Purpureocillium lilacinum (Paecilomyces lilacinus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Ophiocordycipitaceae; Purpureocillium

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11750 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A179H7P0|A0A179H7P0_PURLI GTPase activating protein OS=Purpureocillium lilacinum OX=33203 GN=VFPBJ_00237 PE=4 SV=1
MM1 pKa = 7.52PRR3 pKa = 11.84VTSDD7 pKa = 3.13SFSGEE12 pKa = 3.99SAEE15 pKa = 4.22VQRR18 pKa = 11.84DD19 pKa = 3.24QALATKK25 pKa = 10.53LDD27 pKa = 3.74DD28 pKa = 4.69EE29 pKa = 4.72YY30 pKa = 11.65SEE32 pKa = 5.2KK33 pKa = 11.03YY34 pKa = 10.58LDD36 pKa = 4.17GLTILLDD43 pKa = 3.6YY44 pKa = 11.16LNAGNTYY51 pKa = 11.19DD52 pKa = 4.75DD53 pKa = 4.35MLRR56 pKa = 11.84VIPDD60 pKa = 3.17AQEE63 pKa = 3.25WCRR66 pKa = 11.84EE67 pKa = 4.1YY68 pKa = 11.06FSGRR72 pKa = 11.84MVAPNRR78 pKa = 11.84LNEE81 pKa = 4.36EE82 pKa = 4.0VLFWHH87 pKa = 7.08PNLLDD92 pKa = 3.79LSLPSWRR99 pKa = 11.84RR100 pKa = 11.84LDD102 pKa = 3.75GPNNFDD108 pKa = 3.25EE109 pKa = 4.63TQLFGDD115 pKa = 4.41VDD117 pKa = 3.63PMEE120 pKa = 4.51GFEE123 pKa = 5.88YY124 pKa = 10.37PLTWEE129 pKa = 4.7SEE131 pKa = 4.2DD132 pKa = 3.37EE133 pKa = 4.34TLVVEE138 pKa = 5.14MDD140 pKa = 2.96QHH142 pKa = 6.23MNDD145 pKa = 3.42DD146 pKa = 3.74VYY148 pKa = 11.57AFEE151 pKa = 5.75LGQHH155 pKa = 6.0VDD157 pKa = 2.71NDD159 pKa = 3.97YY160 pKa = 11.61NFAEE164 pKa = 4.45AEE166 pKa = 4.11MGHH169 pKa = 5.26VAEE172 pKa = 5.56AVFSDD177 pKa = 4.01GGYY180 pKa = 10.15QINGDD185 pKa = 4.25GISDD189 pKa = 4.06NLDD192 pKa = 3.38LDD194 pKa = 4.13AVLPIYY200 pKa = 10.27GAISEE205 pKa = 4.59WVPFSDD211 pKa = 5.31DD212 pKa = 3.42EE213 pKa = 4.38VHH215 pKa = 7.21AFLDD219 pKa = 3.79RR220 pKa = 11.84HH221 pKa = 4.86SHH223 pKa = 4.67RR224 pKa = 11.84TYY226 pKa = 11.32EE227 pKa = 3.88NLLAGGHH234 pKa = 6.62DD235 pKa = 3.78VLFDD239 pKa = 4.62DD240 pKa = 4.92YY241 pKa = 11.57LFLRR245 pKa = 11.84GAPLMASDD253 pKa = 4.26LTRR256 pKa = 11.84AVAYY260 pKa = 8.35NLPLILSCLASIPLHH275 pKa = 5.66EE276 pKa = 4.62VVDD279 pKa = 4.58LDD281 pKa = 3.93VLGMEE286 pKa = 4.49TDD288 pKa = 4.19DD289 pKa = 5.36NEE291 pKa = 4.58STT293 pKa = 3.81

Molecular weight: 33.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A0A179GC70|LCSQ_PURLI Nucleotidyltransferase lcsQ OS=Purpureocillium lilacinum OX=33203 GN=lcsQ PE=2 SV=1
MM1 pKa = 7.38FSGRR5 pKa = 11.84RR6 pKa = 11.84TRR8 pKa = 11.84RR9 pKa = 11.84ANAHH13 pKa = 5.74HH14 pKa = 6.57HH15 pKa = 5.87HH16 pKa = 6.6HH17 pKa = 6.58HH18 pKa = 6.41HH19 pKa = 5.98TTTTTTRR26 pKa = 11.84APRR29 pKa = 11.84RR30 pKa = 11.84SIFSRR35 pKa = 11.84RR36 pKa = 11.84QRR38 pKa = 11.84VAVHH42 pKa = 4.84HH43 pKa = 5.95QKK45 pKa = 10.56RR46 pKa = 11.84KK47 pKa = 8.39PTMKK51 pKa = 10.55DD52 pKa = 3.11KK53 pKa = 11.05ISGALTKK60 pKa = 10.7LRR62 pKa = 11.84GSLTRR67 pKa = 11.84RR68 pKa = 11.84PGLKK72 pKa = 10.1AAGTRR77 pKa = 11.84RR78 pKa = 11.84MHH80 pKa = 5.68GTDD83 pKa = 2.72GRR85 pKa = 11.84GSHH88 pKa = 6.15RR89 pKa = 11.84AGRR92 pKa = 11.84RR93 pKa = 11.84FFF95 pKa = 4.56

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11750

0

11750

5417339

45

11859

461.1

50.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.846 ± 0.023

1.34 ± 0.01

5.902 ± 0.017

5.789 ± 0.023

3.529 ± 0.013

7.433 ± 0.02

2.441 ± 0.012

4.224 ± 0.016

4.445 ± 0.02

8.74 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.008

3.218 ± 0.014

6.2 ± 0.027

3.928 ± 0.019

6.687 ± 0.023

7.832 ± 0.025

5.744 ± 0.017

6.394 ± 0.016

1.525 ± 0.009

2.552 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski