Mycobacterium phage BuzzLyseyear

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088FQU3|A0A088FQU3_9CAUD Immunity repressor OS=Mycobacterium phage BuzzLyseyear OX=1536598 GN=PBI_BUZZLYSEYEAR_50 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 6.3SIDD5 pKa = 4.49TDD7 pKa = 3.71EE8 pKa = 4.56QLNGYY13 pKa = 8.47PPNTVILDD21 pKa = 3.65ADD23 pKa = 3.36EE24 pKa = 4.41WAWQKK29 pKa = 11.11DD30 pKa = 4.24VNQGSHH36 pKa = 5.75LWIPAAFYY44 pKa = 11.28SEE46 pKa = 5.12LLVDD50 pKa = 4.39DD51 pKa = 5.19GGRR54 pKa = 11.84PSLPARR60 pKa = 11.84LLYY63 pKa = 10.51VPHH66 pKa = 7.11ANSANEE72 pKa = 4.01SS73 pKa = 3.51

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088FU94|A0A088FU94_9CAUD Uncharacterized protein OS=Mycobacterium phage BuzzLyseyear OX=1536598 GN=PBI_BUZZLYSEYEAR_87 PE=4 SV=1
MM1 pKa = 8.0PDD3 pKa = 3.22RR4 pKa = 11.84YY5 pKa = 10.94GEE7 pKa = 4.23TTPEE11 pKa = 3.57PRR13 pKa = 11.84VFVRR17 pKa = 11.84PKK19 pKa = 10.42VNALTVRR26 pKa = 11.84CSWCKK31 pKa = 9.72AGVGARR37 pKa = 11.84CVVAGTNLVLQRR49 pKa = 11.84SSFHH53 pKa = 5.62EE54 pKa = 3.84ARR56 pKa = 11.84VRR58 pKa = 11.84AAEE61 pKa = 3.98LAATGALTRR70 pKa = 11.84GRR72 pKa = 11.84MSS74 pKa = 2.99

Molecular weight: 8.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

19061

30

1174

173.3

18.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.32 ± 0.455

1.249 ± 0.182

6.663 ± 0.224

5.902 ± 0.323

2.891 ± 0.195

8.803 ± 0.536

2.324 ± 0.203

4.323 ± 0.185

3.452 ± 0.169

7.392 ± 0.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.115

3.326 ± 0.16

5.965 ± 0.198

3.578 ± 0.22

6.915 ± 0.337

5.897 ± 0.249

6.511 ± 0.249

7.418 ± 0.287

2.193 ± 0.134

2.639 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski