Miniopterus associated gemycircularvirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus minio1

Average proteome isoelectric point is 5.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3MDS0|A0A0D3MDS0_9VIRU Replication-associated protein OS=Miniopterus associated gemycircularvirus 1 OX=1985387 PE=4 SV=1
MM1 pKa = 7.51GCLGGASLGVGMQAHH16 pKa = 7.1LSAPLGAGAAAHH28 pKa = 6.69GGPTSTLVDD37 pKa = 4.42FLTEE41 pKa = 3.91RR42 pKa = 11.84PMSLVLWGPTRR53 pKa = 11.84TGKK56 pKa = 9.03TSWARR61 pKa = 11.84SLGPHH66 pKa = 7.54AYY68 pKa = 9.16TMGMVSGNKK77 pKa = 9.57LLADD81 pKa = 3.91MEE83 pKa = 4.45DD84 pKa = 3.16AEE86 pKa = 4.63YY87 pKa = 11.04AVFDD91 pKa = 4.56DD92 pKa = 4.22MRR94 pKa = 11.84GGMKK98 pKa = 9.81FFPAWKK104 pKa = 9.19EE105 pKa = 3.46WFGAQKK111 pKa = 10.67VVTIKK116 pKa = 10.66CLYY119 pKa = 10.39KK120 pKa = 10.63EE121 pKa = 4.1PQQVNWGKK129 pKa = 9.84PIIWISNKK137 pKa = 9.96DD138 pKa = 3.44PRR140 pKa = 11.84EE141 pKa = 4.11DD142 pKa = 3.05MDD144 pKa = 3.86MGDD147 pKa = 4.04TEE149 pKa = 4.37WLEE152 pKa = 4.33GNASIVYY159 pKa = 10.25VGDD162 pKa = 3.74SLISRR167 pKa = 11.84ASTEE171 pKa = 3.76

Molecular weight: 18.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3MDS0|A0A0D3MDS0_9VIRU Replication-associated protein OS=Miniopterus associated gemycircularvirus 1 OX=1985387 PE=4 SV=1
MM1 pKa = 7.71PFKK4 pKa = 10.55FQSRR8 pKa = 11.84YY9 pKa = 9.78VLLTYY14 pKa = 8.03SQCGEE19 pKa = 4.37LDD21 pKa = 3.4PFDD24 pKa = 4.25VVNHH28 pKa = 6.39LSSLGAEE35 pKa = 4.35CIVGRR40 pKa = 11.84EE41 pKa = 3.92PHH43 pKa = 6.86ADD45 pKa = 3.28GGTHH49 pKa = 6.2LHH51 pKa = 6.48AFVDD55 pKa = 4.57FGRR58 pKa = 11.84RR59 pKa = 11.84YY60 pKa = 7.34STRR63 pKa = 11.84RR64 pKa = 11.84SDD66 pKa = 3.36AFDD69 pKa = 3.04VGGYY73 pKa = 9.35HH74 pKa = 7.24PNIAPSRR81 pKa = 11.84GRR83 pKa = 11.84AEE85 pKa = 4.5GGWDD89 pKa = 3.43YY90 pKa = 10.96ATKK93 pKa = 10.59EE94 pKa = 3.88GDD96 pKa = 3.21IVAGGLEE103 pKa = 4.11RR104 pKa = 11.84PSRR107 pKa = 11.84SRR109 pKa = 11.84VSRR112 pKa = 11.84NDD114 pKa = 4.08HH115 pKa = 5.97IWDD118 pKa = 3.7EE119 pKa = 4.77VINQPDD125 pKa = 3.43EE126 pKa = 4.19SAFWEE131 pKa = 4.8CVQRR135 pKa = 11.84LDD137 pKa = 3.9PKK139 pKa = 10.84AAATQFTQLRR149 pKa = 11.84AYY151 pKa = 10.2ASWAYY156 pKa = 9.98AVQPEE161 pKa = 4.39QYY163 pKa = 10.03EE164 pKa = 4.4PNPEE168 pKa = 4.03HH169 pKa = 7.15AFDD172 pKa = 3.46TGMVPEE178 pKa = 4.42LDD180 pKa = 2.75RR181 pKa = 11.84WRR183 pKa = 11.84RR184 pKa = 11.84EE185 pKa = 3.71SLGDD189 pKa = 3.55DD190 pKa = 3.44RR191 pKa = 11.84QGTFAFSRR199 pKa = 11.84SGRR202 pKa = 11.84KK203 pKa = 8.94RR204 pKa = 11.84PALYY208 pKa = 10.4RR209 pKa = 11.84NRR211 pKa = 3.78

Molecular weight: 24.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

382

171

211

191.0

21.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.162 ± 0.113

1.309 ± 0.081

6.806 ± 0.558

6.021 ± 0.441

4.188 ± 0.736

10.209 ± 1.206

2.618 ± 0.503

2.88 ± 0.366

3.403 ± 1.083

6.806 ± 0.804

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.141 ± 1.576

2.618 ± 0.162

5.759 ± 0.289

3.403 ± 0.62

7.592 ± 2.377

7.068 ± 0.029

4.712 ± 0.661

5.759 ± 0.052

3.141 ± 0.554

3.403 ± 0.62
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski