.302ValSer: 4.302 ± 0.564
3.904ValThr: 3.904 ± 0.68
4.434ValVal: 4.434 ± 0.526
0.728ValTrp: 0.728 ± 0.274
1.721ValTyr: 1.721 ± 0.346
0.0ValXaa: 0.0 ± 0.0
Trp
0.794TrpAla: 0.794 ± 0.28
0.066TrpCys: 0.066 ± 0.067
0.993TrpAsp: 0.993 ± 0.284
1.125TrpGlu: 1.125 ± 0.401
0.529TrpPhe: 0.529 ± 0.186
0.397TrpGly: 0.397 ± 0.174
0.529TrpHis: 0.529 ± 0.217
0.86TrpIle: 0.86 ± 0.252
0.86TrpLys: 0.86 ± 0.205
0.728TrpLeu: 0.728 ± 0.223
0.463TrpMet: 0.463 ± 0.171
0.794TrpAsn: 0.794 ± 0.236
0.066TrpPro: 0.066 ± 0.068
0.265TrpGln: 0.265 ± 0.153
0.529TrpArg: 0.529 ± 0.181
0.529TrpSer: 0.529 ± 0.257
0.794TrpThr: 0.794 ± 0.237
0.596TrpVal: 0.596 ± 0.18
0.132TrpTrp: 0.132 ± 0.115
0.331TrpTyr: 0.331 ± 0.181
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.985TyrAla: 1.985 ± 0.429
0.331TyrCys: 0.331 ± 0.124
1.919TyrAsp: 1.919 ± 0.42
3.838TyrGlu: 3.838 ± 0.713
1.985TyrPhe: 1.985 ± 0.504
2.118TyrGly: 2.118 ± 0.36
0.463TyrHis: 0.463 ± 0.201
2.713TyrIle: 2.713 ± 0.339
3.375TyrLys: 3.375 ± 0.495
3.044TyrLeu: 3.044 ± 0.443
1.324TyrMet: 1.324 ± 0.307
2.316TyrAsn: 2.316 ± 0.458
0.596TyrPro: 0.596 ± 0.221
1.654TyrGln: 1.654 ± 0.333
2.449TyrArg: 2.449 ± 0.382
1.654TyrSer: 1.654 ± 0.35
2.118TyrThr: 2.118 ± 0.385
2.118TyrVal: 2.118 ± 0.375
0.331TyrTrp: 0.331 ± 0.151
2.25TyrTyr: 2.25 ± 0.457
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.066XaaAsp: 0.066 ± 0.067
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.066XaaIle: 0.066 ± 0.067
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (15112 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski